Millipore Amicon Ultra-2, User Manual, Page: 9 / 11

Amicon
®
Ultra-2 Centrifugal Filter Devices
9
Chemical Compatibility
Amicon
®
Ultra centrifugal devices are intended for use with biological fluids and aqueous solutions. Before use, check the sample
for chemical compatibility with the device.
Table 6. Chemical Compatibility of Amicon
®
Ultra Filter Devices.
Acids Concentration Concentration
Acetic acid ≤ 50%* Phosphoric acid ≤ 30%
Formic acid ≤ 5%* Sulfamic acid ≤ 3%
Hydrochloric acid ≤ 1.0 M Sulfuric acid ≤ 3%
Lactic acid ≤ 50% Trichloroacetic acid (TCA) ≤ 10%*
Nitric acid ≤ 10% Trifluoroacetic acid (TFA) ≤ 30%*
Alkalis
Ammonium hydroxide ≤ 10% Sodium hydroxide ≤ 0.5 M
Alcohols
n-Butanol ≤ 70% Isopropanol ≤ 70%
Ethanol ≤ 70% Methanol ≤ 60%
Detergents
Alconox
®
detergent ≤ 1% Sodium dodecyl sulfate (SDS) ≤ 0.1%
CHAPS detergent ≤ 0.1% Tergazyme
®
detergent ≤ 1%
Lubrol
®
PX detergent ≤ 0.1% Triton
®
X-100 surfactant ≤ 0.1%
Nonidet™ P-40 surfactant ≤ 2% Tween
®
20 surfactant ≤ 0.1%
Sodium deoxycholate ≤ 5%
Organic solvents
Acetone Not recommended Ethyl acetate Not recommended
Acetonitrile ≤ 20% Formaldehyde ≤ 5%
Benzene Not recommended Pyridine Not recommended
Carbon tetrachloride Not recommended Tetrahydrofuran Not recommended
Chloroform Not recommended Toluene Not recommended
Dimethyl sulfoxide (DMSO) ≤ 5%*
Miscellaneous
Ammonium sulfate Saturated Phenol ≤ 1%
Diethyl pyrocarbonate ≤ 0.2% Phosphate buffer (pH 8.2) ≤ 1 M
Dithiothreitol (DTT) ≤ 0.1 M Polyethylene glycol ≤ 10%
Glycerine ≤ 70% Sodium carbonate ≤ 20%
Guanidine HCl ≤ 6 M Tris buffer (pH 8.2) ≤ 1 M
Imidazole ≤ 100 mM Urea ≤ 8 M
Mercaptoethanol ≤ 0.1 M
* Contact with this chemical may cause materials to leach out of the component parts. Solvent blanks are recommended
to determine whether leachables represent potential assay interferences.