3.5 Connection to Metrohm IC systems
50
Metrohm 838 Advanced Sample Processor
,
Installation
Dialysis.smt
Entry of the program for the 838 Advanced Sample Processor
Dialysis program:
001 Pump
on
002 Ctrl FILL A 1
003 Ctrl Pump 833 on
004 Wait
30
s
005 Ctrl INJECT A 1
006 Ctrl Pump 833 off
007 Pump
off
008 Wait
600
009 Move
1
010 Lift
work
011 Pump
on
012 Ctrl Pump 833 on
013 Wait
120
014 Ctrl Pump 833 off
015 Wait
480
s
016 Ctrl FILL B/STEP 1
017 Wait
120
018 Ctrl
ZERO
1
019 Pump
off
−
Pump sample solution
−
Switch over injection valve in 820 to "Fill"
−
Pump acceptor solution
−
Transfer time (must be determined)
−
Switch over injection valve in 820 to "Inject"
−
Stop pumping acceptor solution
−
Stop pumping sample solution
−
Waiting time for synchronisation
−
Approach next sample
−
Move lift with needle to working height
−
Pump sample solution
−
Pump acceptor solution
−
Rinsing time
−
Stop pumping acceptor solution, start of dialysis
−
Dialysis time (120 s + 480 s = 10 min.)
−
Trigger step of suppressor unit
−
Wait until baseline is stable
−
Trigger autozero on 819/1 IC Detector
−
Stop pumping sample solution
Start-Dialysis.smt
•
Remove the data recorder by clicking on the recorder icon with
the right mouse button an selecting '
unlink
'.
Start program:
001 Move
sample
002 Lift
work
003 Ctrl Pump 833 on
004 Pump
on
005 Wait
120
s
006 Ctrl Pump 833 off
007 Wait
480
s
008 Ctrl FILL B/STEP 1
009 Wait
180
s
010 Ctrl
ZERO
1
011 Pump
off
−
Move needle to sample position
−
Move lift with needle to working height
−
Pump acceptor solution
−
Pump sample solution
−
Rinsing time
−
Stop pumping acceptor solution, start of dialysis
−
Dialysis time (780 s+ 120 s = 10 min)
−
Trigger step of suppressor unit
−
Wait until baseline is stable
−
Trigger autozero on 819/1 IC Detector
−
Switch over injection valve the 820 to "Inject"