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Chapter 4

Functions and Operations of the Devices

48

11

Epi-Fluorescence Attachment

This microscope system can be used with the P-EFL Epi Fluorescence Attachment to perform epi-fluorescence microscopy

11.1

P-EFL Epi Fluorescence Attachment

This epi-fluorescence attachment is used with filter
cubes attached to the turret inside the attachment.
Bring the desired filter cube into the optical path
when performing epi-fluorescence microscopy.
Install this attachment between the zooming body
and the tube.

An HG precentered fiber illuminator is used as the
light source. The light from the light source is
introduced inside the attachment and led through
the filter cubes attached to the turret for
epi-fluorescence illumination. (For details on how to
use the light source, refer to the instruction manual
supplied with the light source.)

Up to four cubes can be attached to the turret of the
epi-fluorescence attachment. Turn the turret using
the filter cube switching knob. Align a turret address
number (1 to 4) displayed on the knob with the
indicator to bring the filter cube of the selected
address into the optical path.

INTENSILIGHT

C-HGFI

LAMP

ND

1

2

4

32

16

8

SHUTTER

POWER

RUN TIM

E hrs.

P-EFL Epi Fluorescence Attachment

CAUTION



Do not use the epi-fluorescence attachment while the filter cube replacement cover is open. Harmful light including ultraviolet

light may leak and affect fluorescence observation.



Immediately after diascopic observation using an epi-fluorescence attachment, the front face of the empty cube may become

hot, resulting in burns. Do not touch the front face of the empty cube. Replace the empty cube several minutes after finishing
the diascopic observation.

Be sure to attach four cubes to the turret.

Attach an empty cube at positions where no filter cube is required.

C-HGFI HG Precentered

Fiber Illuminator

Filter cube
replacement hole
cover

Turret address

indication

Filter cube indication
label
(label attachment
position)

Index mark

Filter cube
switching knob

Shielding

plate

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Summary of Contents for SMZ1270

Page 1: ...Stereo Microscope SMZ800N SMZ1270 SMZ1270i Instruction Manual M609 E 18 5 NF 7 1 2 M609EN07 ...

Page 2: ......

Page 3: ... any points that are unclear or incorrect please contact your nearest Nikon representative Some of the equipment described in this manual may not be included in the set you have purchased If you intend to use any other equipment with this product read the manual for that equipment too If this equipment is used in a manner not specified by the manufacturer the protection provided by the equipment m...

Page 4: ...7 Focusing on the Sample 14 8 Changing the Magnification 16 8 1 Image Zooming 16 8 2 Switching Objectives Using a Nosepiece 17 9 Shifting a Sample 18 9 1 Sliding the Stage Plate 18 9 2 Shifting the Stage in the X and Y Directions P SXY64 XY Stage 18 9 3 Using Other Stages 18 10 Using Episcopic Illumination 19 11 Using Epi Fluorescence Illumination 20 11 1 Switching Filter Cubes 20 11 2 Selecting a...

Page 5: ...42 8 4 C TRS Tilting Stage 42 9 Tubes 43 9 1 P B Binocular Tube 43 9 2 Trinocular Tubes 44 10 Episcopic Illuminators 45 10 1 P CI Coaxial Epi Illuminator 45 10 2 C FDF Flexible Double Arm Fiber Illumination Unit 46 10 3 C FID2 Double Arm Fiber Illuminator 46 10 4 C FIR Ring Fiber Illumination Unit 47 10 5 Other Episcopic Illuminators 47 11 Epi Fluorescence Attachment 48 11 1 P EFL Epi Fluorescence...

Page 6: ...ooting 83 1 Image Viewing 84 2 Operation 84 Maintenance and Storage 85 1 Cleaning 86 1 1 Cleaning Lenses 86 1 2 Cleaning Parts Other than the Lens 86 1 3 Disinfecting the Product 87 2 Storage 87 3 Periodic Inspection Charged 87 Specifications 89 1 Microscopy Principles 90 2 Performance Properties 90 3 Physical Properties 96 ...

Page 7: ...l may lead to injury or property damage Label Attached to the Product The label attached to the products shown below indicates that attention should be paid to use of these products Before using a product with the label attached be sure to carefully read the manual Photobiological safety label IEC62471 RISK GROUP2 Possibly hazardous optical radiation emitted from this product Do not stare at opera...

Page 8: ...ile the filter cube replacement cover is open Harmful light including ultraviolet light may leak and affect fluorescence observation Label Position P EFL Epi Fluorescence Attachment Drop caution label Caution Use of anti drop collar When using a C FMCN Focus Mount attached in the middle of the support pillar be sure to use the anti drop collar Otherwise the zooming body may slide down when the foc...

Page 9: ... Stand P DSL32 LED Diascopic Illumination Stand or P DSF32 Fiber Diascopic Illumination Stand Biohazard label Caution Biohazard This symbol calls your attention to the following The product may become biohazardous if a sample is spilled onto the product To avoid exposure to biohazard do not touch contaminated parts with your bare hands Decontaminate the contaminated parts according to the standard...

