19
Figure 15. Objective Heater Calibration Page
The temperature of the Objective Heater is factory calibrated at room temperature of 23°C, with a 3
degrees offset relative to the sample set-point temperature of 37°C. This offset value is appropriate for a
set point of 37°C. If your set point is different than 37°C the system will automatically apply the correct
offset. See Figure 15 above. This means that if the temperature is set at 37°C the Objective Heater is kept
at 40°C in order to maintain the sample at the desired temperature while imaging with an oil immersion
ob
jective. To accept this offset you can press “USE FACTORY” and return to the Homepage. Alternatively
the following options are available:
8.
MANUAL: Selecting “MANUAL” allows searching for the best offset value manually, step by step. See
Figure 16. Objective Heater Manual Offset
6.2.1.3
Thermal Cycles
This function allows the incubator to switch between two different temperatures (both temperatures must
be at least 3°C above Room Temperature).
