Amicon
®
Ultra-2 Centrifugal Filter Devices
5
Table 2. Typical Concentrate Volume / Concentration Factor vs. Spin Time, Swinging Bucket Rotor
3K device
10K device
30K device
50K device
100K device
Spin Time
(min)
Conc.
Volume
(μL)
Conc.
Factor
(x)
Conc.
Volume
(μL)
Conc.
Factor
(x)
Conc.
Volume
(μL)
Conc.
Factor
(x)
Conc.
Volume
(μL)
Conc.
Factor
(x)
Conc.
Volume
(μL)
Conc.
Factor
(x)
5
281
7
91
22
1070
2
10
880
2
190
11
71
27
47
42
523
4
15
96
21
52
38
44
47
167
12
20
317
7
65
31
43
46
38
52
65
31
30
147
30
48
42
39
51
38
53
37
53
40
102
20
44
45
60
55
32
Spin conditions: Swinging bucket rotor, 4,000 × g, room temperature, 2 mL starting volume.
Protein markers used: Cytochrome c for 3K and 10K, BSA for 30K and 50K, and IgG for 100K, n=8.
Shaded volumes were used for the calculation of protein recovery in Table 5.
Table 3. Typical Concentrate Volume/Concentration Factor v. Spin Time, Fixed Angle Rotor
3K device
10K device
30K device
50K device
100K device
Spin
Time
(min)
Conc.
Volume
(μL)
Conc.
Factor
(x)
Conc.
Volume
(μL)
Conc.
Factor
(x)
Conc.
Volume
(μL)
Conc.
Factor
(x)
Conc.
Volume
(μL)
Conc.
Factor
(x)
Conc.
Volume
(μL)
Conc.
Factor
(x)
5
137
15
80
25
879
2
10
731
3
101
21
51
39
30
71
203
10
15
60
33
37
57
22
90
61
34
20
215
10
39
51
24
85
21
99
32
63
30
106
19
25
80
20
101
18
89
17
115
40
70
29
23
87
60
45
45
Spin conditions: 35° fixed angle rotor, 7,500 × g, room temperature, 2 mL starting volume.
Protein markers used: Cytochrome c for 3K and 10K, BSA for 30K and 50K, and IgG for 100K, n=8.
Shaded volumes were used for the calculation of protein recovery in Table 5.