Chapter 2
STORM Microscopy Operation
26
6
Subtract crosstalk. (Only in normal mode. The
crosstalk subtraction function is not used for images
acquired in continuous mode.)
(1) Click [Cross-Talk Subtraction].
(2) For [Source Channel (Ch A)], select the channel for
which crosstalk is to be subtracted.
(3) Select the method and the threshold for crosstalk
subtraction. Use the [Threshold] slider or input a
value to specify the threshold.
When specifying the method and the threshold for crosstalk subtraction, refer to the table below.
Method of crosstalk
subtraction
Recommended uses
Destination of the
molecules exceeding
the threshold
(subtracted
molecules)
Recommended
threshold (ratio of
surrounding
molecules)
Ch A
–
Ch B
If the result of imaging is
predicted (Ch A and Ch B are
found to be in clearly separate
areas). (Can be used for
2-channel operation only.)
Ch B
0.51
Ch A
–
NSA
If the result of imaging is not
predicted.
NSA
0.49
Ch A
–
(Ch B + NSA)
If the result of imaging is not
predicted.
(Can be used for 2-channel
operation only.)
NSA
0.49
If Ch A – NSA is selected, information on the molecules of Ch B is not used. Molecules that are regarded as
NSA by referring to the NSA frames (the second and third imaging frames of Ch A) are removed.
6-(3)
6-(2)
6-(4)
