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FilterMax F3 and FilterMax F5 Multi-Mode Microplate Readers User Guide

23

5016208 D

Loading and Unloading Filter Slides

The buttons on the instrument front pane enable you to exchange filter slides at any time
between reads. Each filter slide has an identification code built into the slide that enables the
SoftMax Pro Software to recognize the filter configuration. The software can store up to 31
excitation and 31 emission slide configurations.

CAUTION!

Handle filter slides by the tab only. Do not touch filter surfaces with fingers.

Fingerprints left on filters can cause inaccurate measurement results.

Item

Description

1

EX FILTER

- Press to eject the excitation filter slide

2

EM FILTER

- Press to eject the emission filter slide

3

Filter compartment door

4

Excitation filter slide

5

Emission filter slide

Filter Compartment

Note:

Slides labeled EXP or EMP can also house polarization filters and are designed

for use with the FilterMax F5 instrument only.

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Summary of Contents for FilterMax F3

Page 1: ...5016208 D October 2018 FilterMax F3 and FilterMax F5 Multi Mode Microplate Readers User Guide ...

Page 2: ... any right and or license to use or permit others to use such manufacturers and or their product names as trademarks Each product is shipped with documentation stating specifications and other technical information Molecular Devices products are warranted to meet the stated specifications Molecular Devices makes no other warranties or representations express or implied including but not limited to...

Page 3: ...Panel Controls and Indicators 21 Loading and Unloading Filter Slides 23 Loading and Unloading Plates 24 Chapter 4 Maintenance and Troubleshooting 27 Cleaning the Instrument 27 Preventive Maintenance 27 Replacing Fuses 28 Replacing the Deuterium Lamp in the FilterMax F5 30 Packing the Instrument 32 Troubleshooting 35 Obtaining Support 36 Appendix A Instrument Specifications 37 Measurement Specifica...

Page 4: ...sed in a manner not specified by Molecular Devices the protection provided by the equipment might be impaired Warnings Cautions Notes and Tips All warning symbols are framed within a yellow triangle An exclamation mark is used for most warnings Other symbols can warn of other types of hazards such as biohazard electrical or laser safety warnings as are described in the text of the warning Follow t...

Page 5: ...e location of the Protective Ground Terminal Indicates the location of a fuse Required in accordance with the Waste Electrical and Electronic Equipment WEEE Directive of the European Union It indicates that you must not discard this electrical or electronic product or its components in domestic household waste or in the municipal waste collection system For products under the requirement of the WE...

Page 6: ... in to 11 8 in gap between the rear of the instrument and the wall Power off the instrument when not in use Chemical and Biological Safety Normal operation of the instrument can involve the use of materials that are toxic flammable or otherwise biologically harmful When you use such materials observe the following precautions Handle infectious samples based on good laboratory procedures and method...

Page 7: ...ver try to exchange labware reagents or tools while the instrument is operating Never try to physically restrict the moving components of the instrument Keep the instrument work area clear to prevent obstruction of the movement WARNING Do not attempt to access the interior of the instrument unless specifically instructed to do so The moving parts inside the instrument can cause injury Do not opera...

Page 8: ...rements The other slide is reserved for the emission filters you use for fluorescence and some luminescence measurements Each slide can hold up to six filters You can integrate the FilterMax F3 and FilterMax F5 with the StakMax Microplate Handling System FilterMax F3 Multi Mode Microplate Reader Available read modes Visible Absorbance ABS at wavelengths between 340 nm and 650 nm Fluorescence Inten...

Page 9: ...logy Read height optimization for top read Temperature control WARNING WARNING The FilterMax F5 uses a deuterium lamp that emits UV radiation Never view light from the deuterium lamp directly without approved eye wear Protect skin from exposure to the light emitted by the lamp Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax Pro Data Acqui...

