Chapter 3 Usage of Components
72
3.13 Using the Epi-fluorescence Attachment
3.13.1 Adjusting
the
Aperture
Diaphragm of the Epi-fluorescence Attachment
The aperture diaphragm is used to adjust the
number of illumination apertures. In an optical
system for the epi-fluorescence attachment, the
aperture diaphragm adjusts the brightness of the
image and the amount of stray light.
The epi-fluorescence attachment modules are
equipped with an aperture diaphragm open/close
lever and a centering knob, which enable the user to
adjust the aperture diaphragm.
Operation:
Aperture diaphragm
open/close lever*
Aperture diaphragm
Turn it to the lower side.
The diameter of the
diaphragm increases.
Turn it to the upper side.
The diameter of the
diaphragm decreases.
The narrower the aperture diaphragm is, the
smaller, the amount of stray light becomes, but the
darker the image of the specimen becomes. The
wider the aperture diaphragm is, the brighter, the
image becomes, but the larger the amount of stray
light becomes.
By narrowing the aperture diaphragm to the size
equal to the pupil diameter of the objective,
brightness of the specimen image can be kept and
stray light can be removed effectively.
Aperture diaphragm open/close lever (EPI-FL module)
Using the aperture diaphragm open/close lever
The label shown on the right is affixed next to the aperture
diaphragm open/close lever.
Pay attention to the orientation of the label when adjusting the
aperture diaphragm of the epi-fluorescence attachment.
A.S.
Aperture diaphragm
centering knob
(top and bottom)
Aperture diaphragm
open/close lever
* When the main branch is used in an inverted layout,
the direction of operation is the opposite.
