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BQ-042-101-05
Revision : 2 (2016-07-04)
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Detachment
Push up the magnet plate gently.
10.
Attach the magnet plate to
MagListo
TM
rack and invert the tube 3 ~ 4 times gently until the beads
tightly bind to the magnet.
11.
Without removing the tube from
MagListo
TM
rack, pour the supernatant out and remove the remaining
supernatant using a paper towel by blotting.
12.
Repeat the above step 9 ~ 11 by adding 500 μl (mini)/ 3 ml (midi)/ 6 ml (maxi) of Buffer ③ (1
st
Washing) for additional washing to remove pigment and other debris.
13.
(2
nd
washing)
Repeat the above step 9 ~ 11 by adding 700 μl (mini)/ 5 ml (midi)/ 10 ml (maxi) of
Buffer ④ (2
nd
Washing) for additional washing.
14.
(3
rd
washing)
Repeat the above step 9 ~ 11 by adding 700 μl (mini)/ 5 ml (midi)/ 10 ml (maxi) of
absolute ethanol for additional washing.
15.
(Drying)
Completely dry the beads with the tube open and use a heat gun or a blow dryer for 1 min
(mini)/ 3 min (midi)/ 5 min (maxi) 3 cm away from the top of the tube.
(Note) Without using a heat gun or a blow dryer, place the rack lying down in a dry oven at 60℃ for 10
min (mini)/ 20 min (midi)/ 30 min (maxi).
16.
(Elution: 16-20)
Add 100 μl (mini)/ 500 μl (midi)/ 1 ml (maxi) of Buffer ⑤ (Elution) or distilled water to
the tube with the magnet plate detached and resuspend completely by pipetting or vortex mixer for 15
sec.
17.
Incubate the tube at 60℃ for 1 min.
