TEC-B-01 User Manual
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version 1.1
page 23
7. Sample Experiment
In the following the basics of a simple experiment are described. It is assumed that all connections are
built as described in chapter 4.3. Before starting remove the cell model.
Again
: It is of major importance that the TEC-03X systems are used only in warmed-up condition i.e.
20 to 30 minutes after turning power on.
Figure 9: model circuit for voltage clamp recording from a cell, e.g. an oocyte using low-resistance
electrodes
C
m
: membrane capacity, R
m
: membrane resistance, R
CEL
: current electrode resistance, R
PEL
: potential
electrode resistance, R
REF
: reference electrode resistance, R
s
: series resistance
Adjust BIAS CURRENT to 0 if necessary (see chapter 0).
Turn off the amplifier!
Reconnect the COMMAND INPUT.
Connect the Ag-AgCl pellet or the agar-bridge for grounding the bath with GND at the potential
headstage.
Connect the reference electrode to REF. at the potential headstage (High Voltage TEC systems
only).
Insert potential- and current electrode into the electrode holders. Check if the silver wires of both
electrodes are well chlorided and in contact with the electrode solution, and connect them to the
respective headstages.
Immerse both electrodes into the bath and make the basic settings (see chapter 4.2).
Turn on the amplifier.
