LuminUltra GeneCount
®
Q-8 Q-16 qPCR
Quick Guide For Windows® - based Software
16
Running melt analysis
When running Melt analysis, the “Run-Melt
curve” will show the fluorescence change rate
as function of temperature.
Figure 4. Melt analysis
Analysis
There are two analysis pages: Amplification analysis and Standard curve analysis. The latter is
used for determining the quantity of the DNA targets using standard curve analysis method.
Amplification analysis
This will show the amplification curves for all
samples. We can select the samples and
study, set several parameters to this analysis.
Ct Threshold
The threshold for determining Ct is expressed
as the percentage of the saturating level of the
amplification levels after normalization. The
default Ct threshold is 10%. The bigger this
percentage, the more delayed the Ct values
are.
Ct Low limit
The minimum value of Ct is set through this parameter. This setting is helpful in determining the
base fluorescence value for amplification curve analysis. In most qPCR experiments, the
concentration of the analytes prior to amplification is low, such that the Ct value cannot be less
than 13 or so.
Normalize
When displaying amplification curve, we usually normalize the fluorescence values. This will allow
us to visualize Ct threshold value. This choice will not affect the result of the analysis.
Figure 5. Amplification analysis
