LuminUltra GeneCount Q-16 Manual Download Page 7 | Manualshive

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LuminUltra GeneCount

®

Q-8 Q-16 qPCR

Quick Guide For Windows® - based Software

7

Overview

This is a user’s guide for using LuminUltra GeneCount qPCR with Windows®-based software.

Parts required

•

GeneCount qPCR instrument unit

•

AC adapter and power cord

•

USB cable

•

A Windows®

1

- based PC

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Summary of Contents for GeneCount Q-16

Page 1: ...LuminUltra GeneCount Q 8 Q 16 qPCR Non Touch Screen Version For Windows based Software Version 0 16 for Beta release...

Page 2: ...10 Install the software 10 Launching the software 12 The home button 12 Resizing the window 12 The setup page name setup 13 Samples and fluorescence channels set up 13 Open existing experiment for tem...

Page 3: ...based on user feedback Newest versions and information are available for download from the website my luminultra com and from sales LuminUltra com Note Very important information is contained within...

Page 4: ...rument If the power cable is damaged it should be replaced with a new one of the same specifications The power supply wire should be treated with care do not place objects on the wire and do not place...

Page 5: ...tes a strange odour The instrument is dropped or its casing is damaged There is an obvious change in the function of the instrument Warning When operating or performing work on the device all relevant...

Page 6: ...re is no guarantee that interference will not occur in a particular installation If this equipment does cause harmful interference to radio or television reception which can be determined by turning t...

Page 7: ...ick Guide For Windows based Software 7 Overview This is a user s guide for using LuminUltra GeneCount qPCR with Windows based software Parts required GeneCount qPCR instrument unit AC adapter and powe...

Page 8: ...USB 2 0 Wi Fi Excitation source High endurance LED Detector Ultra low light CMOS Bio imaging chip Thermal system Solid state Peltier based Tube plate formats 0 2mL 4 or 8 tube strip Reaction volume 1...

Page 9: ...ix at end of the document about how to download decompress and install software from LuminUltra s web site Connect instrument with Windows based PC via included USB cable Power up the instrument using...

Page 10: ...pport print report function 2 We use the USB HID device interface for GeneCount This is the same USB HID for keyboard and mouse Step by step software guide Install the software The Beta version of the...

Page 11: ...LuminUltra GeneCount Q 8 Q 16 qPCR Quick Guide For Windows based Software 11...

Page 12: ...eral notable features are marked in Figure 1 Marker 1 is an indicator of whether the GeneCount USB device is found If found a message HID Device Connected is shown along with a green dot We call it HI...

Page 13: ...ed instrument can support Each reaction well can be associated with a sample In this page the user can enter a name for each sample It a sample well name field is left empty white spaces it is conside...

Page 14: ...d are configurable A cycler program can have several periods and each period can have one or more steps For example we could have pre denature period cycle period and hold period In the cycle period w...

Page 15: ...nal to noise ratio signal quality We recommend leaving this check box checked Running the experiment and monitor status In the setup page cycler setup when we are satisfied with all the settings and l...

Page 16: ...s Ct Threshold The threshold for determining Ct is expressed as the percentage of the saturating level of the amplification levels after normalization The default Ct threshold is 10 The bigger this pe...

Page 17: ...LuminUltra GeneCount Q 8 Q 16 qPCR Quick Guide For Windows based Software 17...

Page 18: ...dditional parameters that justifies the positive negative test These parameters include the saturation fluorescence level amplification efficiency and fluorescence reading signal to noise ratio False...

Page 19: ...ve to first the select the specific channel and perform such analysis Figure 7 Standard curve analysis page Steps to perform standard curve analysis Step 1 Select the fluorescence channel for such ana...

Page 20: ...riment file will contain all the settings and experiment results Two report formats will be used for report generation The excel file report is hand editable The PDF report is easier to store and use...

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