LuminUltra GeneCount Q-16, Manual

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LuminUltra GeneCount ® Q-8 Q-16 qPCR
Quick Guide For Windows® - based Software
2
C O N T E N T S
Introduction ................................................................................................................ 3
Safety ....................................................................................................................... 3
Grounding Considerations............................................................................................................ 3
Risk of Electric Shock ................................................................................................................... 4
Power Supply Considerations ........................................................................................................ 4
Power cord considerations ............................................................................................................ 4
Plugging and unplugging your device ............................................................................................ 4
Placement of instrument ................................................................................................................ 4
Notes during operation ................................................................................................................ 4
Warning Signs ............................................................................................................................ 5
Maintenance of Instrument ........................................................................................... 5
Information for the User ................................................................................................................ 6
Overview .................................................................................................................. 6
Parts required ............................................................................................................................. 7
Key parameters ........................................................................................................................... 8
Set up the instrument .................................................................................................................... 9
Step 1 ............................................................................................................................... 9
Step 2 ............................................................................................................................... 9
Step 3 ............................................................................................................................. 10
Step by step software guide ....................................................................................... 10
Install the software ..................................................................................................................... 10
Launching the software .............................................................................................................. 12
The home button ............................................................................................................... 12
Resizing the window .......................................................................................................... 12
The setup page – name setup ...................................................................................................... 13
Samples and fluorescence channels set up ............................................................................ 13
Open existing experiment for template file ............................................................................ 13
The setup page – cycler setup ..................................................................................................... 14
Melting curve analysis period ............................................................................................. 14
Hot lid temperature and reaction volume .............................................................................. 15
Saving template ................................................................................................................ 15
Auto Integration Time ......................................................................................................... 15
Running the experiment and monitor status ................................................................... 15
Running amplification program ........................................................................................... 15
Running melt analysis ........................................................................................................ 16
Analysis .................................................................................................................. 16
Amplification analysis ................................................................................................................ 16
Ct Threshold ..................................................................................................................... 16
Ct Low limit ...................................................................................................................... 16
Normalize ........................................................................................................................ 16
Positive vs negative results ......................................................................................... 18
False positive results .......................................................................................................... 18
Standard curve analysis ............................................................................................. 19
Steps to perform standard curve analysis: ............................................................................. 19
Save experiment & print report ................................................................................... 20