Page 10: ...ilterMax F5 can maintain the temperature inside the plate chamber at ambient plus 4 C 1 C up to 45 C You use the SoftMax Pro Software to set and control the temperature See the SoftMax Pro Software application help or user guide Note The temperature sensors detect the temperature of the air inside the chamber not the temperature of the samples in the plate If you use the instrument to warm the sam...

Page 11: ...est Version SoftMax Pro Software installation guide with product key 5016209 Instrument installation guide YW 000 005 1 5 mm hex key YW 000 006 2 0 mm hex key GU V3 244 RS 232 9 pin serial connection cable VZ 000 012 USB to RS 232 adapter cable If the computer does not have an RS 232 serial port then connect the supplied USB to RS 232 adapter cable to a USB port on the computer 4400 0002 or 4400 0...

Page 12: ...d packaging 2 Open the box lid and remove the accessories box 3 Use the molded foam packaging to lift the instrument out of the box 4 Remove the foam packaging and the plastic bag 5 Move the instrument to a dry flat area Removing Transport Locks There is a transport lock on the plate drawer to protect the instrument from damage during shipment You must remove the transport lock before you power on...

Page 13: ... mm hex key to loosen screw 1 in the upper left corner of the transport lock until the lock disconnects from the instrument frame The screw has a retaining washer to prevent removal from the lock 3 Use the 2 0 mm hex key to loosen screw 2 and screw 3 until the lock disconnects from the plate drawer The screws have retaining washers that prevent removal from the lock 4 Gently close the plate chambe...

Page 14: ...ent Illustration Part Number Description GU V3 244 RS 232 9 pin serial connection cable VZ 000 012 USB to RS 232 adapter cable If the computer does not have an RS 232 serial port then connect the supplied USB to RS 232 adapter cable to a USB port on the computer 4400 0002 or 4400 0036 Power cord 1 meter 3 3 foot Required Accessories Item Description 1 Power Switch 2 Power Port 3 USB Port ...

Page 15: ...n on the wrong type of filter slide You can install individual excitation filters in any location on an excitation slide and you can install individual emission filters on an emission slide You should leave the original standard filter slide configurations in the required format for compliance testing See Available Standard Filter Slides on page 44 Note You should not reconfigure standard filter s...

Page 16: ...ter from a slot that houses a polarization filter use tweezers to gently slide the polarization filter out far enough to allow the removal of the excitation or emission filter You should not completely remove the polarization filter from the slide Slides that house polarization filters are designed for use with the FilterMax F5 instrument only See Removing and Installing Polarization Filters on pa...

Page 17: ...velength and type of filter Both are printed on the filter The slot where you installed the filter The slot closest to the slider tab is slot 1 and the slot furthest away is slot 6 The filter slide identification number printed on the slide 7 Update or create the slide configuration in the SoftMax Pro Software See the SoftMax Pro Data Acquisition and Analysis Software User Guide or the application...

Page 18: ...r slide Item Description 1 Filter slide 2 Filter slot 1 3 Polarization filter 2 Use tweezers to handle remove or install the polarization filter To install a polarization filter use tweezers to gently slide the polarization filter into the guide slots over the excitation or emission filter in the slide until the polarization filter is securely in place To remove a polarization filter use tweezers ...

Page 19: ...e compressed air nitrogen or canned dusters that do not use freon or similar propellents to blow dust from the filter Other sources of compressed gas can leave a residue on the filter surface 3 Blow air from a canned duster or bulb type blower over the filter surface to remove dust Periodically clean the bulb blower to prevent surface contamination Alternatively gently wipe dust from the filter su...

Page 20: ...20 cm to 30 cm 7 9 in to 11 8 in gap between the rear of the instrument and the wall To turn the instrument on or off press the power switch to place the rocker in the on or off position Each time you turn the instrument on an initialization procedure moves the optics and plate drawers to their home positions For optimal performance allow the instrument to warm up for 30 minutes before you do meas...