Page 10: ... power supplied to the device matches the power used by the device If the input rating does not match do not use the power supply and contact your local representative Using an incorrect power supply may damage the device 4 Notes on the power cord Be sure to use the specified power cord Use of other power cords may result in malfunction or fire This product is classified as having Class I protecti...

Page 11: ...essure gas The lamps contain sealed gas under very high pressure And the pressure increases when the lamp is on Crack or dirt of the lamp tube strong external force or impact or use beyond the tube s life may cause the encapsulated gas to leak or the lamp to blow up If this happens an accident such as gas inhalation or injury with glass may take place Heat When the lamp is lit the lamp and surroun...

Page 12: ... stand If the focus mount is attached in the incorrect orientation the stand may fall over This may result in damage to the device or unexpected injury 7 Specification of a light source Be sure to use the specified light source for an illuminator Using a light source not specified can cause malfunction and induce an accident For the specified light source see Chapter 8 2 Performance Properties or ...

Page 13: ...ent used and the height of your chair 14 Cautions on use transportation and storage This product must be operated transported and stored in accordance with the following conditions If the product is installed in a location with high temperature and high humidity mold and or condensation may form on the lenses This may result in performance deterioration or malfunction Operating conditions temperat...

Page 14: ...her bright locations Light from room lights directly above this product may enter the objective as extraneous light If possible switch off the room lights directly above this product when observing samples Select a location with minimal dust To avoid splashes do not use this product near water Make sure the ambient temperature is 0 to 40 C and humidity is 60 RH max at 40 C with no condensation Whe...

Page 15: ...devices and components of the following basic types of system configuration as examples 1 System of an SMZ800N Zooming Body with a C PSN Plain Stand 2 System of an SMZ1270 Zooming Body with a P PS32 Plain Stand For details on the names of the devices in the system combined with an episcopic illuminator or diascopic illumination stand see Chapter 3 Specific Operations and Chapter 4 Functions and Op...

Page 16: ...pport pillar SMZ800N Zooming Body Focus knob C PSN Plain Stand base Sample holder attachment hole Stage plate Zoom knob Illuminator attachment hole Diopter adjustment ring Illuminator attachment screw hole Screw hole for attaching optional devices Focus knob Zoom knob Tool holder rear Illuminator attachment screw hole Screw hole for attaching optional devices ...

Page 17: ...ctive P PS32 Plain Stand support pillar SMZ1270 Zooming Body Fine focus knob Screw holes for attaching optional devices P PS32 Plain Stand base Stage plate Stage plate fixing screw P B Binocular Tube C FMCN Focus Mount Illuminator attachment hole Zoom knob Coarse focus knob Fine focus knob Clamp tool Coarse focus knob torque adjustment ring Illuminator attachment hole ...

Page 18: ...Chapter 1 Components 4 ...

Page 19: ... For details on the procedure for using the system combined with other devices see Chapter 3 Specific Operations and Chapter 4 Functions and Operations of the Devices It is assumed that all required devices are attached to the microscope If assembly of the microscopy system has not yet been completed complete assembly as described in Chapter 5 3 Assembly Procedures ...

Page 20: ... Adjusting the Diopter This adjustment is required for each observer Adjusting the diopter 3 Adjust the interpupillary Adjust the interpupillary to match the distance between your right and left eyes Look into both eyepieces and move the binocular part of the tube so that the right and left fields of view merge into a single circle This adjustment is required for each observer Adjusting the interp...

Page 21: ...ase The magnification continuously changes from 1x to 8x Zoom magnification values are indicated on the zoom knobs Zooming in out Click stop during image zooming It is also possible to use click stops at predetermined magnification positions during image zooming See Chapter 4 1 1 SMZ800N Zooming Body Notes on microscopy procedures when using other system configurations With a system configuration ...

Page 22: ...Chapter 2 Microscopy Procedures 8 ...

Page 23: ...9 Chapter 3 Specific Operations This chapter describes how to use the microscope system to observe samples For details about using the individual devices see Chapter 4 ...

Page 24: ...D brightness control selection switch is turned on unless the LED power ON OFF switch on the stand is turned on P2 CTLB Control Box P DSF32 Fiber Diascopic Illumination Stand Refer to the instruction manual supplied with the C FLED2 LED Light source for fiber illuminator C LEDS Hybrid LED Stand Refer to the instruction manual supplied with the C LEDS Hybrid LED Stand C DS Dia Illumination Stand Re...

Page 25: ...ng the C FLED2 LED Light source for fiber illuminator Refer to the instruction manual supplied with the C FLED2 for details P CI Coaxial Epi Illuminator C FDF Flexible Double Arm Fiber Illumination Unit C FID2 Double Arm Fiber Illuminator C FIR Ring Fiber Illumination Unit P DSF32 Fiber Diascopic Illumination Stand Adjust the brightness using the C HGFI C HGFIE HG Precentered Fiber Illuminator Ref...

Page 26: ...of the right eye is diverted to the vertical tube Images of the right eye s optical path are captured 4 Adjusting the Angle of Depression Changing the Eye Level When a trinocular tilting tube P TERG100 or P TERG50 is used the angle of the binocular part can be changed and adjusted to facilitate microscopy The angle can be steplessly changed by moving the binocular part as indicated by the arrow in...

