Nikon Optiphot, Quick Reference Manual

Page: 7 / 7

Digital Microscopy Center, University of Washington
July 29, 2016 7
Table 3. Epi-fluorescence filter choices
*UV capable, coverslip correction collar
**Variable NA 0.8-1.3
Table 4. Spatial calibration for each objective lens, with the Spot IIe camera.
Objective lens notes.
Other objective lenses are available upon request, such as 60/1.4 oil, 100/1.25 oil.
Lenses designated as “160/-” do not require a coverslip.
Lenses designated as “160/.17” require a coverslip of 170
µ m thickness.
Filter Label Cube Label “Color” Part Nikon
FITC HQ:FITC green 96170 C01174
TxR HQ:TXRD red 96172 C00178
UV UV-2E/C blue blue
Cy5 Cy5 far red 31023 C81736
Lens µm/pixel pixel/µ
m
1 mm reticle
field
1/.04 Plan 160/- 8.40 0.12
2/.05 Plan 160/- 4.24 0.24
4/.1 EPlan 160/- 2.12 0.47
10/.40 EPlan 160/- 0.87 1.15 820 µm
2
20/.40 EPlan 160/- 0.43 2.31 412 µm
2
40/.65 EPlan 160/.17 0.21 4.78 200 µm
2
40/.85 Fluor 160/.17* 0.21 4.78 200 µm
2
100/1.30 Oil Fluor 160/.17** 0.09 11.55