NanoEnTek ADAM CellT User Manual Download

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Instruction Manual

A New Standard of Automated Cell Counter

ADAM

Summary of Contents for ADAM CellT

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Page 3: ...ro Paltan myeon Hwaseong si Gyeonggi do 18531 Korea Tel 82 2 6220 7940 Fax 82 2 6220 7999 NanoEntek America Inc 220 Bear Hill Road Suite 102 Waltham MA 02451 USA Tel 1 781 472 2558 Fax 1 781 790 5649...

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Page 5: ...st Safety precautions Safety symbols Warnings Technical Support General Operation Introduction Sample preparation Counting cell Measure Run sample Result analysis Result analysis Error code Data QC mo...

Page 6: ...6 Introduction General Description The ADAM CellT is a benchtop automated cell counter designed to perform cell counting and viability measurements using AccuStain Solution...

Page 7: ...ddress these problems NanoEntek has developed the ADAM CellT which is based on a fluorescent microscopy technique for counting cells The ADAM CellT utilizes sensitive fluorescence dye staining LED opt...

Page 8: ...stained AO included in Solution TAO is permeable dye which permeates cell membrane and stains DNA Regardless of the condition of the cell membrane or active metabolism AO can stain both viable cells a...

Page 9: ...t room temperature AccuStain Solution should be stored at 2 8 C Item Quantity Main device 1 Power cord 1 USB hub 1 Adapter 1 User s manual 1 Support for 21 CFR Part 11 Compliance 1 AccuChip Kit 1 USB...

Page 10: ...cted Power Power on off LCD Display processes and results START Performs all procedures of automatic counting LOCK Protects the alignment of stage from external shock when ADAM CellT is being moved Lo...

Page 11: ...r view of ADAM CellT is shown below Port Description USB Port Port for software update and save the data PC port Connects with PC Power Plug Connects ADAM CellT power cord to wall outlet Product Descr...

Page 12: ...7 Ensure minimal contact with dust or other airborne particles 8 Allow a 10 cm 4 inches minimum space around the device for proper airflow 9 Do not place any objects on the device 1 Check the connecti...

Page 13: ...lure of X axis sensor 0x00007000 Failure of Y axis sensor 0x00008000 Failure of Z axis sensor 0x06000000 Failure of Locking module sensor Getting Started It appears when booting is not working properl...

Page 14: ...e Set the minimum and maximum size of cell Dilution factor When diluting sample set the Dilution factor Factor values for the AccuStain Solution is already applied CAUTION 2Ch 4Ch Accuchip 4Ch 2Ch Acc...

Page 15: ...mated cell count using the ADAM CellT Please check the procedure of sample preparation and testing below For more detailed information please refer to the next page 2 Load the mixed sample Then wait 1...

Page 16: ...Solution N 2 Voltex the tube vigorously 3 Load 23 L sample mixture to the AccuChip on N channel Then wait 1 minute for the sample settling Total Cell Non viable Cell 1 Add 50 L of your sample to 50 L...

Page 17: ...2 T1 N1 T2 N2 General Operation Counting cell Correct volume Low volume Sample loading error Be cautious of loading the correct volume of the sample into AccuChip The instrument will not detect low or...

Page 18: ...The instrument will not detect if slides are inserted incorrectly See pictures below for proper insertion WARNING PleaseinsertorremovetheAccuChipwhentheslideholderisfullyejected Whenthetestisfinished...

Page 19: ...d Cell Counter 19 Introduction Measure Run Sample Start counting process by pressing START It may take about 2 minutes longer for auto focus at the initial test While the test is in progress you can c...

Page 20: ...5 75 The viability will be automatically calculated by the ADAM CellT software after each measurement of the total cells and the non viable cells First the total cell number and second non viable cell...

Page 21: ...meter is larger than 100 m When this error shown in result window please check the image Error code Cause E Frames with errors are over 50 of total counting frame O Cells are more than 2X107 cells mL...

Page 22: ...Show Data Displays the date of QC progress on the graph QC Result Graph Graph left right QC date number movement button Arrow Button Graph left right QC date number movement button QC Slide QC Slide L...

Page 23: ...tered QC Slide lot information list New Registers new QC Slide lot Edit Edits selected QC Slide lot Delete Deletes selected QC Slide lot Apply Close Applies function or closes selected QC Slide lot Lo...

Page 24: ...ll Selects all QC result to delete from the QC result List QC Result List QC result information list Visible Selects all QC result to display graph from the QC result List Delete Deletes selected QC r...

Page 25: ...e different for each QC Slide lot and the acceptance slide peak size 13 16 um The acceptance range of the selected QC slide lot is displayed on the graph as a purple area Acceptance slide peak size re...

Page 26: ...edit the data Multiple data can be edited with the same settings IMAGE Allows to check the cell images of each channel SAVE Saves the selected data to USB PRINT optional Prints the selected data MAIL...

Page 27: ...w the cell size graph each channel T N Cell size table Allows to view the number of cells in each cell size Channel Select a channel T N Cell size setting Set the min max size of the cell Dilution Fac...

Page 28: ...AGE Control buttons Description Channel Select a channel Original Check the original image Counted Check the counted cell image Frame Select a frame number of the channel Zoom in out Zoom in and out t...

Page 29: ...oduction Data SAVE Control buttons Description Select Path Selects a save path from the list to send the selected data Data Type Selects which data type to save Apply Exports the files to a selected s...