Page 27: ...SMZ1270 SMZ1270i Zooming Body 0 63x to 8x 4 Repeat steps 2 and 3 to check the focus so that changing the magnification does not affect the focus and clear images can be captured 6 Adjusting the Interpupillary Distance Adjust the interpupillary distance to match the distance between your right and left eyes This adjustment is required for each observer Look into both eyepieces and move the binocula...

Page 28: ... figure shows the turning direction of the focus knobs and the corresponding vertical movement of the zooming body Turning the knob backward Upward movement Turning the knob forward Downward movement Focusing on the sample Notes on turning the focus knobs When using the focus knobs on the C FMCN Focus Mount do not turn or twist the right and left focus knobs in opposite directions at the same time...

Page 29: ...g the fine control knob will cause the coarse control knob to also turn and change the torque of the fine control knob A hex driver is fitted on top of each support pillar of the P PS32 Plain Stand the P DSL32 LED Diascopic Illumination Stand or the P DSF32 Fiber Diascopic Illumination Stand Focusing stroke C FMAN Focus Mount Focusing stroke 40 mm Stroke per focus knob rotation 18 7 mm C FMBN Focu...

Page 30: ... direction and the corresponding changes in magnification Turning the knob backward Magnification increase Turning the knob forward Magnification decrease Magnification and zoom ratio SMZ800N 1x to 8x 8 1 SMZ1270 SMZ1270i 0 63x to 8x 12 7 1 Zooming in out Total magnification The total magnification of the microscope is determined by the formula below Total magnification zoom magnification x object...

Page 31: ... and vertical view A nosepiece has two click positions for each objective The view state can be changed between stereoscopic view and vertical view according to the click position Microscopy can be performed in the vertical position when needed such as when taking images of the sample The click position that an objective is in can be checked by viewing the position of the objective from the front ...

Page 32: ... using the fixing screw when this mechanism does not need to be used 9 2 Shifting the Stage in the X and Y Directions P SXY64 XY Stage Turn the XY shift knob of the P SXY64 XY Stage mounted in place instead of the stage plate to shift the sample on the glass plate and change the part to be observed XY stroke of the stage with the X Y shift knob and stroke per knob rotation X 150 mm 37 5 mm per rot...

Page 33: ...dition if a P CI Coaxial Epi Illuminator is attached illumination can be emitted along the optical axis and this gives good results when observing samples such as metallic mirrors resins with smooth surfaces and semiconductor wafers See Chapter 4 10 Episcopic Illuminators and select the appropriate device based on the objective of the microscopy to be performed P CI Coaxial Epi Illuminator C FDF F...

Page 34: ...perations related to light sources such as brightness adjustment refer to the instruction manuals of the light sources INTENSILIGHT C HGFI LAMP ND 1 2 4 32 16 8 SHUT TER POWER RUN TIME hrs Epi fluorescence illumination with the epi fluorescence attachment WARNING The light source used with the epi fluorescence attachment mercury lamp requires special care during handling because of its characteris...

Page 35: ...to use non fluorescent slide glass or cover glass to obtain a high contrast image Locating a target in the sample For epi fluorescence microscopy Nikon recommends locating a target using bright field diascopic illumination OCC illumination and then switching to epi fluorescence rather than emitting strong excitation light to the sample from the beginning When doing so follow the operational tips b...

Page 36: ...ic illumination stand as follows 1 Set the zoom magnification to the smallest 2 Loosen the fixing knob of the C FMCN Focus Mount 3 Move the C FMCN Focus Mount to align the observation axis with the optical axis of the diascopic illumination stand Be careful not to make the periphery dark 4 Tighten the fixing knob of the C FMCN Focus Mount Diascopic illumination with the P DSL32 LED Diascopic Illum...

Page 37: ...e image on the monitor to move horizontally loosen the attachment guide fixing screw and adjust the orientation of the camera After making the appropriate adjustments tighten the screw Adjusting the camera orientation 3 Select the camera scene mode suitable for the microscopy type 4 Adjust the camera s white balance When using diascopic illumination first capture an image of the top surface of the...

Page 38: ...ography of the color fading portion requires a prolonged exposure time resulting in poor color reproduction and low quality images Move the sample so that an image of the portion that has not been exposed to excitation light can be captured To obtain the best results use the diascopic illumination method to select a specimen section for photomicrography and then switch to the fluorescent method to...

Page 39: ...25 Chapter 4 Functions and Operations of the Devices This chapter describes the main functions and procedure for using the devices ...

Page 40: ...or an epi fluorescence attachment between the zooming body and the tube SMZ800N Zooming Body Zoom images in or out using the zoom knobs on the right and left sides of the zooming body Turning the knob backward Magnification increase Turning the knob forward Magnification decrease Turning the knob causes the magnification to continuously change in the range from 1x to 8x zoom ratio 8 1 Magnificatio...

