NuAire LabGard NU-540-300E Operation And Maintenance Manual Download Page 1

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OM0280

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LabGard® ES Energy Saver

Class II Laminar Flow

Biosafety Cabinet

Models

NU-540-300E/400E/500E/600E

Bench/Console

Operation and Maintenance Manual

July, 2021

Revision 3

NU-540-300E/400E/500E/600E Series F

Manufactured By:

NuAire, Inc.

2100 Fernbrook Lane

Plymouth, MN 55447

Toll-Free: 1-800-328-3352

In Minnesota: (763)-553-1270

Fax: (763)-553-0459

Summary of Contents for LabGard NU-540-300E

Page 1: ...abinet Models NU 540 300E 400E 500E 600E Bench Console Operation and Maintenance Manual July 2021 Revision 3 NU 540 300E 400E 500E 600E Series F Manufactured By NuAire Inc 2100 Fernbrook Lane Plymouth...

Page 2: ...m particulate contaminants as prescribed in EN 12469 and NSF ANSI 49 Please read this manual carefully to familiarize yourself with proper installation maintenance and operation of the cabinet Other r...

Page 3: ...ings are appended in the back flap of this manual along with any modifications and or additions to procedures as outlined in this manual A copy of the original factory test report is also appended to...

Page 4: ...eak test 7 7 Airflow Smoke Pattern Test 7 8 Site Installation Assessment Test 7 9 Cleanliness Classification Test for Pharmacy Application 7 10 Main Control Board Description and Replacement Section N...

Page 5: ...Airflow Schematic and the application of environmental controls as required in the field of biological research or chemical containment The LFBSC when used with proper technique is an effective primar...

Page 6: ...while specific agents are used o which measures are to be taken in the case of an accident Repairs to the device must be carried out only by trained and authorized expert personnel Keep these operatin...

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Page 8: ...OM0280 Page 8 of 63 Rev 3 July 2021 2 0 Models and Features The model NU 540E LabGard ES Class II Laminar Flow Biosafety Cabinet is manufactured in four sizes 3 ft 9m 4 ft 1 2m 5 ft 1 5m and 6 ft 1 8m...

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Page 13: ...Shipments 4 1 1 Terms are factory unless stated otherwise Therefore it is important to check each shipment before acceptance 4 1 2 If there is visible damage the material can be accepted after the dri...

Page 14: ...aintenance purposes Do not position the cabinet to prevent access to the power cord The power cord plug serves as the disconnect and should remain readily accessible If the outlet is inaccessible such...

Page 15: ...ENTS i e locking type OF DRAIN VALVE INSTALLATION remove the handle from the valve stem to gain clearance for valve body rotation Add Loctite 242 furnished to the threads and rotate valve body until s...

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Page 17: ...uld not be used Do not attempt to clean the HEPA filter media Cabinet interior walls or work surface are easily cleaned with any mild household detergent cleaner using a soft cloth Turn the cabinet on...

Page 18: ...Sidewall One Backwall Left or Right Work Surface Cabinet Size Inches mm Height Fully Assembled Height Minimum for Transport Width Depth with Armrest Removed 60 7 8 1546 59 1499 41 5 8 1057 31 7 16 79...

Page 19: ...compensation constant volume control for both filter loading and line voltage variances Monitor and display airflow system performance via PresurFlow monitor The LabGard ES NU 540E offers the latest d...

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Page 21: ...bove the key indicates full blue for fluorescent light on 6 1 2 4 Ultraviolet UV Light Key The UV light key controls the on off power to the UV light if optionally installed LED above indicates full y...

Page 22: ...e cabinet airflow status During the warm up period the aseptic cleaning process may begin If the sliding window is raised an audible and visual alarm will occur but may be silenced by pressing the ala...

Page 23: ...r expires the function will turn off Select auto timer duration function o Outlets Press and hold key then press hidden outlet outlet keys sequentially LED indicator above outlet will blink fast Adjus...

Page 24: ...blower hidden keys sequentially Red LED indicator above blower will blink fast Select password Press or key to scroll through the code choices below No Password B B H password B H B password default B...

Page 25: ...should be evaluated by appropriate safety personnel 6 2 2 Minimize Penetration of Air Curtain The minimum number of items necessary should be placed into the cabinet to prevent overloading but the wor...

Page 26: ...precaution is merely an extension of good aseptic technique as practiced on open bench tops The standard laboratory practices designed to minimize creation and or release of aerosols to the environmen...

Page 27: ...lls Materials should be arranged so that clean dirty used and virus materials are well separated Passage of contaminated materials over uninoculated cultures or clean glassware should be avoided and t...

Page 28: ...re feet are resting on the floor chair foot support or foot rest Also be sure back is fully supported with proper chair adjustments Forearm elbow support The cabinet is provided with a non metallic fo...

Page 29: ...I LFBSC can be found in the 2006 ASHP Technical Assistance Bulletin ASHP Guidelines on Handling Hazardous Drugs2 6 6 2 Procedure a Make sure that the cabinet remains in operational mode with internal...

Page 30: ...ly decontaminated is known to be biologically clean or known to be chemically inert Surface disinfection is performed as specified in the Cleaning Procedures section Hazardous Gases Personal Protectio...

Page 31: ...E SURE CABINET IS TOTALLY SEALED TO PREVENT ANY LABORATORY EXPOSURE TO DECONTAMINATION GAS 9 Perform decontamination procedure Please consult with NuAire Inc about any unique contamination problems No...

