11
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BQ-042-101-05
Revision : 2 (2016-07-04)
9.
Place the tube in
MagListo
TM
-2 (micro, mini)/ MagListo
TM
-15 (midi, maxi) Magnetic Separation Rack
with the magnet plate attached and invert the tube 3~4 times gently until the beads tightly bind to the
magnet.
-
Attachment
Combine the magnet plate to the stand.
10.
Without removing the tube from
MagListo
TM
rack, carefully pour the supernatant out and completely
remove the remaining supernatant using a paper towel by blotting.
-
Discard solution
Discard solution by inverting the
MagListo™ rack. The silicone immobilizer inside the stand holds the
tubes from falling in an upside down position. When discard solution, invert the rack completely for the
solution not to smear on the rack.
11.
(1
st
washing: 11-13)
Detach the magnet plate from
MagListo
TM
rack. Add 700 μl (micro, mini)/ 5 ml
(midi)/ 10 ml (maxi) of Buffer ③ (1
st
Washing) to the tube. Close the cap and mix by vortex mixer until
the beads are fully resuspended.
-
Detachment
Push up the magnet plate gently.
12.
Attach the magnet plate to
MagListo
TM
stand and invert the tube 3~4 times gently until the beads
