Appendix A: Instrument Specifications
5016208 D
39
Item
Description
Wavelength range
Excitation: 300 nm to 630 nm
Emission: 390 nm to 650 nm
Detection limit, guaranteed
25 fmol/200 µL fluorescein (125 pM), black 96-well plate;
1 second integration time
15 fmol/75 µL fluorescein (200.3 pM), black 384-well plate;
400 ms integration time
Detection limit, optimized
8 fmol/200 μL fluorescein (40 pM), black 96-well plate;
1 second integration time
4 fmol/75 μL fluorescein (53.3 pM), black 384-well plate;
400 ms integration time
Filter slide positions
6
Supplied wavelengths
Excitation: 485 nm, 535 nm
Emission: 535 nm, 595 nm, 625 nm
Additional filters are available.
Light source
High power LEDs and deuterium lamp
Detector
Photomultiplier tube, Single Photon Counting
Integration Time per Well
10 ms to 10000 ms
Table A-3: Fluorescence Intensity Bottom Measurement Specifications (FilterMax F5)
Item
Description
Wavelength range
(FilterMax F3)
400 nm to 650 nm
Wavelength range
(FilterMax F5)
400 nm to 700 nm
Detection limit, guaranteed
(FilterMax F3)
6 fmol/200 μL ATP (30 pM), white 96-well plate
2 fmol, 50 μL ATP (40 pM) white 384-well plate
Detection limit, optimized
(FilterMax F3)
2 fmol/200 μL ATP (10 pM), white 96-well plate
0.75 fmol/50 μL ATP (15 pM), white 384-well plate
Detection limit, guaranteed
(FilterMax F5)
6 fmol/200 μL ATP (30 pM), white 96-well plate
2 fmol/50 μL ATP (40 pM), white 384-well plate
Detection limit, optimized
(FilterMax F5)
2 fmol/200 μL ATP (10 pM), white 96-well plate
0.75 fmol/50 μL ATP (15 pM), white 384-well plate
Linear dynamic range
5 decades
Detector
Photomultiplier tube, Single Photon Counting
Integration time per well
10 ms to 10000 ms
Table A-4: Luminescence Measurement Specifications
