OM0303
Rev 5 July/2021
Page 35 of 108
6.3.5
Perform Work
The work can now be performed. The technician performing the work is encouraged to wear a long-sleeved gown
with knit cuffs and rubber gloves. This will minimize the shedding of skin flora into the work area and concurrently
protect the hands and arms from viable agent contamination. At a minimum, the hands and arms should be
washed well with germicidal soap before and after work in the cabinet. For the preparation of antineoplastic
drugs, the following procedures summarize those contained in OSHA
’s guidelines to controlling occupational
exposure to hazardous drugs. The above document should be thoroughly studied and reviewed prior to drug
preparation in the cabinet. It may be found at this website:
https://www.osha.gov/SLTC/hazardousdrugs/controlling_occex_hazardousdrugs.html.
a. A sterile plastic-backed absorbent drape should be placed on the work surface during mixing procedures.
The drape should be exchanged whenever significant spillage occurs, or at the end of each production
sequence.
b. Vials should be vented with a filter needle to eliminate internal pressure or vacuum.
c. Before opening ampoules, care should be taken to ensure that no liquid remains in the tip of the
ampoule. A sterile gauze sponge should be wrapped around the neck of the ampoule while opening.
d. Final drug measurement should be performed prior to removing the needle from the stopper of the vial.
e. A non-splash collection vessel should be available in the Biosafety cabinet to discard excess drug
solutions.
6.3.6
Terminal Purging & Wipe Down
Following completion of work, allow the cabinet to run for 2-3-minute period without personnel activity to purge
the cabinet. A surface disinfection of the interior surfaces (see Cleaning Procedures section) should be repeated
after removal of all materials, cultures, apparatus, etc. A careful check of grills and diffuser grids should be made
for spilled or splashed nutrients which may support fungus growth and resulting spore liberation that
contaminates the protected work environment.
6.3.7
Paper Catch/Prefilter
A permanent paper catch is installed behind the rear divider panel of the work zone. This area forms the return air
path to the motor/blower; and if the airflow is blocked, it could seriously affect the performance of the cabinet.
Therefore,
THE PAPER CATCH SHOULD BE CHECKED AND CLEANED NO LESS THAN ON A WEEKLY BASIS;
daily
basis if procedures dictate the use of paper products. Any paper removed must be properly disposed of as
Contaminated Hazardous Waste.
The above procedures also apply to all cabinets configured with a prefilter.
6.3.8
Shut Down
Turn off blowers and lights. Do not use cabinet as a depository for excess lab equipment during periods of non-
operation. If antineoplastic agents are being prepared in the cabinet, it is recommended to let the cabinet run 24
hours per day. This lessens the possibility that contaminants may escape.
Summary of Contents for LabGard NU-581-400E
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