20
1.
Cultivate the required number of cells.
2.
Add an appropriate volume of growth media or PBS to dilute
to a final concentration of 5x10
4
cells/mL to 4x10
6
cells/mL.
☞
Note :
Concentration out of this range will result in errors.
Refer to page 23 for more information about errors.
3.
Thoroughly mix the cell pellet by vortexing.
4.
Check visually if any cell clumps or agglomerates are remained.
General operation
Introduction
Preparing
cell
Counting cell
Instruction is provided in this section for preparing the cell
sample with AccuStain solution for use with disposable
AccuChip for automated cell count using the ADAM-MC.
• AccuChip 2x
• AccuChip 4x
Total Cell
1.
Add 50 μL of your sample to 50 μL supplied
AccuStain solution
T
.
2.
Mix gently by pipetting up and down.
3.
Load
20 μL
sample mixture to the AccuChipon
T
channel.
Total Cell
1.
Add 50μL of your sample to 50μL supplied
AccuStain solution
T.
2.
Mix gently by pipetting up and down.
3.
Load 13μL sample mixture to the AccuChip
on
T1
or
T2
channel.
Non-viable Cell
1.
Add 50 μL of your sample to 50 μL supplied
AccuStain solution
N.
2.
Mix gently by pipetting up and down.
3.
Load
20 μL
sample mixture to the AccuChipon
N
channel.
Non-viable Cell
1.
Add 50μL of your sample to 50μL supplied
AccuStain solution N.
2.
Mix gently by pipetting up and down.
3.
Load 13μL sample mixture to the AccuChip
on
N1
or
N2
channel.
Non-Viable
Non-viable
Non-viable
Total
Total
Total