Page 41: ...ents via a USB Attach a tube to the tube mount round dovetail Microscope system functions can be expanded by assembling a device such as a coaxial epi illuminator or an epi fluorescence attachment between the zooming body and the tube SMZ1270 Zooming Body SMZ1270i Zooming Body SMZ1270i Zooming Body connectors Zoom in or out images using the zoom knobs on the right and left sides of the zooming bod...

Page 42: ...vating zoom click stops The SMZ1270i is used as an example If a hex wrench cannot be easily turned Loosen the zooming body fixing screw on the focus mount turn the zooming body to the right and left and then use the hex wrench if it cannot be used easily in the assembled state Disconnect the cables connected to the SMZ1270i Before enabling or disabling the zoom click stops on the SMZ1270i Zooming ...

Page 43: ... the focus knobs To adjust the torque of the focus knobs turn the right and left focus knobs in opposite directions Adjust the torque to an appropriate level Notes on the torque adjustment When the torque is too low the zooming body may slide down due to the various loads it carries such as its own weight or the load of the counterbalance spring inside the focus unit This may result in injury such...

Page 44: ... and left focus knobs in opposite directions Adjust the torque to an appropriate level Notes on the torque adjustment When the torque is too low the zooming body may slide down due to the various loads it carries such as its own weight or the load of the counterbalance spring inside the focus unit This may result in injury such as pinching fingers between the objective and sample or cause damage t...

Page 45: ...so may cause malfunction Do not turn the knobs in opposite directions Torque adjustment of the focus knobs To change the torque of the focus knobs turn the torque adjustment ring on the base of the left coarse focus knob using the tool supplied with the stand Adjust the torque to an appropriate level The hex driver is fitted on top of each support pillar of the P PS32 P DSL32 or P DSF32 Notes on t...

Page 46: ...ed in the middle of the support pillar be sure to attach the anti drop collar under the focus mount so that it fits Anti drop collar A C LSL2 LED Epi Illuminator 2 can be attached to the C FMCN Focus Mount via an SMZ U Epi Arm or a G EIA Flexible Arm A nosepiece can be used together with a zooming body combined with a C FMCN Focus Mount Anti drop collar ...

Page 47: ...nosepiece can be switched by turning the nosepiece Vertical observation with a single side optical path is also possible Stereoscopic view and vertical view The nosepiece has two click positions for each objective The view state can be changed between stereoscopic view and vertical view according to the click position Microscopy can be performed in the vertical position when needed such as when ta...

Page 48: ...e can be switched by turning the nosepiece Vertical observation with a single side optical path is also possible For details on vertical observation see the previous section 3 1 P RN2 Nosepiece Stereoscopic view and vertical view Objective address stereoscopic vertical view information is sent to the SMZ1270i Zooming Body via a nosepiece cable and then output to a DS L3 DS L4 camera control unit o...

Page 49: ...8 44 mm P ED Plan 2X WF 2x 0 21 35 mm P Plan Apo 0 5X WF 0 5x 0 053 82 5 mm P Plan Apo 0 75X WF 0 75x 0 079 107 mm P Plan Apo 1X WF 1x 0 105 70 mm Restriction P Achro 0 5X P ED Plan 0 75X and P Plan 1X cannot be used with SMZ1270 SMZ1270i Total magnification The total magnification of the microscope is determined from the formula below Total magnification zoom magnification x objective s magnifica...

Page 50: ...e support pillar Focusing stroke 100 mm Stroke per focus knob rotation 21 7 mm Stage plate size 90 mm dia Arm position change The arm can be attached to the position 55 mm lower than the standard See Chapter 5 3 1 Assembly of the Basic System 1 Install a stand C PSN C PSCN Plain Stand s arm position change C PSN Plain Stand Torque adjustment of the focus knobs To adjust the torque of the focus kno...

Page 51: ...cannot be used together with the microscope system that includes a C PSN C PSCN Plain Stand The C PSCN is a space saving model with a small base The C PSCN is not suitable for a top heavy system such as when a camera is attached to the tube 5 2 C DS Dia Illumination Stand This stand is equipped with a diascopic illumination function that uses a 6V20W halogen lamp or a C DSLU2 LED Unit for Dia Illu...

Page 52: ...ved horizontally and vertically For details refer to the instruction manual supplied with the stand 6 2 G US2 Universal Table Stand This stand is equipped with a base Attach a C FMBN Focus Mount to the arm that can be moved horizontally and vertically For details refer to the instruction manual supplied with the stand 6 3 US 3 Universal Stand This stand is equipped with a base Attach a C FMAN Focu...

Page 53: ...S32 Plain Stand Sliding mechanism of the stage plate The sliding parts are mounted on the base part for mounting the stage plate which can be slid by loosening the stage plate fixing screw There is a clearance gap of approximately 3 mm around the stage plate when the stage is securely set The stage plate can be smoothly and finely moved within the clearance gap by loosening the stage plate fixing ...

Page 54: ...er on the top surface of the base Usable for multiple purposes 4 4 M5 Positioned at the rear on the top surface of the base Used for attaching a C FDF Flexible Double Arm Fiber Illumination Unit via a C FIDH Fiber Holder Screw holes for attaching optional devices Exchange for a C EP support The support pillar length is 100 mm longer than standard when the original support pillar for this stand is ...

