Luminex Luminex 200 System User Manual Download Page 53 | Manualshive

Page: 53 / 64

background image

x

MAP Technology

Troubleshooting

43

Figure 10 Normal Bead Detail

The histogram for the bead detail above looks like this:

Figure 11 Normal Histogram

Symptom

Possible problem

Solution

xMAP microspheres classify
too high

.

You may be using
photobleached calibration
microspheres.

Replace the calibration
microspheres with a fresh batch.
To avoid photobleaching,
protect your microspheres from
light.

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Summary of Contents for Luminex 200

Page 1: ...Luminex 200 System User Manual Lum nex...

Page 2: ...Although prepared to ensure accuracy Luminex assumes no liability for errors or omissions or for any damages resulting from the application or use of this information This guide may be updated period...

Page 3: ...S OF BUYER OR OTHER USE OR ANY LIABILITY OF BUYER TO A THIRD PARTY ON ACCOUNT OF SUCH LOSS OR FOR ANY LABOR OR ANY OTHER EXPENSE DAMAGE OR LOSS OCCASIONED BY SUCH PRODUCT INCLUDING PERSONAL INJURY OR...

Page 4: ...any and all claims demands actions causes of action and or suits in law or equity now existing or hereafter arising whether known or unknown against Seller and Luminex and their respective officers d...

Page 5: ...sale of Luminex instrumentation to you the purchaser you may obtain a license under Luminex s patents if any to use this unit of Luminex instrumentation with fluorescently labeled microsphere beads au...

Page 6: ...RMITTED BY APPLICABLE LAW LUMINEX AND ITS SUPPLIERS DISCLAIM ALL WARRANTIES EITHER EXPRESS OR IMPLIED INCLUDING BUT NOT LIMITED TO IMPLIED WARRANTIES OF MERCHANTABILITY FITNESS FOR A PARTICULAR PURPOS...

Page 7: ...Shipment 6 The System 7 Theory of Operation 7 Hardware 8 xMAP Technology Reagents 8 Required Laboratory Reagents 9 Luminex Software 9 Luminex 200 Performance Specifications 9 Speed 9 Accuracy and Pre...

Page 8: ...ake Filter 22 Luminex XYP Instrument Air Intake Filter 23 Syringe Seal 24 Luminex 200 Analyzer Ventilation Filter 26 Annually 27 Sheath Filter 27 As required 28 Fuses 28 Replacing the SD System with a...

Page 9: ...iii Luminex 200 System User Manual Hardware 47 Software 48 xMAP Reagents 49...

Page 10: ......

Page 11: ...signal reader unit raw data storage mechanisms data acquisition software and software to process detected signals Warnings and Notes Informational notes and warnings appear in this manual Note A note...

Page 12: ...ard laboratory safety practices The protection provided by the equipment may be impaired or the warranty voided if the system is used in a manner not specified by the instructions or by Luminex Corpor...

Page 13: ...strong electromagnetic radiation for example unshielded intentional RF sources as these may interfere with proper operation Warning Always handle the Luminex 200 system according to Luminex instructi...

Page 14: ...urn power to the Luminex 200 analyzer OFF and disconnect the power cord All laser apertures are located within the Luminex 200 analyzer and are contained within a protective housing Barcode Reader Las...

Page 15: ...d fingers away from the Luminex XYP instrument slot during operation Warning During operation this system contains exposed moving parts which could result in pinch point hazard Risk of personal injury...

Page 16: ...er 4 Sanitize the system using the Sanitize command in the Luminex software Follow this by washing twice with distilled water 5 Disconnect the system from AC power by turning off the power switch on t...

Page 17: ...Fluid by the second fluidics path This process removes residual sample within the tubing valves and probe The second fluidics path is driven by positive air pressure and supplies sheath fluid to the...

Page 18: ...analysis of the xMAP microspheres as they are processed through the Luminex 200 analyzer Results of the analyses are processed and provided in a report format Hardware The Luminex 200 system includes...

Page 19: ...s prohibited and may result in improper operation of the system Luminex 200 Performance Specifications Speed USB communications link for fast data transfer Automatic transfer of assay templates and ne...

Page 20: ...ue xMAP microsphere sets in a single sample Detect and distinguish surface reporter fluorescence emissions at 575 nm on the surface of 1 100 unique xMAP microspheres sets in a single sample Sample cor...

