6
Iris diaphragm should not be used to control the brightness of illumination. Iris diaphragms are designed to
help achieve high resolution of specimen and provide contrast in the image. Smaller apertures will deliver
higher contrast to image. However, closing aperture too much will reduce resolution. Experimentation is the
best method of determining the correct opening of diaphragm. Some suggested openings for iris diaphragm
are:
OBJECTIVE
DIAPHRAGM OPENING
4x
From fully closed to 1/8 open
10x
1/8 to 1/4 open
40x
1/4 to 1/2 open
100x
1/2 to 3/4 open
G. Changing
magnification.
a) Rotate revolving nosepiece to position 10x objective into optical path.
b) This microscope has been parfocalized, which allows changes from one objective to another while
requiring only a slight adjustment of the fine focus controls.
c) When changing to the 40x and 100x objective lens, care must be exercised in order to prevent damaging
the front lens element and specimen slide.
d) In order to obtain maximum resolution of the 100x oil immersion lens, it is necessary to apply immersion
oil between the coverglass of slide and front lens of the objective.
(1) Use of a very small amount of immersion oil is required.
(2) All air bubbles must be removed from between lens and slide by rotating nosepiece back and forth.
MICROSCOPE SPECIFICATION CHART
EYEPIECE OBJECTIVE
MAGNIFICATION
FIELD
SIZE WORKING
DISTANCE
4X 40X
4.5MM
10MM
10X 100X
1.8MM
2.4MM
40X 400X
0.45MM
0.48MM
WF10X
100X 1000X
0.18MM
0.04MM
MONITOR MAGNIFICATION
MAGNIFICATION as viewed is dependent on YOUR MONITOR and other variable factors. Factors
determining magnification are capture screen size, viewable size of monitor screen, horizontal and vertical
size controls and zoom control of monitor. The built-in camera has its own CCD lens and is totally
independent of eyepieces, changing to eyepieces of different magnification will not effect image magnification,
nor will reticles installed in eyepieces be viewed on monitor screen.
MONITOR FIELD SIZE
Approximately 90% of what you view through the eyepieces of the microscope can be viewed on your monitor.
Field size is not significantly affected by the factors that change monitor magnification.
H. When finished viewing, all parts that come in contact with oil must be cleaned. Failure to do so could
permanently damage the 100x oil immersion objective lens. Use of xylene to clean immersion oil off lens
surfaces is recommended.