Page 55: ... Stand P SXY64 XY Stage XY stroke of the stage with the X Y shift knob and stroke per knob rotation X 150 mm 37 5 mm per rotation Y 65 mm from the optical path 50 mm towards the front 15 mm towards the rear 24 1 mm per rotation Load weight 5 kg or less 8 2 C SSL DIA Sliding Stage This stage is movable in the range of 19 mm in the radial direction by pressing the stage s side The height of the stag...

Page 56: ...ach a Circular Floating Stage 2 Usable stands C PSN C PSCN Plain Stand P PS32 Plain Stand Circular Floating Stage 2 8 4 C TRS Tilting Stage This stage can be tilted within 30 of the tilt range to observe samples The rotation center of the tilt is positioned in the center on the upper plane of the stage Attach the stage to the position where the stage plate was removed from the base Attach the remo...

Page 57: ...the binocular eyepiece tube is 20 Eye point height when used with 10x eyepieces Approx 121 mm from the mount joint interpupillary of 58 mm Field of view number 22 P B Binocular Tube Note on adjusting the interpupillary distance Do not apply excessive force to the binocular part during interpupillary distance adjustment Doing so may cause unexpected failure or malfunction Combination with the P IBS...

Page 58: ...inocular tube with 10x eyepieces attached Approx 48 mm from the mount joint Field of view number Binocular eyepiece tube 22 Vertical tube 18 Adjusting the depression angle of the trinocular tilting tube Notes on adjusting the depression angle and interpupillary distance Do not apply excessive force to the binocular part during depression angle or interpupillary distance adjustment Doing so may cau...

Page 59: ...ve Screw the plate in the tip of the corresponding objective The brightest illumination is generated when the yellow indication is at the front Select the position that has the optimum brightness and contrast for observation P CI Coaxial Epi Illuminator Color temperature compensation filter If the image appears yellowish during coaxial episcopic observation color shade can be adjusted by screwing ...

Page 60: ...centered around a single axis Z axis This allows the arms to be pushed aside without having to unlock the arm joints If the arm position is returned by rotating it on the same axis the arm will stop at the first lock position The C FIDH Fiber Holder can be attached to the following stands P PS32 Plain Stand P DSL32 LED Diascopic Illumination Stand P DSF32 Fiber Diascopic Illumination Stand C FIDH ...

Page 61: ... or apply excess force to the fibers Doing so may damage the fiber For details on attaching the unit to an objective connecting to a light source and using a light source refer to the instruction manual supplied with the light source C FIR Ring Fiber Illumination Unit 10 5 Other Episcopic Illuminators In addition to the illuminators with the episcopic illumination function described in Section 10 ...

Page 62: ...nce attachment Turn the turret using the filter cube switching knob Align a turret address number 1 to 4 displayed on the knob with the indicator to bring the filter cube of the selected address into the optical path INTENSILIGHT C HGFI LAMP ND 1 2 4 32 16 8 SHUT TER POWE R RUN TIME hrs P EFL Epi Fluorescence Attachment CAUTION Do not use the epi fluorescence attachment while the filter cube repla...

Page 63: ...useful for viewing the entire image of an opaque specimen during fluorescence observation Even though the P EFLBF Filter Cube has a built in UV cut filter adjust the light intensity to the appropriate level with the light source illuminator so that illumination is not too bright In addition two types of 1 4 λ plates are provided in order to prevent flares at the imaging system caused by the light ...

Page 64: ...used CTRL CTRL INPUT 12VDC 3 5A ON OFF Left side P DSL32 LED Diascopic Illumination Stand Power supply and illumination control Power to the P DSL32 LED Diascopic Illumination Stand is supplied by connecting a P2 CTLB Control Box or a 12 V AC adapter To provide diascopic illumination without using a control box connect a 12 V AC adapter Turn on or off the LED diascopic illumination using the LED p...

Page 65: ...nob position can be reproduced using the OCC adjustment knob with a scale Usable objectives and recommended zoom magnifications Objective Zoom magnification Bright field diascopic illumination OCC illumination 0 5X 1 5x 8x Contrast in the field of view is not uniform 0 75X 1x 8x Contrast in the field of view is not uniform 1X Min 8x 5x 8x 1 5X Min 8x 3x 8x 2X 2 5x 8x 1 5x 8x Shading may occur in t...

Page 66: ...ar The base with a large glass plate 180 mm dia enhances usability and enables easier observation of samples in a container such as a petri dish The base has three indents for holding the base on the right and left planes and the rear plane A dedicated hex driver is located at the top of the support pillar P DSF32 Fiber Diascopic Illumination Stand Sliding mechanism of the stage plate The sliding ...

Page 67: ...ver to the left For high magnification objective lens 2x Usable objectives and recommended zoom magnifications Objective Zoom magnification Bright field diascopic illumination OCC illumination 0 5X condenser lens for low magnification 1 5x 8x Unusable 0 75X condenser lens for low magnification 1x 8x Unusable 1X condenser lens for low magnification Min 8x 3x 8x 1 5X condenser lens for low magnifica...