Page 21: ...ange 100 120 V 10 1 4 Amp and 200 240 V 10 0 8 Amp 47 63 Hz AC inlet fuse 3 Amp 250 V fast acting Optics Reporter laser 532 nm nominal output 10 15 mW maximum 500 mW frequency doubled diode mode of op...

Page 22: ...o 86 F Humidity 20 to 80 noncondensing Altitude designed to operate at up to 2400m 7874 feet above mean sea level Physical dimensions 20 cm 8 inches W x 30 cm 11 75 inches D x 24 75 cm 9 75 inches H W...

Page 23: ...number 08849 00 Operating frequency 55 kHz or equivalent System Overview The system consists of three subsystems electronic fluidic and optical The following section describes the user accessible comp...

Page 24: ...s fitting when you remove the sample probe See Figure 2 5 7 4 3 2 1 6 1 Air intake filter access door 5 Communication Ports DB9 2 Power Switch 6 XYP Communication Port DB9 3 Power Input Module 7 Analy...

Page 25: ...ess doors Two of the access doors are on the front and the third is on the back The front left access door supplies access to the sheath filter The front center access door supplies access to the syri...

Page 26: ...the SD system sheath fluid levels must be monitored manually Check the sheath fluid level before starting a run or procedure Waste Fluid Container The waste fluid container receives waste from the sys...

Page 27: ...ces Daily Maintenance If the system is powered on but idle for more than four hours click Warmup Wait 30 minutes for the Luminex 200 analyzer and the optics system to warm up Before Running Samples 1...

Page 28: ...elected well For a round bottom U bottom plate stack two of the smaller 3 35 mm diameter alignment discs in the selected well For a plate with conical wells place one alignment sphere into the selecte...

Page 29: ...o become pressurized causing pressure errors in the system If the seal gets wet let it air dry Touching the seal can contaminate it If it gets dirty you should replace the waste bottle cap If you are...

Page 30: ...intake filter for buildup of dust Check the SD system and its connection for leaks If you see a leak turn off the power to the Luminex SD system and contact Luminex Corporation Clean the Sample Probe...

Page 31: ...D system 2 Wipe all exterior surfaces with mild detergent then a 10 to 20 household bleach solution and finally with plain distilled water 3 Open both doors of the analyzer Clean all accessible surfac...

Page 32: ...backflushes 3 drains 2 alcohol flushes and 3 washes with distilled water Every Six Months Luminex 200 Analyzer Air Intake Filter To replace the analyzer air intake filter 1 Disconnect the Luminex 200...

Page 33: ...on the Luminex 200 analyzer Luminex XYP Instrument Air Intake Filter To replace the XYP instrument air intake filter 1 Disconnect the Luminex XYP instrument from AC power by turning off the power swit...

Page 34: ...Removing and Replacing the Filter 4 Plug in and turn on Luminex XYP instrument power Syringe Seal To replace the syringe plunger seal 1 Turn the power switch on the back of the analyzer to the off po...

Page 35: ...soon as it begins traveling upward turn off the analyzer CAUTION Do not turn off the analyzer as the arm is going down The syringe pump valve will not be in the correct position allowing sheath fluid...

Page 36: ...ea 6 When the prime finishes close the analyzer door Luminex 200 Analyzer Ventilation Filter To clean the Luminex 200 analyzer ventilation filter 1 Disconnect the Luminex 200 analyzer from AC power by...

Page 37: ...yzer from AC power by turning off the power switch on the rear of the analyzer then unplugging the analyzer power cord from the wall source 2 Disconnect the sheath fluid tubing before changing the fil...

Page 38: ...the power cord from the analyzer or instrument 2 With a small flathead screwdriver open the module door on the lower left corner of the back of the analyzer or instrument See Figure 9 Figure 9 Opening...

Page 39: ...Warm Up via the software if the compressor has turned off 8 When the pressure has stabilized note the Sheath pressure 9 If the pressure is equal to the value noted in step 2 within 0 1psi then the se...

Page 40: ...at the instruments are responding correctly The light above the sample probe on the Luminex 200 and the light next to the door of the XY platform are lit The compressor starts in the Luminex 200 It is...

Page 41: ...isopropanol or 70 ethanol Wash twice with distilled water Shut down Initials for each item listed at the left fill in your initials under each date that you perform the item Sanitize 10 to 20 househo...

