10
Setting
Parameter
2. The parameters are described below:
3. Press NEW button to create a new protocol.
[Sensitivity]
Sensitivity (refers to the contrast of the objects against the background).
Adjusting the sensitivity higher makes instrument more sensitive to ob-
jects; useful for cells that do not stain well with trypan blue while adjust-
ing the sensitivity lower makes the instrument less sensitive and is useful if
there is a lot of background.
[Minimum cell size]
Minimum cell size is used to determine the low range of cell size to include
in the measurement. The algorithm first identifies all objects, and calcu-
lates the average size (e.g., 15 µm). From the percent of average size set-
ting, the algorithm calculates the smallest object size to include in the final
measurement (e.g., 70% of 15 is 10.5 µm; 15–10.5 = 4.5 µm; 4.5
µm would be the smallest particle included in the count). Adjusting the
number up, increases inclusiveness thereby decreasing the lower cell size
range (e.g., 50% of 15 is 7.5 µm; 15–7.5 = 7.5 µm).
Maximum cell size is used to determine the high range of cell size to in-
clude in the measurement. The algorithm first identifies all objects, then
calculates the average size (e.g., 15 µm). From the percent of average size
setting, the algorithm calculates the largest object size to include in the
final measurement (e.g., 200%; 200% of 15 µm = 30 µm; 30 µm is the largest
cell size included in the measurement.
Circularity is used to determine the objects to include in the measurement
based on roundness. Increasing the value from 80% requires objects to be
more round for inclusion in the measurement. Decreasing the value from
80% allows objects to be less round. Adjusting this may be useful if the cell
type is not particularly circular or perhaps oddly shaped.
After modifying any parameters,
press APPLY button to make the changes.
To restore default parameters, press DEFAULT button.
4Ch
