3.3 Fitting the accessories
Metrohm 838 Advanced Sample Processor
,
Installation
33
Conditioning the dialysis system
Before the first analysis, the dialysis cell with the fitted dialysis mem-
brane and all tubing connections must be rinsed with ultra-pure water.
Please proceed as follows:
1 Settings on the 820 IC Separation Centre
In the
«
IC Net
»
software, switch injection valve A in the 820 IC
Separation Centre to position "FILL".
2 Commissioning the peristaltic pumps
Immerse the two aspiratory tubings
68
and
72
(see
Figure 24
)
in the acceptor solution (degassed ultra-pure water).
Switch on the 838 Advanced Sample Processor and the 833
Pump Unit with their power switches.
Switch on the peristaltic pumps of both instruments in «IC
Net» in the corresponding 'Manual Control' dialog box. Please
refer to the
«
IC Net
»
software Online Help documentation for
details of operation.
Set the contact pressure on both tubing cartridges: Push the
pressure lever
49
(see
Chapter 1.3.5
) up until the solutions
are just being aspirated. Then push the pressure lever 1 fur-
ther detent position upwards to achieve an optimum contact
pressure.
Flush the dialysis system for approx. 10 minutes with ultra-
pure water and check whether solution is emerging uniformly
on the two inlet capillaries leading into the waste container.
3 Conditioning the dialysis membrane
Flush the dialysis system for approx. 20 minutes with ultra-
pure water. Check whether solution is emerging uniformly on
the two inlet capillaries leading into the waste container.
Check all tubings from the supply vessels through the tubing
cartridges and the dialysis cell through to the waste vessels
for the presence of emerging fluid. If fluid is escaping at any
point, the corresponding connection must be tightened or ex-
changed.
If air bubbles remain in the dialysis cell, unscrew the PEEK
capillary
76
(acceptor solution) and PTFE capillary
73
(sam-
ple) from the outlets
44 resp
.
47
of the dialysis cell and wait
until the air bubbles have disappeared. Then screw the tub-
ings back onto the dialysis cell.
