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Leica RM2255

Rotary Microtome

Instruction Manual

Leica RM 2255
V1.4 English - 02/2007
Always keep this manual with the instrument.
Read carefully before working with the instrument.

Summary of Contents for RM2255

Page 1: ...Leica RM2255 Rotary Microtome Instruction Manual Leica RM2255 V1 4 English 02 2007 Always keep this manual with the instrument Read carefully before working with the instrument...

Page 2: ......

Page 3: ...details of the present manual are not to be considered as warranted characteristics of our products These are determined only by the contract provisions agreed between ourselves and our customers Leic...

Page 4: ...Assembling the handwheel 18 4 5 Electrical connections 18 4 6 Switching on the instrument 21 5 Operation 22 5 1 Operating elements and their functions 22 5 1 1 Instrument control panel 22 5 1 2 Contro...

Page 5: ...e 50 6 3 2 Knife holder E E TC 51 6 3 3 Knife holder N NZ 54 6 4 Section waste tray 56 6 5 Backlighting 56 6 6 Tray 57 6 7 Freezer pack 57 6 8 Universal microscope carrier 58 6 9 Magnifying lens 60 6...

Page 6: ...n the text and their meanings 5 1 4 Instrument type All information provided in this manual applies only to the instrument type indicated on the cover page An identification label indicating the instr...

Page 7: ...the safety regulations for electrical measuring control regulating and laboratory devices In order to maintain this condition and ensure safe operation the operator must observe all the instructions...

Page 8: ...ing of the instrument upon arrival Severe damage can be caused to the instrument if the voltage selector is set to an incorrect voltage When changing the voltage selector setting ensure that the instr...

Page 9: ...action prior to the next section the block will advance by the retraction value PLUS the selected section thickness This may cause damage to both speci men and knife The handle of the handwheel must a...

Page 10: ...al position Emergency stop function The emergency stop function is activated with the red EMERGENCY STOP switch 1 on the upper left of the front of the microtome The sectioning motor stops immediately...

Page 11: ...red foldaway handle To cover the cutting edge fold the knife guard handle 9 upwards as illus trated in Fig 6 For the new knife holder E the two clamping levers 10 11 must always remain in the positio...

Page 12: ...Lever for activating the handwheel brake Handwheel locking mechanism Separate control panel Directional specimen holder fixture with quick clamping system Fig 7 Knife holder base Knife holder E with...

Page 13: ...el lock All controls and LEDs are centralized in a separate control panel All control elements are logically arranged in functional groups and easily identifiable The specimen retraction can be turned...

Page 14: ...nge during storage 5 C to 55 C Relative humidity max 80 non condensing Humidity during storage 80 according to IEC 1010 UL 3101 EN 61010 Dimensions and weight Basic instrument Width including handwhee...

Page 15: ...in 10 0 m increments from 100 0 600 0 m in 50 0 m increments Object feed 28 mm 1 mm feed motion via step motor Vertical stroke 70 mm Maximum sectioning area w o retraction 65 mm without specimen orie...

Page 16: ...ong 0170 11568 1 bottle 50 ml of oil for drives type 405 0336 06086 2 micro fuses 3 2 AT 6943 03201 1 Leica brush w magnet 0183 40426 1 dust protective cover 0212 30350 1 operating manual G E F S 0700...

Page 17: ...r the cover Lift the instrument 5 by holding it by the base plate and the recessed grip on the back see Fig 10 and lift it out of the formed cushion 6 4 Startup 4 3 Unpacking and installation Fig 9 7...

Page 18: ...grounded power socket Use only the power cable provided that matches the power supply outlets of the country of use Do not use an extension cable without a ground wire Before connecting the instrument...

Page 19: ...es audible click 21 26 Connecting the power supply Before connecting the power cable make sure that the power switch 27 on the rear of the instrument is switched to O OFF Various country specific powe...

Page 20: ...the two screws 17 Connecting the dummy plug Insert the dummy plug provided 18 into the connecting jack 19 on the rear of the in strument and fasten it by tightening the screws 20 Connecting the foot s...

Page 21: ...the last value set for the section thickness or trimming thickness depending on which setting was last activated This is indicated simultaneously on the control panel and microtome The LED of the act...

Page 22: ...ckness trim ming section thickness Four digit display of the section counter MENU MODE CLEAR pressed simultaneously switches to the setting of the retraction value Green LED Section thickness sum indi...

Page 23: ...d trimming mode CUT MODE button Mode selection Button Set sectioning window Yellow LED Flashes during coarse feed backwards lights up when rear end position is reached Yellow LED Flashes during coarse...

