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4.5 Oil immersion
4.5.1 Using 100X objective, you need to add oil medium. Move stage to lowest position by coarse focusing knob,
drop oil on the observation area of specimen.
4.5.2 Rotate oil immersion objective(100X) to working position (the objective is marked with oil).
4.5.3 Adjust coarse focusing knob to slowly lift stage until the oil on slide touch bottom face of objective.
4.5.4 Waggle nosepiece to eliminate bubbles in oil medium, then fix oil objective to make oil full of objective and slide.
4.6 Using epi-fluorescence illumination system
4.6.1. Adjust condenser to the lowest position, diagram to the minimum size, in order to reduce interrupt lights.
4.6.2. If use bright field, pull lever of fluorescence illuminator to blank position.
