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www.bioneer.com
BQ-042-101-05
Revision : 2 (2016-07-04)
XIII.
Troubleshooting guide
Comments and suggestions
Low yield of DNA
Buffers or other reagents may have been exposed to external changes or
conditions that reduce its effectiveness. Please make sure that reagents were
stored at room temperature at all times upon arrival and all reagent bottles
were closed tightly, in order to preserve pH and stability, and to avoid
contamination.
Please check and add ethanol to the Buffer ③ (1
st
Washing) and ④ (2
nd
Washing). After adding ethanol, mix Washing Buffer well and always mark the
Washing Buffer bottles. If not, Washing Buffer remain concentrated and may
wash away the adsorbed DNA.
The lysis may have been incomplete especially in case of tissue. Please,
ensure that the sample changes clarity from turbid to clear, indicating that
protein digestion has occurred. Extend the incubation time if unlysed tissue is
still present. The amount of time for complete lysis varies depending on the
type of tissue or sample type used. If a gelatinous mass still remains after the
overnight incubation, centrifuge the sample and take the supernatant only for
extraction of DNA. A shaking water bath should be used for efficient lysis.
There is too much starting material to extract DNA. Appropriate amount of
starting material (see “product specification” in page 4) should be used for
efficient extraction of genomic DNA.
Low A
260/280
ratio
Beads may have been dried insufficiently. You must always completely dry
beads in a drying step. Remained ethanol can decrease the purity of DNA.
Take enough time to completely dry the beads.
Incomplete suspension of beads during the washing step causes salts to
remain in the purified DNA. Make the beads suspended thoroughly during the
washing process.