Page 24: ...n 5 0 m increments from 60 0 100 0 m in 10 0 m increments Trimming section thickness setting range 1 600 m Setting values from 1 0 10 0 m in 1 0 m increments from 10 0 20 0 m in 2 0 m increments from...

Page 25: ...rd and the LED stops flashing and remains lit When continuous feed is selected the coarse feed buttons have the same func tions as in trimming mode The STEP function is useful for careful step by step...

Page 26: ...e position In motorized sectioning mode the retraction depends on the setting of the sectioning speed In manual mode the retraction value can be selected in 5 m increments between 5 and 100 m Specimen...

Page 27: ...l To exit the retraction settings press MENU MODE A retraction movement takes place by the newly selected value after each section While the specimen is in retraction the yellow LED on the RETRACT dis...

Page 28: ...s turned off Turn the handwheel to position the lower edge of the sample approxi mately 3 mm above the cutting edge Press the SET SECTIONING WINDOW button This defines the first window border The gree...

Page 29: ...rocking mode Rock Start and stop motorized sectioning To start motorized sectioning press the RUN STOP and ENABLE buttons simultaneously after selecting the desired operating mode While the cutting m...

Page 30: ...erating mode Select SINGLE operating mode After starting sectioning a single sectioning stroke is completed The specimen then stops automatically in the upper end position of the vertical stroke In mo...

Page 31: ...d sectioning The yellow LED 41 in the COARSE FEED button lights up When the front end position is reached the sectioning process stops automatically Upon restart no more feed motion takes place You ca...

Page 32: ...ch again to stop it The specimen stops in the next upper end position SINGLE single stroke operating mode Press the foot switch once briefly to start motorized sectioning After every step the specimen...

Page 33: ...pecimen by 2 A total of 4 complete turns 8 are possible in every direction The accuracy is approximately 0 5 For ease of estimation there is a white marking on the handle and a notch point that is not...

Page 34: ...e object head moves i e it is raised or low ered fine adjustment is necessary Failure to adjust the force balance may result in injury while working The force balance is adjusted using the screw 34 wh...

Page 35: ...e right side of the microtome base plate This enables faster and better positioning of the knife holder at the specimen if various combinations of standard specimens and specimen holders are used The...

Page 36: ...men clamps for more information see Chapter 6 Optional accessories To do so proceed as follows Move the specimen holder fixture 60 to the upper end position by turning the handwheel 12 and engage the...

Page 37: ...1 until the knife holder 57 can be moved Move the knife holder until the index mark of the desired clearance angle coincides with the reference line on the knife holder base Example The enlarged detai...

Page 38: ...t a specimen block into the specimen clamp A detailed description for inserting the specimen into various specimen clamps and specimen holders is provided in Chapter 6 Optional accessories 5 6 Clampin...

Page 39: ...ct the CONT operating mode continuous stoke Deactivate the handwheel lock and release the brake Start motorized sectioning and trim the sample Terminate trimming when the desired sectioning surface an...

Page 40: ...t the sectioning speed according to the hardness of the specimen For hard specimens always select a slow speed Check the sectioning speed setting and set an appropriate speed Start motorized sectionin...

Page 41: ...the side and briefly tighten it with an Allen key size 4 Remove the rubber ring only after attaching the object head Fig 27 1 2 4 5 3 6 1 2 Directional fixture for specimen clamps Loosen the eccentri...

Page 42: ...the baseplate 9 Fig 29 10 11 Using the 4 supplied screws 11 and the Allen key size 3 fasten the baseplate to the object head 12 11 Now screw the fine directional fixture for specimen clamps with the...

Page 43: ...tem It is used as specimen holder for use with the fine directional fixture for specimen clamps with zero point indicators or the directional fix ture for specimen clamps Screw the 4 screws 13 into bo...

Page 44: ...2 Vee insert The vee insert 70 is mounted in the hole pro vided in the lower movable jaw of the standard specimen clamp This makes it possible to clamp round speci mens in the standard specimen clamp...

Page 45: ...te both for clamping very small foil pieces and flat angular samples It is mounted in the standard specimen clamp Clamping of flat angular samples To clamp angular samples replace the long set screw 7...

Page 46: ...d then the other two screws 81 with the Allen key 71 Place the foil clamp 82 in the standard specimen clamp so that the beveled surface 80 on the back of the foil clamp points to the right or left Tur...

