MagListo 5M, User Manual

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BQ-042-101-05
Revision : 2 (2016-07-04)
Summary of Reagents Volume in Each Step of DNA Extraction from Cultured Cell
Step Buffer Micro Mini Midi Maxi Page
Cultured Cell ~ 1 x 10
4
~ 1 x 10
6
~ 5 x 10
6
~1 x 10
7
P. 10
Lysis Buffer ② (Binding) 100 μl 200 μl 1 ml 1 ml P. 10
DNA Precipitation Absolute Ethanol 200 μl 400 μl 2 ml 2 ml P. 10
Bead Binding
Magnetic Nano Bead -
DNA
100 μl 100 μl 500 μl 1 ml P. 10
1
st
Washing Buffer ③ (1
st
Washing) 700 μl 700 μl 5 ml 10 ml P. 11
2
nd
Washing Buffer ④ (2
nd
Washing) 700 μl 700 μl 5 ml 10 ml P. 12
3
rd
Washing Absolute Ethanol 700 μl 700 μl 5 ml 10 ml P. 12
Elution Buffer ⑤ (Elution) 50 μl 100 μl 500 μl 1 ml P. 12