50
www.logosbio.com
Note
: To get the best focus, the image can be magnified by using the “Zoom-in” button located under
the “Count” button. Initially, the “Zoom-in” button is set to a 1x image. When pressed once, a 2x image
appears. This 2x image may be the best zoom-in status for focusing. When pressed again, a 4x image
will be shown. Pressing the button again takes the screen back to a 1x image.
Note
: Check if all cells in the preview screen are immobile. If some cells are still moving, wait for all
cells in the preview screen to stop moving.
4.3.5 Press “BF/GF/RF selection button” once to preview the green fluorescence (GF) image. Increase
the GF intensity value if the preview image is too dim so that only some cells are visualized. Decrease
the GF intensity value if the preview image is too bright so that background fluorescence starts to come
up. The optimal GF intensity value should be determined empirically. The factory set value for
mammalian cells is 5.
Note
: During the preview step, if cells are irradiated for a long period of time, their fluorescent intensities
will quickly decrease. This bleaching phenomenon is accelerated when a higher level of fluorescence
is used. Always try to use the shortest preview time and the lowest fluorescence level when the GF or
RF preview is selected.
<Green Fluorescence Mode in Fluorescence Counting>