Page 21: ...front panel has three buttons and LED status indicators that provide information about instrument status The buttons enable you to open and close the plate drawer and eject the filter slides Item Description 1 Deuterium lamp housing cover FilterMax F5 instrument only 2 Status LEDs and control buttons 3 Filter compartment 4 Plate drawer ...

Page 22: ...rgency stop To cancel an emergency stop power the instrument off and then back on LED Color and Activity Instrument Status Green LED solid glow The instrument is in the ready state Green LED blinking A problem occurred during initialization The instrument is not ready for operation Green LED solid glow and Amber LED solid glow The instrument is busy Wait until the amber LED turns off before you at...

Page 23: ...are can store up to 31 excitation and 31 emission slide configurations CAUTION Handle filter slides by the tab only Do not touch filter surfaces with fingers Fingerprints left on filters can cause inaccurate measurement results Item Description 1 EX FILTER Press to eject the excitation filter slide 2 EM FILTER Press to eject the emission filter slide 3 Filter compartment door 4 Excitation filter s...

Page 24: ... Software User Guide or the application help Note You need to modify the slide configuration in the software only if the software does not recognize the slide or if you change the filters installed on the slide To unload filter slides 1 On the instrument front panel Press EX FILTER to eject the excitation filter slide Press EM FILTER to eject the emission filter slide 2 Open the filter compartment...

Page 25: ...ulti Mode Microplate Readers User Guide 25 5016208 D Item Description 1 open and close the plate drawer 2 Well A1 on the plate for landscape orientation 3 Plate drawer Plate Drawer With Plate Loaded 3 Press to close the plate drawer ...

Page 26: ... white plates With strong signals white plates can produce crosstalk For Fluorescence Polarization FP reads use solid black plates You should use an unlidded plate for fluorescence polarization measurements The SoftMax Pro Software includes a plate list and you can use the software to define additional plate types with the manufacturer s specifications for well size spacing and distance from the p...

Page 27: ...ecular Devices for repair Molecular Devices does not accept items that have not been decontaminated where applicable to do so If parts are returned they must be enclosed in a sealed plastic bag that states that the contents are safe to handle and are not contaminated WARNING BIOHAZARD Always wear gloves when operating the instrument and during cleaning procedures that could involve contact with ei...

Page 28: ...itching it on check to see whether the supplied power cord is securely plugged into a functioning power outlet and to the power port on the rear of the instrument If the power failed while the instrument was on check that the power cord is not loose or disconnected and that power to the power outlet is functioning properly If these checks fail to remedy the loss of power replace the fuses You can ...

Page 29: ...3 Use a small flat head screwdriver to gently press on the carrier release tab and then pull the fuse carrier to remove it from the instrument Item Description 1 Fuse carrier 2 Carrier release tab Release Fuse Carrier 4 Gently pull the old fuses from the carrier by hand 5 Place new fuses into the carrier by hand 6 Press the fuse carrier into the instrument until the carrier snaps into place 7 Plug...

Page 30: ...e the deuterium lamp WARNING WARNING The FilterMax F5 uses a deuterium lamp that emits UV radiation Never view light from the deuterium lamp directly without approved eye wear Protect skin from exposure to the light emitted by the lamp WARNING BURN HAZARD Power off the instrument disconnect the power cord and wait a minimum of 30 minutes for the deuterium lamp to cool before you perform this maint...

Page 31: ...3 Lamp power cable and connector 4 Alignment notch 5 Flat head screws captive 6 Deuterium lamp 5 On the lamp power cable connector press down on the locking latch to unlatch the connector 6 With the clip unlatched disconnect the lamp power cable from the instrument 7 Use the flat head screwdriver to loosen the flat head screws that secure the deuterium lamp These captive screws are designed to rem...