Page 68: ...t of the base Used for attaching the P SXY64 XY Stage 3 2 M4 Positioned at the center on the top surface of the base Usable for multiple purposes 4 4 M5 Positioned at the rear on the top surface of the base Used for attaching a C FDF Flexible Double Arm Fiber Illumination Unit via a C FIDH Fiber Holder Screw holes for attaching optional devices Exchange for a C EP support The support pillar length...

Page 69: ... is used the periphery becomes dark in the low zoom magnification range To reduce vignetting remove the stage glass and directly place the polarizer Drop the polarizer removed from the polarizer section into the position where the diffuser was removed from the base of the stand Use the original stage plate supplied with the base The polarizer cannot be placed directly on the C DS Dia Illumination ...

Page 70: ...software NIS Elements to the control box in order to control illumination For details on the procedure for controlling illumination refer to the instruction manual for NIS Elements P2 CTLB Control Box Rear of the P2 CTLB Use the power cable specified in Chapter 8 2 Performance Properties to connect to the AC adapter USB Connected to a PC LEDDIA Connected to the LED light control terminal of the P ...

Page 71: ...r sizes that vary between 16 mm dia and 6 5 mm dia SMZ10A Aperture Unit 14 2 P IBSS2 Beam Splitter Attach the P IBSS2 between the zooming body and the tube to split the optical path Mounting a digital camera on the beam splitter port via a camera adapter enables image capture of the specimen being observed Also read the instruction manual supplied with the digital camera in use P IBSS2 Beam Splitt...

Page 72: ...THSS Teaching Head Observation by main and secondary observers is enabled by attaching a zooming body and a tube Simultaneous observation of the same viewfield is possible through the eyepieces on the secondary observer side and the eyepieces on the main observer side making it ideal for educational purposes For details refer to the instruction manual supplied with the P THSS Teaching Head 14 5 P ...

Page 73: ...depression at the front of the base and the rear of both sides to lift up the system Be very careful not to let fingers be caught when putting it down Tools necessary for assembly Tools Devices that supply tools Hex wrench nominal designation 2 SMZ800N SMZ1270 SMZ1270i Zooming Body Hex wrench nominal designation 2 5 P SXY64 XY Stage Hex wrench nominal designation 3 P SXY64 XY Stage Hex wrench nomi...

Page 74: ... Illuminator via an HG fiber P EFL Epi Fluorescence Attachment Note 1 with a shielding plate Objective 1 4 λ plate USB cable P B Binocular Tube C FLED2 LED Light source for fiber illuminator via a fiber SMZ1270 Zooming Body SMZ1270i Zooming Body P RN2 Nosepiece Note 2 P RNI2 Intelligent Nosepiece Note 2 C FMBN Focus Mount C FMCN Focus Mount C PSN C PSCN Plain Stand C DS Dia Illumination Stand C LE...

Page 75: ...ED Stand C LSL2 LED Epi Illuminator 2 SMZ U Epi Arm G EIA Flexible Arm C FDF Flexible Double Arm Fiber Illumination Unit C FIR Ring Fiber Illumination Unit Objective P ED Plan 2X WF cannot be used C FIDH Fiber Holder C FID2 Double Arm Fiber Illuminator C FLED2 LED Light source for fiber illuminator P DF LSP Light Shielding Plate P DF2 LED Dark Field Unit 2 Stage adapters for AZ P SXY64 XY Stage C ...

Page 76: ...on Stand C DS Hybrid LED Stand C LEDS Universal Table Clamp Stand G US1A Universal Table Stand G US2 Universal Stand US 3 XY Stage P SXY64 Optional Stage adapters for AZ Various types Optional DIA Sliding Stage C SSL Optional Circular Floating Stage 2 Optional Tilting Stage C TRS Optional LED Dark Field Unit 2 P DF2 Optional Light Shielding Plate P DF LSP Optional Simple Polarizing Attachment C PO...

Page 77: ...ered Fiber Illuminator C HGFI Optional Light source for P EFL HG Precentered Fiber Illuminator C GHFIE Optional Light source for P EFL LED Light source for fiber illuminator C FLED2 Optional Light source for P CI P DSF32 C FDF C FID2 and C FIR LED Unit for Dia Illumination Stand 2 C DSLU2 Optional Light source for C DS Control Box Control Box P2 CTLB Optional Power supply when not connecting an AC...

Page 78: ... the stand P PS32 Plain Stand P DSL32 LED Diascopic Illumination Stand P DSF32 Fiber Diascopic Illumination Stand Attach a support pillar supplied with the stand to the stand The procedure for attaching the support pillar is the same for all stand types Insert the support pillar to the stand and tighten the clamp screw on the rear side using the hex driver supplied with the support pillar The hex ...

Page 79: ...h the stand to the base Insert the stage plate tilted toward the front and push in the rear side while holding the front side The stage plate is securely attached by the spring Instead of the standard stage plate an antistatic plate ESD stage plate can be attached by following the same procedure Attaching the stage plate P DSL32 LED Diascopic Illumination Stand cable connection Connection of the p...

