Chapter 1
Terminology Used in This Document
3
Continuous mode
Method of acquiring STORM images using a monomolecular dye that can itself become bright or dark, instead of requiring a
dye pair for activation. Since the activation and imaging laser light are emitted simultaneously to acquire images, acquisition
takes less time than in normal mode. In this case, the definitions of cycle and period are not applied.
Dataset structure in continuous mode (example)
Non-specific activation (NSA)
During image acquisition in normal mode, only those molecules that are detected in the first imaging frame after activation
are classified as being of the relevant channel. Also, those molecules that are not detected in the first imaging frame but in
the second or subsequent frames are classified as being those of a non-specific activation (NSA) channel. Information on
non-specific activation channels is used for crosstalk subtraction. (For details, see step 6 in “2.3 N-STORM Analysis,” in
Chapter 2.)
Activation
laser (405 nm)
Imaging
laser (647 nm)
ON
ON
ON
ON
ON
ON
ON
ON
Frame
Dataset
ON
ON
ON
ON
ON
ON
ON
ON
(Repeat the same
operation.)