Page 32: ...to tighten the two hex screws that secure it in place 13 Reconnect the power cable to the instrument 14 Power on the instrument Packing the Instrument To minimize the possibility of damage during storage or shipment the instrument should be repacked only in the original packaging materials To order replacement packing materials contact your Molecular Devices representative Correctly repacking the ...

Page 33: ...LTER 3 Filter compartment door 4 Excitation filter slide 5 Emission filter slide 3 Open the filter compartment door CAUTION Handle filter slides by the tab only Do not touch filter surfaces with fingers Fingerprints left on filters can cause inaccurate measurement results 4 Grasp the slide by the tab and pull until the slide is free of the track Store the slide in the toolbox 5 On the front panel ...

Page 34: ... transport lock with the holes on the end of the plate drawer Item Description 1 Screw 1 fastens the lock to the internal frame of the instrument 2 Screw 2 Fastens the lock to the plate drawer 3 Screw 3 Fastens the lock to the plate drawer 4 Transport lock 5 Plate drawer 6 Plate chamber door open 13 Use the 2 0 mm hex key to tighten screws 2 and 3 on the transport lock to attach the lock to the pl...

Page 35: ...ect the fuses The fuses look burned or the filaments are broken Replace the fuses See Replacing Fuses on page 28 The green LED is not illuminated the power is on the fuses are okay and the instrument does not operate Contact Molecular Devices technical support See Obtaining Support on page 36 Both the green and the amber LEDs are illuminated The instrument is busy Wait until the amber LED turns of...

Page 36: ...e upgrades safety data sheets and other resources If you still need assistance after consulting the Knowledge Base you can submit a request to Molecular Devices Technical Support Please have your instrument serial number or Work Order number and your software version number available when you call WARNING BIOHAZARD It is your responsibility to decontaminate components of the instrument before you ...

Page 37: ... 3 5 OD Resolution 0 1 mOD from 0 OD to 3 5 OD Linearity 0 75 and 0 010 OD from 0 1 OD to 3 0 OD 399 nm 0 75 and 0 010 OD from 0 1 OD to 2 5 OD 400 nm Filter slide positions 6 Supplied wavelengths FilterMax F3 405 nm 450 nm 492 nm 620 nm Additional filters are available Supplied wavelengths FilterMax F5 260 nm 340 nm 405 nm 450 nm 595 nm 620 nm Additional filters are available Measurement method T...

Page 38: ...te 1 second integration time 0 4 fmol 75 μL fluorescein 5 3 pM black 384 well plate 400 ms integration time Detection limit optimized FilterMax F5 0 3 fmol 200 μL fluorescein 1 5 pM black 96 well plate 1 second integration time 0 1 fmol 75 μL fluorescein 1 3 pM black 384 well plate 400 ms integration time Measurement range Up to 500 million RFU Linear dynamic range Up to 6 decades in a single plat...

Page 39: ...ounting Integration Time per Well 10 ms to 10000 ms Table A 3 Fluorescence Intensity Bottom Measurement Specifications FilterMax F5 Item Description Wavelength range FilterMax F3 400 nm to 650 nm Wavelength range FilterMax F5 400 nm to 700 nm Detection limit guaranteed FilterMax F3 6 fmol 200 μL ATP 30 pM white 96 well plate 2 fmol 50 μL ATP 40 pM white 384 well plate Detection limit optimized Fil...

Page 40: ...e 400 ms integration time Uniformity optimized 1 mP at 10 nM fluorescein black 96 well plate 1 second integration time 1 5 mP at 10 nM fluorescein black 384 well plate 400 ms integration time Filter slide positions 6 Supplied wavelengths Excitation 485 nm Emission 535 nm Additional filters are available Light source High power LEDs and deuterium lamp Detector Photomultiplier tube Single Photon Cou...

Page 41: ...nd integration time 15 amol 100 µL Europium 0 15 pM white 384 well plate 400 ms integration time Linear dynamic range 5 decades Filter slide positions 6 Supplied wavelengths Excitation 370 nm Emission 625 nm Note TRF measurements can be done only with the 370 nm broadband filter supplied by Molecular Devices Use of this filter is required to achieve the specified performance Light source High powe...

