TEC-B-01 User Manual
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version 1.1
page 10
3.2.
Operation with Bridge Amplifier
Figure 2: front panel of BA-03X
Together with a modified bridge amplifier the TEC-B-01 can be used for two electrode voltage clamp
(TEVC) or two electrode current clamp (TECC) experiments. The scaling is adapted for current ranges
normally used in experiments with oocytes.
Figure 3: Rear panel connector of the BA-03X for connection with TEC-B-01 (left). A DUAL LED on
the front panel of the BA-03X indicates two electrode operation (right).
For two electrode operation the TEC-B-01 and bridge amplifier are connected with a cable at the rear
panel. The operation mode switch (#
1
) is set to CC (for current clamp) or VC (for voltage clamp). The
current electrode is connected to the TEC-B-01 headstage and the potential electrode to the headstage
of the bridge amplifier.
In two electrode operation current injection is done with the current electrode (connected to the TEC-
B-01) and potential measurement with the potential electrode (connected to the bridge amplifier. This
applies to both CC and VC mode.
Two electrode operation is indicated by the DUAL LED at the TEC-01-B as well as by the DUAL
LED at the bridge amplifier. Current display and current output at the bridge amplifier are disabled.
Voltage clamp:
In voltage clamp mode, the command signal is fed into the COMMAND IN BNC connector (
#15
) at
the TEC-B-01 front panel.
Current clamp:
In current clamp mode, the command signal is fed into one of the STIMULUS INPU BNC connectors
(
#23
or
#24
) at the BA-03X front panel.
3.3.
BUZZ or Penetration mode
In two electrode configuration, the BUZZ module of the BA-03X works for both the
potential electrode from BA-03X (P
EL
) and the current electrode from TEC-B-01 (C
EL
).
The BUZZ select switch at the BA-03X front panel allows selection which electrode the
BUZZ shall be applied to.