Page 80: ...screw hole at the right rear side of the base and loosen the internal fixing screw M4 set screw Insert the fiber to the rear side of the base until it reaches the limit and tighten the fixing screw Firmly insert the other end of the fiber into the fiber attachment hole of the C FLED2 LED Light Source for Fiber Illuminator See the instruction manual for the light source for details Securely attachi...

Page 81: ...Do not attach the focus mount if the focus mount does not face the front of the stand as shown in the figure Incorrect focus mount orientation Focus mount attachment position 1 Standard position Insert the focus mount until it reaches the lower limit of the support pillar and tighten the focus mount fixing knob on the rear side Securely attaching the focus mount 2 Position higher than the standard...

Page 82: ... Dia Illumination Stand and C LEDS Hybrid LED Stand is equipped with a vertically movable arm The focus mount is not required to be attached 3 Attach a zooming body Attach a zooming body to the focus mount or to the vertically movable arm of the stand The procedure for attaching a zooming body is the same for all types Loosen the zooming body fixing screw of the focus mount or vertically movable a...

Page 83: ...d dovetail at the bottom of the tube with the positioning pin on the rear of the round dovetail groove at the zooming body upper plane so that they fit and then tighten the fixing screw SMZ8 00 Securely attaching the tube 5 Attach eyepieces Attach eyepieces to the tube Use two eyepieces of the same magnification Completely insert them into the tube until they touch the edge plane of the tube sleev...

Page 84: ... types Screw the objective into the objective attachment screw hole at the lower plane of the zooming body Insert it straight from the bottom and securely screw it until it touches the abutting joint Attaching the objective Restriction P Achro 0 5X P ED Plan 0 75X and P Plan 1X cannot be used with the SMZ1270 SMZ1270i The basic system assembly is complete ...

Page 85: ...he nosepiece is in the stereoscopic view state as shown in the figure Nosepiece in the stereoscopic view state The P RN2 is used as an example Note that the nosepiece in the vertical view state as shown in the figure cannot be attached Nosepiece in the vertical view state Attachment not possible in this state 2 Align the positioning pin for attaching the nosepiece with the positioning groove at th...

Page 86: ...f the round dovetail at the bottom of the coaxial epi illuminator with the positioning pin on the rear of the round dovetail groove at the zooming body upper plane so that they fit and then tighten the fixing screw Securing attaching the coaxial epi illuminator Fiber attachment First loosen the M4 set screws for the fiber on the side plane of the two fiber attachment holes on the rear side of the ...

Page 87: ...G fiber attachment Loosen the M4 set screw for the HG fiber on the top of the HG fiber attachment hole on the rear side of the epi fluorescence attachment using a hex wrench nominal designation 2 Insert the emission side tip of the HG fiber to the attachment hole until it reaches the limit and tighten the fixing screw Firmly insert the source side connector to the HG fiber attachment hole of the C...

Page 88: ...filter to the plain label when using the P EFLC Label RFP The supplied filter cube labels used in common with other devices include a label RFP that is not used for the P EFL Epi Fluorescence Attachment Do not use the label RFP P2 EFLC MADE IN JA PAN 4 1 1 2 3 4 4 Affixing a label indicating the type of the filter cube 4 Return the cover of the filter cube replacing hole to the original position 1...

Page 89: ...osen and remove the two top part fixing screws from the top surface of the filter cube to take off the top part 2 Loosen and remove the four cover fixing screws from the right and left sides of the filter cube to take off the cover Entire P EFLC Top part removed Cover removed bottom part P EFLC disassembly See the section view on the right for the reference plane of each part Reference plane 2 Bon...

Page 90: ...turer s instructions for the orientation of the excitation filter Excitation filter setting 2 Fill in the four bonding positions of the bottom part with adhesive to bond the excitation filter to the bottom part Excitation filter setting 5 Bond to attach the dichroic mirror 1 Place the dichroic mirror so that its evaporated surface contacts the bonding surface and the side touches the abutting surf...

Page 91: ...iber Holder to the right and left rear sides of the base Two bolt holes are located on each side Tighten four M5 hexagon socket head bolts using a hex wrench nominal designation 4 Securely attaching the fiber holder 2 Insert the flexible double arm fiber tip into the ring at the arm end and tighten the M4 set screw for the fiber using a hex driver nominal designation 2 3 Firmly insert the fiber so...

Page 92: ...the stage plate fixing screw for the C SSL DIA Sliding Stage Securely attaching the C SSL DIA Sliding Stage 8 Attach a Circular Floating Stage 2 Attach a Circular Floating Stage 2 to a P PS32 Plain Stand C PSN Plain Stand or C PSCN Compact Plain Stand 1 Remove the stage plate from the base 2 Place a Circular Floating Stage 2 so that it fits the base and then securely attach it by tightening two M2...

Page 93: ...olarizer section into the position on the base or the stand where the diffuser was removed Use the original stage plate supplied with the base The polarizer cannot be placed directly on the C DS Dia Illumination Stand 10 Attach a P SXY64 XY Stage Attach an XY stage to a P PS32 a P DSL32 or a P DSF32 stand 1 Remove the fastener for the stage Loosen the four M4 hexagon socket head bolts for the stag...