Page 42: ...C 4 F to 122 F transient up to 10 hours Humidity restrictions 15 to 75 non condensing at 30 C 86 F Altitude restrictions Up to 2000 m 6562 ft Air pressure restrictions 54 kPa to 106 kPa 7 8 PSI to 15 4 PSI Sound pressure level Maximum sound pressure 75 dBA Maximum sound pressure at one meter 68 dBA Installation category II Pollution degree 2 Fuses 250 V 3 amp 5 x 20 mm slow blow UL recognized CSA ...

Page 43: ...5016208 D 43 Appendix B System Diagram and Dimensions Item Description 1 Width 39 cm 15 4 in 2 Height 24 cm 9 5 in 3 Depth 58 cm 22 9 in Dimensions of the FilterMax F3 and FilterMax F5 B ...

Page 44: ...Enabled 2 485 nm FL Enabled 3 405 nm ABS Enabled 4 450 nm ABS Enabled 5 492 nm ABS Enabled 6 620 nm ABS Enabled EX3 Excitation Filter Slide Position Wavelength Read Mode Status 1 465 nm FL Enabled 2 535 nm FL Enabled 3 0 nm NA Empty 4 0 nm NA Empty 5 0 nm NA Empty 6 0 nm LUM Enabled EM 3 Emission Filter Slide FilterMax F5 Standard Filter Slides Position Wavelength Read Mode Status 1 260 nm ABS Ena...

Page 45: ...ization Capable Position Wavelength Read Mode Status 1 595 nm FL Enabled 2 535 nm FL Enabled 3 535 nm FP Enabled 4 535 nm FP Enabled 5 625 nm FL TRF Enabled 6 0 nm LUM Enabled EMP 1 Emission Filter Slide Polarization Capable FilterMax F5 Standard Filters Available for Purchase Position Wavelength Read Mode Status 1 260 nm ABS Enabled 2 280 nm ABS Enabled 3 320 nm ABS Enabled 4 0 nm NA Empty 5 0 nm...

Page 46: ... 1 0 nm NA Empty 2 0 nm NA Empty 3 0 nm NA Empty 4 0 nm NA Empty 5 900 nm PathCheck Enabled 6 998 nm PathCheck Enabled EX 7 PathCheck filter Slide I Part Number 5011295 Customizable Filter Slides The following filter slides have no filters installed and are available for you to configure custom filter slides Part Number Filter Slide 5008587 EXP 20 Excitation Filter Slide polarization capable 50085...

Page 47: ...on 3 0200 5060 SpectraTest FL1 Fluorescence Validation Plate Gemini EM Gemini XPS SpectraMax iD3 iD5 i3x i3 M2 M2e M3 M4 M5 M5e FlexStation 3 0200 6186 SpectraTest LM1 Luminescence Validation Plate SpectraMax iD3 iD5 i3x i3 M3 M4 M5 M5e SpectraMax L FlexStation 3 0200 2420 Cuvette Absorbance Validation Set SpectraMax Plus 384 ABS Plus M2 M2e M3 M4 M5 M5e 0200 7200 Multi Mode Validation Plate Filte...

Page 48: ...Equipment Classification Group 1 Class A This equipment is designated as scientific equipment for laboratory use that intentionally generate and or use conductively coupled radio frequency energy for internal functioning and are suitable for use in all establishments other than domestic and those directly connected to a low voltage power supply network which supply buildings used for domestic purp...

Page 49: ...rty of Molecular Devices LLC or their respective owners Specifications subject to change without notice Patents www moleculardevices com productpatents FOR RESEARCH USE ONLY NOT FOR USE IN DIAGNOSTIC PROCEDURES 2018 Molecular Devices LLC All rights reserved 5016208 D ...

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