© MEDsan
®
Biotech GmbH | Brandshofer Deich 10 | 20539 Hamburg | Germany
6
| ULTRA SBMS
24
Professional use only
V2.2 EN 2021-12-16
V2.2 EN 2021-12-16
Quick Operation Guide |
Positive Control
8
PC
FAM/SYBR
20.715
-
positive (+)
Valid PC
FAM
and
HEX
and
CY5 have value
<39
and
sigmoidal
curves
.
VIC/HEX
30.649
-
positive (+)
CY5
10.649
-
positive (+)
Invalid
PC
The positive control has failed if
one of the three channels is negative
and/or the
curves are not sigmoidal
and/or the values are
≥39
.
Attention:
However, if a
sample in the run (FAM) is positive
despite a failed PC,
the run must still be scored and the sample result is than
positive
.
H
Sample Result Interpretation
Negative Results
21
sample
FAM/SYBR
-
-
negative(-)
Negative
(NA)
HEX value
<39
and
sigmoidal curve
, FAM
negative
,
→
no virus was amplified.
VIC/HEX
26.317
-
po)
CY5
-
-
po)
Positive Results
21
sample
FAM/SYBR
15.113
-
po)
Positive
Ct 15.113
FAM
and
HEX
and
CY5 show value
<39
and
sigmoidal
curve
structure.
VIC/HEX
21.175
-
po)
CY5
32.175
-
po)
21
sample
FAM/SYBR
15.113
-
po)
Positive
Ct 15.113
No HEX value
but
FAM
and
CY5 are
positive
and show
sigmoidal curve
, → virus was amplified!
VIC/HEX
-
-
negative(-)
CY5
17.183
-
po)
Invalid Results
21
sample
FAM/SYBR
-
-
negative(-)
Invalid
No Hex value, it is not granted there is enough human
sample in the tube.
VIC/HEX
-
-
negative(-)
CY5
-
-
negative(-)
Sigmoidal curve
Sigmoidal curve
Non