Page 94: ... screw on the aperture unit to securely attach the tube SMZ8 00 Securely attaching the aperture unit Using together with a coaxial epi illuminator When using the SMZ10A Aperture Unit together with the coaxial epi illuminator first attach the SMZ10A Aperture Unit and then attach the coaxial epi illuminator on top 12 Attach a P IBSS2 Beam Splitter Attach the P IBSS2 Beam Splitter after performing 3 ...

Page 95: ... previous section 3 1 step 4 Tighten the tube fixing screw of the eyelevel riser to fix the tube in place Securely attaching the eyelevel riser 14 Attach a camera 1 Screw the C mount lens in the DS camera attach the DS camera head to the LV TV TV Tube Adapter and then tighten the camera attachment direction fixing screw on the TV tube adapter 2 Loosen the TV tube adapter fixing screw located on th...

Page 96: ...Chapter 5 Assembly 82 ...

Page 97: ...erly If any of the problems described in this chapter occur be sure to check the table for possible causes before requesting service If problems that are not listed in the table are detected or the problem still persists even after the indicated measures are taken turn off the device and contact your nearest Nikon representative ...

Page 98: ...2 Operation Problem Cause Measure Even when looking with both eyes an image does not appear as a single image The interpupillary distance has not been adjusted Adjust the interpupillary distance The diopter has not been adjusted Adjust the diopter distance Eyes become fatigued The interpupillary distance has not been adjusted Adjust the interpupillary distance The diopter has not been adjusted Adj...

Page 99: ...85 Chapter 7 Maintenance and Storage This chapter describes how to maintain and store the product ...

Page 100: ...ing of the printed characters 1 1 Cleaning Lenses Keep the lens free of dust and fingerprints Any contamination on the lenses or filters will reduce the image quality If the lenses have become dirty clean them as follows Cleaning light dirt dust 1 Use an air blower to blow off any dust 2 If any dust remains brush the dust off using a soft brush or gently wipe the dust off using a piece of gauze Cl...

Page 101: ...stablished for your facility 2 Storage Store this product in a dry location free of mold or mildew The storage conditions are as follows temperature 20 C to 60 C humidity 90 RH max no condensation Place a cover over this product to protect it from dust Switch off the device press the switch to the O position If the device is warm wait for it to cool before covering it with a cover 3 Periodic Inspe...

Page 102: ...Chapter 7 Maintenance and Storage 88 ...

Page 103: ...89 Chapter 8 Specifications ...

Page 104: ... accuracy of the numeric information such as magnifications or Z position information is not guaranteed These values are used as a reference and include a margin of error The product is classified as an in vitro diagnostic medical device IVD Intended user It is intended for the researchers medical professional and those who work on experiments in the field of pathology and cytology 2 Performance P...

Page 105: ...on Stand Others Anti drop collar supplied Stand Name Plain Stand Model C PSN C PSCN Focusing mechanism With single axis focus knobs on the support pillar Focusing stroke 100 mm Stroke per focus knob rotation 21 7 mm rev Knob torque adjustable Screw hole Screw holes for optional devices Stage plate Black surface and milky white surface acrylic 90 mm dia Attached to the base using a leaf spring Exch...

Page 106: ...hing type Filter NCB11 ND4 ND16 Removable Light source C FLED2 LED Light Source for Fiber Illuminator Refer to the instruction manual for the light source Focusing mechanism None Support pillar vertical movement system using the focus dial on the base Focusing stroke 6 2 mm Stroke per focus dial rotation 3 24 mm Support pillar vertical movement system using the focus dial on the base Focusing stro...

Page 107: ...SN C PSCN Plain Stand a C DS Dia Illumination Stand or a C LEDS Hybrid LED Stand Nosepiece Name Nosepiece Intelligent Nosepiece Model P RN2 P RNI2 Function A double hole nosepiece that can hold two objectives so that the objectives are switched during observation The stereoscopic view and vertical view can be generated by shifting the objective position Attached to the objective mount of the zoomi...

Page 108: ...cation 1 5x Episcopic illumination Episcopic illumination through a two branch fiber Light source C FLED2 LED Light Source for Fiber Illuminator Refer to the instruction manual for the light source Episcopic illumination through fiber branched into two Light source C FLED2 LED Light Source for Fiber Illuminator Refer to the instruction manual for the light source Used together with a C FIDH Fiber ...

Page 109: ...N2 C AC AC Adapter Model name by the manufacturer PW 120A2 1Y240G Manufacturer FSP Group Inc POWER WIN TECHNOLOGY CORP Input rating 100 240 VAC 10 1 5 A 50 60 Hz 100 240 VAC 10 2 1 A 50 60 Hz Output rating 12 VDC 3 33 A Max 24 VDC 5 0 A Max Power cable Relevant device P2 CTLB Control Box P DSL32 LED Diascopic Illumination Stand 12 V AC adapter 3 AC Model name by the manufacturer FSP040 RHAN2 24 V ...

Page 110: ...s equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense CAN ICES 003 A NMB 003 A Austr...

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