Page 42: ......

Page 43: ...he following topics Power Supply Problems Communication Pressurization Fluid Leaks Sample Probe Calibration Problems Acquisition Problems Bead Detail Irregularities Printing Errors Verification Lumine...

Page 44: ...control sample acquisition session uploading and start stop and pause features Symptom Possible problem Solution Analyzer will not turn on or XYP will not turn on The power cord is disconnected Verif...

Page 45: ...there is fluid on the surface on which the system sits The compressor does not engage Run a Prime command If you do not hear the compressor turn on contact Technical Support The Cheminert fitting is...

Page 46: ...24 The syringe valve leaks Hand tighten the syringe connection silver knob on the syringe valve Run a Prime command If leaks continue contact Technical Support Large amount of fluid pooled around ins...

Page 47: ...Sample arm is stuck in the down position The sample probe height is too low or the path to the well is blocked DO NOT turn off the Luminex XYP instrument 1 Remove the blue light housing from the analy...

Page 48: ...libration is slow or fails The calibration microspheres are not fully suspended Vortex the calibration vials to resuspend the microspheres Wrong lot number or target values are entered in Update CAL T...

Page 49: ...are evident on the report contact Technical Support Zero events collected during calibration There is a problem with fluid levels Check the sheath and waste fluid levels Verify that tubing for both b...

Page 50: ...microspheres have been diluted Do not dilute the control microspheres The sample probe height is incorrect Adjust the sample probe height See page 18 The sample probe is clogged Clean the sample probe...

Page 51: ...for the calibration microspheres Ensure that the correct wells are selected in the Maintenance tab The calibration lot number or target values selected in setup is incorrect Enter the correct calibrat...

Page 52: ...problem is analyzer related If Calibration and Controls are successful contact the kit manufacturer A normal bead detail display is shown below It depicts a tight bead population within a white region...

Page 53: ...il above looks like this Figure 11 Normal Histogram Symptom Possible problem Solution xMAP microspheres classify too high You may be using photobleached calibration microspheres Replace the calibratio...

Page 54: ...sure there is sheath fluid in the sheath container Prime the system until all air is out of the system Microspheres appear as a long diagonal line The xMAP microspheres have agglutinated Add additiona...

Page 55: ...Luminex Technical Support Draining the Reservoir If you need to ship the Luminex SD system back to Luminex Corporation drain the reservoir before you pack the system 1 Perform a warmup to pressurize...

Page 56: ...Luminex 200 System User Manual 46...

Page 57: ...Fast Acting Fuse Qty 10 CN 0019 01 3 Amp 250 Volts Fast Acting Fuse CN 0051 01 Heater Block XYP CN 0017 01 Luminex 200 System with IS 2 3 LX200 IS2 3 Luminex 200 System with IS 2 3 Developer s Workben...

Page 58: ...servoir XYP CN 0022 01 Sample Needle Height Alignment Kit Sample Probe Alignment Kit CN 0015 01 Short Sample Needle CN 0006 01 Long Sample Needle CN 0007 01 Sample Holder Large 1 5 mL CN 0008 01 Sampl...

Page 59: ...ontrol xMAP Classification Control L100 CON1 Microspheres LX100 RP1 Control xMAP Reporter Control L100 CON2 Microspheres MagPlex DD CL1 CL2 Calibrator 5mL MagPlex Classification Calibrator Microsphere...

Page 60: ...Luminex 200 System User Manual 50...

Page 61: ...g beam into people s eyes 4 staring into beam 4 B barcode label 13 biohazard 2 biological 1 warning 5 blue indicator light 1 5 C calibration 21 capacity specifications 10 caution 2 CD supplied 9 chemi...

Page 62: ...pecifications 12 M maintenance schedule annual 27 as required 28 daily 17 monthly 21 routine tasks 19 semi annual 22 weekly 20 manual adjustment 16 mechanical 1 5 monitor waste fluid refill sheath con...

Page 63: ...yzer 11 Luminex 100 IS 9 11 13 Luminex XYP 12 PC 12 reporter laser 11 sensitivity 10 speed 9 vortex 13 speed specifications 9 start up procedure 17 status of instrument 5 subsystems 13 surge protector...

Page 64: ......

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