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Millipore Amicon Ultra-2, User Manual, Page: 10 / 11

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Millipore Amicon Ultra-2 User Manual Download Page 10

Amicon

®

 Ultra-2 Centrifugal Filter Devices

10

Product Ordering Information

This section lists the catalogue numbers for Amicon

®

 Ultra Ultrafiltration Devices. See the Technical Assistance section for contact 

information. You can purchase these products on-line at 

www.sigmaaldrich.com/products

.

MWCO

Qty/ 

pk

Amicon

®

 

Ultra-0.5 

device

Amicon

®

 

Ultra-2 

device

Amicon

®

 

Ultra-4 

device

Amicon

®

 

Ultra-15 

device

3K

8 

24 

96

 

500

UFC500308

 

UFC500324

 

UFC500396

 

UFC5003BK

 

UFC200324

 

UFC800308

 

UFC800324

 

UFC800396

 

UFC900308

 

UFC900324

 

UFC900396

10K

8 

24 

96

 

500

UFC501008

 

UFC501024

 

UFC501096

 

UFC5010BK

 

UFC201024

UFC801008

 

UFC801024

 

UFC801096

UFC901008

 

UFC901024

 

UFC901096

10K 

IVD*

8 

24 

96

UFC801008D

 

UFC801024D

 

UFC801096D

UFC901008D

 

UFC901024D

 

UFC901096D

30K

8 

24 

96

 

500

UFC503008

 

UFC503024

 

UFC503096

 

UFC5030BK

 

UFC203024

UFC803008

 

UFC803024

 

UFC803096

 

UFC903008

 

UFC903024

 

UFC903096

50K

8 

24 

96

 

500

UFC505008

 

UFC505024

 

UFC505096

 

UFC5050BK

 

UFC205024

UFC805008

 

UFC805024

 

UFC805096

 

UFC905008

 

UFC905024

 

UFC905096

 

100K

8 

24 

96

 

500

UFC510008

 

UFC510024

 

UFC510096

 

UFC5100BK

 

 

UFC210024

UFC810008

 

UFC810024

 

UFC810096

 

UFC910008

 

UFC910024

 

UFC910096

 

*    Amicon

® 

Ultra-4 and -15 10K devices are for in vitro diagnostic (IVD) use. All other devices are for research use only.

Summary of Contents for Amicon Ultra-2

Page 1: ...pun in a swinging bucket or fixed angle rotor Amicon Ultra 2 devices are supplied non sterile and are for single use only The Amicon Ultra 2 product line includes 5 different cutoffs Molecular Weight...

Page 2: ...Failure to fully seat the device in the filtrate collection tube and push the concentrate collection tube firmly onto the device may result in the device breaking during centrifugation See figure bel...

Page 3: ...mL protein in 100 mM NaCl 50 L of 40 mg mL protein in 100 mM NaCl 100 mM NaCl 50 L of 40 mg mL protein in 12 3 mM NaCl 12 3 mM NaCl Add 1 95 mL of 10 mM NaCl or exchange buffer 2 mL of 1 mg mL protei...

Page 4: ...10 to 20 minutes of centrifugation the lowest concentrate volume 30 70 L is reached after spinning for 10 to 60 minutes Figure 1 Typical Filtrate Volume vs Spin Time for Amicon Ultra 2 Device Swingin...

Page 5: ...nd 10K BSA for 30K and 50K and IgG for 100K n 8 Shaded volumes were used for the calculation of protein recovery in Table 5 Table 3 Typical Concentrate Volume Concentration Factor v Spin Time Fixed An...

Page 6: ...nging bucket rotor 4 000 g or 35 fixed angle rotor 7 500 g 2 mL starting volume room temperature n 12 Factors that determine sample recovery include the nature of the protein solute relative to the de...

Page 7: ...te collection tube and concentrate collection tube before use 2 Fill filter device with solution and reweigh 3 Assemble device in filtrate collection tube and centrifuge per instructions 4 Collect the...

Page 8: ...rum or plasma do not exceed 5 400 x g Active membrane area 1 cm2 Hold up volume 5 L Dimensions Filter device and tube Length concentration mode device in filtrate tube 119 7 mm 4 71 in Length recovery...

Page 9: ...ent 0 1 Tergazyme detergent 1 Lubrol PX detergent 0 1 Triton X 100 surfactant 0 1 Nonidet P 40 surfactant 2 Tween 20 surfactant 0 1 Sodium deoxycholate 5 Organic solvents Acetone Not recommended Ethyl...

Page 10: ...FC900396 10K 8 24 96 500 UFC501008 UFC501024 UFC501096 UFC5010BK UFC201024 UFC801008 UFC801024 UFC801096 UFC901008 UFC901024 UFC901096 10K IVD 8 24 96 UFC801008D UFC801024D UFC801096D UFC901008D UFC90...

Page 11: ...office nearest you go to www sigmaaldrich com offices Technical Assistance Visit the tech service page on our web site at www sigmaaldrich com techservice Standard Warranty The applicable warranty fo...

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