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Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer

5024111 D

21

4. Connect one end of the 2 meter USB cable (S) supplied with the cytometer to the USB 3.0

port in the PCI card on the rear of the computer, and then connect the other end of the
USB cable to the USB port labeled

COMPUTER USB 1

(T) on the rear of the cytometer.

Note:

You must use the 2 meter USB cables supplied with the cytometer to

connect the instrument to the computer.

5. Connect one end of the 2 meter USB cable (U) supplied with the cytometer to the USB 3.0

port in the PCI card on the rear of the computer, and then connect the other end of the
USB cable to the USB port labeled

COMPUTER USB 2

(V) on the rear of the cytometer.

Note:

The USB port labeled

COMPUTER USB 2

is the connection for the camera. It

must be connected to a USB 3.0 port on the rear of the computer that is labeled
for use with the SpectraMax MiniMax 300 Imaging Cytometer.

6. For the SpectraMax i3, connect the power supply to the cytometer port on the rear of

the cytometer labeled

Alternative Power Input

and connect the other end to a wall

outlet. The alternative power supply is not required for the SpectraMax i3x.

7. Set the power switch on the rear of the instrument to the On position and wait for the

instrument to complete its initialization routine.
You might be prompted to install USB drivers, which must be done before you start the
software.

CAUTION!

The instrument can be damaged if the transport locks are not

removed before you power on the instrument. See

Removing Transport Locks on

page 15

.

Calibrating the Cytometer

When you first install the SpectraMax MiniMax 300 Imaging Cytometer, the well image needs
to be calibrated. This procedure can be done only by trained personnel.

Transmitted Light (TL) Detection Cartridge

The Transmitted Light (TL) Detection Cartridge provides white LED illumination to do
brightfield, transmitted-light imaging when you use the SpectraMax MiniMax 300 Imaging
Cytometer. The StainFree™ Cell Detection Algorithm eliminates cell staining for cell counting
and confluency measurements using proprietary transmitted light analysis technology.

The Transmitted Light (TL) Detection Cartridge occupies one slot in the detection cartridge
drawer.

Summary of Contents for SpectraMax MiniMax 300

Page 1: ...5024111 D October 2018 SpectraMax MiniMax 300 Imaging Cytometer Installation Guide...

Page 2: ...t and or license to use or permit others to use such manufacturers and or their product names as trademarks Each product is shipped with documentation stating specifications and other technical inform...

Page 3: ...r Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate...

Page 4: ...s in the PCI card on the rear of the computer to connect the computer to the instrument Install the software before you connect the instrument to the computer but do not start the software until after...

Page 5: ...ter to Never 6 Set Sleep Sleep After to Never 7 Set Sleep Hibernate After to Never 8 Set USB Settings USB Selective Suspend Setting to Disabled 9 Click OK 10 Return to the Control Panel Home page 11 C...

Page 6: ...latform Transmitted Light TL Detection Cartridge For a complete list of the contents of the package see the enclosed packing list The SpectraMax MiniMax 300 Imaging Cytometer is operated by a computer...

Page 7: ...instrument To unpack the cytometer 1 Inspect the box for damage that occurred during transportation Inform the supplier immediately and keep the damaged packaging 2 With the box facing up as indicated...

Page 8: ...nts in the optics tower Optics Protection for Shipment Description A 4 Wing nuts and bolts B 6 Transport bracket and retaining screws C 3 Optics plate retaining screws D 3 Optics cover and retaining s...

Page 9: ...e last screw the optics plate might pop into position 5 Gently remove pressure from the optics plate to let the springs below the plate extend 6 Store the optics plate retaining screws in the bag that...

Page 10: ...g materials including the optics access cover and foam block for future transport needs Do not use tools that can damage the packaging or the instrument CAUTION When transporting the instrument warran...

Page 11: ...reader Optics Access Cover Bottom of Microplate Reader Description F Optics access cover G 4 Screws 4 Remove the optics access cover F from the microplate reader and store the optics access cover and...

Page 12: ...e the laser interlock bracket H to the bottom of the microplate reader 8 Attach the other laser interlock bracket H with the interlock tab I inserted into the corresponding alignment slot J and then u...

Page 13: ...on L Optics tower M Optics access opening N 4 Microplate reader feet O 4 Microplate reader foot openings WARNING PINCH HAZARD There is limited clearance between the bottom of the microplate reader and...

Page 14: ...reader to start the bolt and engage the threads 13 Attach the other three bolts to secure the cytometer to the microplate reader Make sure that the bolts are properly seated on the bottom of the cyto...

Page 15: ...cks in place until after you attach the cytometer to the microplate reader See the installation guide or user guide Illustration Part Number Description YW 000 006 2 0 mm hex key Required Tool CAUTION...

Page 16: ...Detection cartridge drawer transport lock 3 Slide the detection cartridge drawer forward until it is outside of the instrument 4 Lower the detection cartridge drawer transport lock to remove it from t...

Page 17: ...ion 6 Plate drawer transport lock 8 Use the 2 0 mm hex key to loosen screw 1 in the upper left corner of the transport lock until the lock disconnects from the instrument frame The screw has a retaini...

Page 18: ...instrument and one controls the camera A third USB cable connects the microplate reader to the cytometer Required Accessories Part Number Description 5018965 USB interconnection cable 0 9 meter 3 foo...

Page 19: ...er switch 2 Off 3 On To connect the cytometer cables 1 Place the instrument and the computer on a work area with sufficient space for the two devices and the required cables To ensure sufficient venti...

Page 20: ...her end of the USB cable to the microplate reader USB port R on the rear of the microplate reader USB Connections Description P USB interconnection cable 0 9 meter 3 foot provided with the cytometer Q...

Page 21: ...eter labeled Alternative Power Input and connect the other end to a wall outlet The alternative power supply is not required for the SpectraMax i3x 7 Set the power switch on the rear of the instrument...

Page 22: ...rear slot 4 is reserved for the SpectraMax Injector Cartridge Note When you use the software in offline mode all detection cartridges display on the Settings dialog CAUTION To prevent damage to the in...

Page 23: ...for the detection cartridge slot Note Some detection cartridges occupy more than one slot Item Detection Cartridge Drawer Top View 1 Holder pin 2 Holder pin 3 Detection cartridge connector 4 Retaining...

Page 24: ...curely fasten all retaining clips to the retaining rods on both sides of the detection cartridge drawer Item Detection Cartridge Retaining Clips and Retaining Rods 1 Detection cartridge 2 Retaining cl...

Page 25: ...or you can use fluorescent excitation from the bottom of the plate See Transmitted Light TL Detection Cartridge on page 21 Item Description 1 Transmitted Light TL Detection Cartridge 2 Path of white...

Page 26: ...ty of Molecular Devices LLC or their respective owners Specifications subject to change without notice Patents www moleculardevices com productpatents FOR RESEARCH USE ONLY NOT FOR USE IN DIAGNOSTIC P...

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