Page 30: ...to send data The e mail address where data has been sent will be saved Mail Address To send files to new e mail enter the applicable e mail address Data Type Selects which data type to send via e mai...

Page 31: ...approval cannot be edited or deleted Approved data cannot be edited NOTE Approval Approval Control buttons Description Approval Status Settings tab related to data approval such as Requestable Reques...

Page 32: ...Approvable and Approved Data List List of data that can check status information related to approval All Select all data in Data List Search Display the data of the selected date Cancel Cancel approv...

Page 33: ...proval such as Requestable Requesting Approvable and Approved Data List List of data that can check status information related to approval All Select all data in Data List Search Display the data of t...

Page 34: ...al such as Requestable Requesting Approvable and Approved Data List List of data that can check status information related to approval All Select all data in Data List Search Display the data of the s...

Page 35: ...mote support software Mail DO NOT change the setting in mail Number Selects auto numbering Update Updates firmware or software through USB Auto Logout Sets auto logout time Date Time Sets current date...

Page 36: ...lick Remote support button 3 Share your ID and password to NanoEntek If connection error occurs please contact a laboratory facility manager CAUTION The remote support feature is to be used for mainte...

Page 37: ...repare the USB with update file 2 Insert the USB 3 Click the UPDATE button The AdamUpdate foldermustexistintherootpathoftheUSBfolder ADAM CellTcanbeupdatedonlywhenthefirmwareorsoftwarefileexistsin the...

Page 38: ...ckup Sync Manual recovery restores manually backup counted data Setting The automatic backup function is turned off after recovery please re enable the automatic backup function NOTE Please be careful...

Page 39: ...To logout User manage To register user Log manage Tracks user access records Document manage Tracks management document records Deleted list Tracks deleted data records ADAM CellTprovidesacomprehensi...

Page 40: ...iption User Name To view the registered user list Permission To view the user access authority New Registration To register new user Privilege Option for permission setting Supervisor Permission To re...

Page 41: ...ings Function list Detailed functions for each permission setting tab Detailed function List of possible permission settings for each function The Admin can grant or release access to functions when c...

Page 42: ...ount lock Number of incorrect passwords in account lockout 3 5 10 15 Minimum length Minimum length of password 3 5 10 15 Reuse Prohibition of using the same password for a certain period of time 30 90...

Page 43: ...l Counter 43 Introduction User Log manage Control buttons Description All Select all data in Data List SEARCH Display the data of the selected date SAVE Save the selected data to USB DELETE Delete the...

Page 44: ...ol buttons Description All Select all data in Data List SEARCH Display the data of the selected date SAVE Save the selected data to USB DELETE Delete the selected data CLOSE Close the document manage...

Page 45: ...Counter 45 Introduction Control buttons Description All Select all data in Data List SEARCH Display the data of the selected date SAVE Save the selected data to USB DELETE Delete the selected data CLO...

Page 46: ...on for 3 minutes the lock function will be activated automatically When the device is locked the screen will be changed as shown below If you press the power button for 2 3 seconds then Slide to shut...

Page 47: ...llT has no replacement of consumable materials 3 Please clean the exposed surface of ADAM CellT frequently or before testing using a soft cloth and isopropyl alcohol or deionized water Dispose of wipe...

Page 48: ...ion on each error message see page 21 When frames with errors are over 50 of total counting frame Error message E Check the suspension of cells if all cells are fully dissociated into single cells If...

Page 49: ...rts supplied by anyone other than NanoEntek 5 Damage caused by accident or misuse 6 Damage caused by disaster 7 Corrosion caused by improper solvent or sample For your protection items being returned...

Page 50: ...AO PI 4x105 2x106 cells mL PI 4x105 1x107 cells mL AO PI 25 50 sec test DC12V 5A 4 X 4W Green LED CMOS camera Excitation filter Dichroic filter Emission filter 7 Kg 227 276 270 mm IPX0 23 L test Accu...

Page 51: ...uchip 4x Kit AO PI 200 pcs AccuChip 4X 1 12 5 mL AccuStain Solution T TAO 2 12 5 mL AccuStain Solution N 1 ADR 1000 Accustain Solution PI solution 12 5 mL AccuStain Solution T TPI 4 12 5 mL AccuStain...

Page 52: ...objects especially metallic into the air vents of the instru ment as this could result in electrical shock personal injury and equip ment damage Always set the main switch on the power supply unit to...

Page 53: ...quirements Instruments bearing the TUV symbol are certi fied by TUV SUD America Inc to be in conformance with the applicable safety standard for the US and Canada LED USB Connection When this crossed...

Page 54: ...erson Cover Do not remove cover or dissemble case There are no adjustable components inside the instrument If a malfunction is found refer to an authorized service person Manual Do not attempt to serv...

Page 55: ...c Technical support contact information Additional product information and special offers NanoEntek Inc 851 14 Seohae ro Paltan myeon Hwaseong si Gyeonggi do 18531 Korea Tel 82 2 6220 7940 Fax 82 2 62...

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Page 57: ...40 Fax 82 2 6220 7999 NanoEntek America Inc 220 Bear Hill Road Suite 102 Waltham MA 02451 USA Tel 1 781 472 2558 Fax 1 781 790 5649 EC Representative MT Promedt Consulting GmbH Ernst Heckel Stra e 7 6...

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