Chapter 1
Terminology Used in This Document
2
Imaging frame
Frame (image) that is acquired when laser light for imaging is emitted. In this frame, fluorescent probe molecules fluoresce
only when activation has caused them to become activated. Note that if images are to be acquired in continuous mode, there
is no difference between the activation and imaging frames because activation and imaging are performed simultaneously.
Cycle
Set of frames that consist of an activation frame (normally one frame) and the subsequent imaging frames (normally three
frames) on one channel during image acquisition in normal mode.
Channel
For a multistaining procedure, the cycle in which each dye is observed is called a channel.
Period
Set of cycles that are made up of one cycle of each of the multiple channels on which images are acquired in normal mode.
For example, if images are acquired on two channels, one period consists of two cycles. If images are acquired on only one
channel, a period is the same as a cycle.
Dataset structure in normal mode (example)
Number of image acquisition channels: 2
Number of activation frames per period (per cycle) on one channel: 1
Number of imaging frames per period (per cycle) on one channel: 3
A1:
Activation frame on channel 1
I1:
Imaging frame on channel 1
A2:
Activation frame on channel 2
I2:
Imaging frame on channel 2
Activation laser
Channel 1 (405 nm)
Activation laser
Channel 2 (561 nm)
Imaging
laser (647 nm)
ON
ON
ON
ON
ON
ON
ON
ON
ON
ON
ON
Frame
Cycle
Cycle
Period
Dataset
(Repeat the same
operation.)
A1
I1
I1
I1
A2
I2
I2
I2
A1
I1
I1
