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10

VII.Trouble shooting

Fault

Cause

Disposition

Electric system

No light shown in the field

of view using halogen

lamp

The power switch is not turned

on.

Turn on the power switch.

The halogen lamp is damaged. Replace the halogen lamp.

The fuse is damaged.

Replace the fuse.

The connector of the electric

chassis is in bad contact.

Check and have professional repair it.

The halogen lamp mounted is

nonconforming.

Use a conforming halogen lamp.

Optical system and imaging

There is a black shadow

on the edge of the field of

view

or

unevenly

illuminated, making it

impossible to observe the

whole field of view.

The nosepiece has not been

turned to the fixed position.

Turn the nosepiece to the fixed position.

The filament image deviates

from the center of the collector. Reposition the lighting bulb.

There is dirt or oil on the

surface of the objective,

eyepiece or condenser

Wipe the lens surface or replace the

lens.

Oil or dust is found in the

field of view.

There is oil or dust on the

eyepiece lens.

Wipe the eyepiece.

Defocusing

or

low

resolution

The objective is damaged.

Repair the objective (by a professional).

There is oil or dust on the

surface of the lens of the

objective or eyepiece.

Wipe the objective or the eyepiece.

The aperture of the aperture

diaphragm is too small.

Adjust the aperture of the aperture

diaphragm based on the objective

magnification (or numerical aperture)

used.

The objective deviates from

the light path.

Turn the nosepiece to the fixed position.

The focal plane of the

image is inclined (brighter

on one side and darker on

the other)

The lighting bulb is seriously

inclined.

Reposition the lighting bulb.

The specimen is not laid flatly. Lay the specimen flatly on the object

stage and hold it stably.

Mechanical system

The image cannot remain

clear during observation.

The focusing mechanism flows

(slides down) automatically.

Adjust the coarse adjusting hand wheel.

The fine focusing mechanism

fails

Check and have professional repair it.

The stage loosens or is

inclined.

Check and have professional repair it.

Summary of Contents for MF31 Series

Page 1: ...MF31 Series Fluorescence Microscope User Manual Guangzhou Micro shot Technology Co Ltd Tel 86 20 38262481Fax 86 20 38262491 www m shot com sales m shot com ...

Page 2: ...ntain it properly please read this manual carefully before operating this unit Caution This manual uses the following symbols for safety reminders Be sure to observe these warnings in order to operate this unit properly and safely Caution Negligence in heeding the caution of this symbol may affect the viewing performance of this unit Provide instructions and skills in operating this unit Reminder ...

Page 3: ...to this unit please stop operating the unit and contact the supplier 6 Heat of light source The lighting bulb generates high temperatures during operation Do not touch the collector lens or lamp box when the lamp is illuminated and do not touch the bulb within 10 minutes after the lamp goes out due to high temperatures arising from operation When replacing the bulb make sure it has cooled down pro...

Page 4: ...s intact If its contact is damaged the bulb may be disabled or short circuited When replacing the bulb the feet should be inserted into the holder as deeply as possible If the feet are not tightly inserted the bulb may go out or short circuit 10 Instrument handling This precision optical instrument is heavy and should be handled with care Strong impact and rough handling are strictly prohibited it...

Page 5: ... Characteristics and applications of this unit 1 II Structural features of this unit 1 2 III Installation of this unit 2 3 IV Technical specifications 4 V Components Operation 4 8 VI Using Rmind 9 VIII Troubleshooting 10 ...

Page 6: ...e Transmission lighting LED lamp is easy to use and exchange The microscope is widely used in the field of the biology medicine industry agriculture immunofluorescence at the same time it is the perfect research instrument for the department of hospital teaching and academy II Structural features of this unit 1 C mountadapter seat 2 Observation head lever 3 Eyepice 4 Epi illumination fix screw 5 D...

Page 7: ...2 Figure 02 1 Switch lever 2 Brightness knob 3 Power III Installation of this unit Connect observation head 1 Loose screw on the top of host Step 1 Step 2 Step 3 1 2 3 ...

Page 8: ...sert in eyepiece Installation of eyepiece and condenser Objective installation clockwise rotate in objective from low times to high times until objective is tightly fixed Note Condenser under stage has been adjusted well there is no need to install by users again While need to check its centering in case of shifting during shipment ...

Page 9: ...oil Optional Non cover slip 20X 40X Condenser Abbe Condenser NA 1 25 can be moved freely up and down Stage Double Layer Mechanical Stage size 210 X 140mm Moving Range 76 X 50 mm Epi fluorescence illumination 3W LED 12V 2A Blue LED EF460 490nm Optional Royal blue LED EF420 480nm Optional Green LED EF510 550nm Optional UV LED 330 380nm Transmitted light LED light source 3W LED Power Supply AC Voltag...

Page 10: ...ention Before start power please make sure voltage if meet the instrument requirement please do not use microscope if the voltage is different it may cause damage or fire 2 Sample setting Open slider holder put on slider with upper to upside Take notice do not damage slider The slider should be even put on the stage rotate stage moving wheel with X and Y to make slider into light path as figure 04...

Page 11: ...hange size of aperture to change slider contrast In common objective times smaller aperture is smaller 4 1 3 Binocular pupil distance adjustment and diopter Eyepiece tube diopter Right eyepiece tube Eyepiece e Eyepiece tube Left eyepiece Figure 06 Attention Aperture adjustment needs to match with objective field of numerical aperture FN FN is smaller aperture size should be smaller otherwise the s...

Page 12: ...ight path B Turn 40 X objective to light path The right eyepiece is used to observe the specimen in the right eyesight Adjust the coarse and find focusing hand wheel to make the specimen clearly visible C Observing the specimen on the left eyepiece If the imaging is not clear it is necessary to adjust the eyepiece s visibility so that a clear specimen image can be observed in left eye adjust diopt...

Page 13: ...4 5 3 Adjust coarse focusing knob to slowly lift stage until the oil on slide touch bottom face of objective 4 5 4 Waggle nosepiece to eliminate bubbles in oil medium then fix oil objective to make oil full of objective and slide 4 6 Using epi fluorescence illumination system 4 6 1 Adjust condenser to the lowest position diagram to the minimum size in order to reduce interrupt lights 4 6 2 If use ...

Page 14: ...e lens Please use magnifying glass to check if there is not reference substance or under dark and confused surroundings 7 If need to take off microscope lens body please notice do not touch lens outside face to avoid impression On it will reduce image definition 8 This instrument belongs to I type normal standard electrical equipment safety 9 This instrument does not belongs to AP type or APG type...

Page 15: ...il or dust is found in the field of view There is oil or dust on the eyepiece lens Wipe the eyepiece Defocusing or low resolution The objective is damaged Repair the objective by a professional There is oil or dust on the surface of the lens of the objective or eyepiece Wipe the objective or the eyepiece The aperture of the aperture diaphragm is too small Adjust the aperture of the aperture diaphr...

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