Page 32: ...lock washers The screws have O rings and should be replaced if damaged or badly deformed The interior of the cabinet is now fully exposed for replacement of the filters and motor blower The lowest wi...

Page 33: ...x 32 813mm x 3 76mm 20 508mm x 14 366mm x 6 152mm Filter Manufacturer Camfil Farr Camfil Farr NU 540 400E NuAire Part Number A 980979 01 A 980957 18 Filter Size 21 533mm x 44 1118mm x 3 76mm 20 508mm...

Page 34: ...top of the hood Tighten the spring loaded bolts 4 places depressing the gasket material by 1 8 inch 3mm Step 5 Motor Blower Assembly Removal a It is recommended that the motor blower to be removed as...

Page 35: ...perly activate the micro switches thus causing potential operational malfunctions to occur If the window counter balance experiences a fault window function must be verified the sliding window retenti...

Page 36: ...model size Class II 3 foot Class II 4 foot Class II 5 foot Class II 6 foot Laminar 3 4 foot Laminar 5 6 foot o Press hidden key to accept model size and exit o Press key at any time to abort and exit...

Page 37: ...adjusts or balances the exhaust airflow as well as makes up for filter resistance tolerances Since it has been NuAire s experience that the filters may not load evenly choke adjustments may be necess...

Page 38: ...justment 7 5 3 2 Inflow Calibration Step 1 Measure the inflow velocity using the recommended procedure found in Table 7 0 If necessary adjust the exhaust filter choke located under the front decorativ...

Page 39: ...must be non blinking to save or exit Note At any time during the process Press hidden key to accept low alarm setpoint value Press and hold outlet key for three seconds to remove any previous offsets...

Page 40: ...not go through normal start up procedure 7 5 3 5 Nite Care Calibration See Section 8 2 2 to Activate the Nite Care Function The Nite Care mode is defaulted to operate the blower at approximately 600 r...

Page 41: ...18 750 476 18 0 457 17 750 451 17 5 445 Number of Readings Average Velocity ft min m s d Acceptance Criteria 1 Average downflow velocity 55 to 65 fpm 28 to 33 m s 2 Individual readings must be within...

Page 42: ...3 386 37 584 41 782 45 980 50 178 54 375 102 208 315 421 528 635 741 848 955 1061 1168 1274 1381 600E 4 8 158 12 316 16 474 20 632 24 790 28 948 33 106 37 264 41 422 45 580 49 738 53 896 58 054 62 212...

Page 43: ...fficult to check since protective grills charcoal filters or exhaust transitions could cover the filter Access panels are usually provided and should be removed If an air gap exhaust transition is pro...

Page 44: ...shall show smooth downward flow with no dead spots or reflux 2 No smoke shall escape from inside the cabinet 3 No smoke refluxes out of the cabinet once drawn in nor does smoke billow over the worksur...

Page 45: ...ts certified operating voltage as measured by the voltmeter 7 8 3 Exhaust System Performance Canopy Exhaust Transitions Step 1 Introduce a visible medium source into the canopy air intake s while slow...

Page 46: ...test using non hazardous materials A minimum of three 3 1 minute particle counts shall be sampled and recorded while the user simulates aseptic compounding manipulations At Rest Particle Count Test Ta...

Page 47: ...MPS 2 AMPS 1 AMP 2 NU 540 400E 6 25 AMPS 3 AMPS 2 AMPS 1 AMP 2 NU 540 500E 8 AMPS 3 AMPS 2 AMPS 1 AMP 2 NU 540 600E 8 AMPS 3 AMPS 2 AMPS 1 AMP 2 7 10 3 Main Control Board Replacement Note All setup an...

Page 48: ...he initial power up the RED alarm LED will blink to indicate power up status 5 Press key to clear the power on reset message 6 Enter into service parameters and input configuration for calibration and...

Page 49: ...utlet fuse located on main control board Check voltage coming out of main control board Cabinet ultraviolet light won t turn on Yellow LED above UV light key indicates the UV lamp should be on Check s...

Page 50: ...running for the accessary outlet to turn on Sync Outlet Power with Active Blower Normally the outlet power is turned on via the outlet key If the outlet power sync is active the outlet power will turn...

Page 51: ...running Upon closure the blower will continue to run at a calibrated lower speed level to keep the workzone interior sterile The PresurFlow will indicate s blinking green LEDs along with green LED ab...

Page 52: ...re activated whenever the blower key is pressed and the blower key LED indicator is on or blinking Contact ratings are 250 VAC maximum at 2 Amps 9 2 Alarm Relay The alarm relay contacts are normally o...

Page 53: ...June 1976 Since ultraviolet rays will not penetrate ordinary glass it is recommended that the sliding window be closed while the ultraviolet light is on within the cabinet or that personnel leave the...

Page 54: ...form the following Press and hold key the press UV light UV light UV light key sequentially and the reset will occur removing the above alarm condition It is also possible to approximate the UV light...

Page 55: ...ep hermolyticus alpha type 5 500 Staphylococcus aureus 6 600 Staphylococcus albus 5 720 Micrococcus sphaeroides 15 400 Sarcina lutea 26 400 Pseudomonas fluorescens 7 040 Escherichia coli 7 040 Proteus...

Page 56: ...lic mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply 11 5 Pollution Degre...

Page 57: ...t Grill Stainless Steel Worksurface Stainless Steel Window Faring Stainless Steel Window Glides HDPE Window Safety Glass Window Frame Stainless Steel Front Service Panel Painted Steel Front Decorative...

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