Page 47: ...commercial cassettes Push the lever 60 forwards Mount the cassette 65 horizontally or vertically as required To clamp the cassette release the lever 60 6 2 5 Universal cassette clamp Use the ice coole...

Page 48: ...wise To orient the inserted sample insert the pin 92 into the bore 93 and rotate it counter clockwise to release the clamp You can now rotate the specimen so that the side you want faces upwards To lo...

Page 49: ...d 3 from the front and tighten the screws 2 with an Allen key size 3 Next insert the screw 1 from the side and briefly tighten it with an Allen key size 4 Insert the super mega cassette clamp from the...

Page 50: ...50 on the right side of the microtome base plate counterclockwise Reposition the knife holder together with the knife holder base forward or backward as appropriate Secure the clamping mechanism by ro...

Page 51: ...conventional disposable blades from all current manufac turers It is available in two models one for narrow band blades and one for broad band blades The knife holder has a lateral movement so that t...

Page 52: ...ed to replace the clamp mount including the pertinent clamping lever Leica Biosystems offers special prices for new clamp mounts in case of damages to the clamp mount after the warranty has expired In...

Page 53: ...nd 11 must remain in the position shown at all times as otherwise isolated malfunctions of the knife holder can occur Clamping lever for the blade 10 at the right clamping lever for the lateral displa...

Page 54: ...knife guard 8 to the center Set the knife support bar 46 onto the height adjustment screws not visible in the position shown The flat ends of the height adjustment screws must be located in the slots...

Page 55: ...sible in the actual center of rotation of the knife holder The lay on edge 39 of the rear clamping chucks serves as a reference position for correct knife height adjustment The knife edge should be pa...

Page 56: ...acklighting to the socket 5 on the microtome The backlighting illuminates once the micro tome is turned on with the mains switch NEVER use the backlighting illumina tion with the super mega cassette c...

Page 57: ...n the hood of the microtome in the same manner as the tray or it can be placed next to the instrument When using the freezer pack on the instrument the tray Fig 47 must be removed Remove the freezer p...

Page 58: ...or RM2265 6 Allen key size 3 7 4 countersunk screws to install support plate 8 Allen key size 8 Unpack all accessories in the package and check for completeness Assembling the universal microscope car...

Page 59: ...plate so that the front feet of the microtome are positioned in the shallow recesses A 3 Attach horizontal arm Slide the support ring e onto the vertical column and position it so that the lock nut f...

Page 60: ...er optic light guide to be installed Adjust the position of the magnifying lens to the specimen being processed The magnifying lens can be swiveled com pletely to the side if necessary 1 3 The magnify...

Page 61: ...connect ing jack on the rear of the device Plug the power plug into the power socket Carefully read the instruction manual provided separately before use Attach the fiber optic light guide 2 to the a...

Page 62: ...f tray RM22xx 0502 38961 Knife holder E broad band Run off tray RM22xx 0502 39114 Knife holder E TC RM22xx silver 0502 37997 Standard specimen clamp with adapter 50x60 silver 0502 38005 Standard speci...

Page 63: ...100X 100 120V 50 60Hz 0502 30214 Leica CLS 100X 230V 50 60Hz 0502 30215 Leica CLS 100X 240V 50 60Hz 0502 30216 Fiber optic light guide 0502 30028 Backlighting assembly 0502 38719 Foot switch 05023825...

Page 64: ...ting Following is a list of the most common problems which can arise while working with the instrument along with possible causes and troubleshooting procedures If the malfunction cannot be remedied w...

Page 65: ...Foot switch was held down too long when starting the section ing process 2 The RUN STOP and ENABLE buttons of the control panel were not released simulta neously after starting 1 STEP mode is still ac...

Page 66: ...essary tighten the levers and screws Adjust the sectioning speed and or section thickness when sectioning Select a thinner section Problem Corrective action Possible cause Insufficient clamping of bla...

Page 67: ...lock Switch the unit off and unplug it Remove the blade from the knife holder and insert it in the receptacle at the bottom of the dispenser or remove the knife from the knife holder and put it back i...

Page 68: ...pressure plate 83 Clean all parts of the knife holder If several knife holders are cleaned at the same time the parts must not be mixed up For cleaning and removal of paraffin do not use xylene or cl...

Page 69: ...ping lever 60 see also chapter 8 2 3 8 2 Maintenance Fig 68 60 13 Switch the unit off and unplug it Use only the supplied replacement fuses Both fuses must have the same rating check the imprint Inser...

Page 70: ...ed by Leica 3 Have the instrument inspected on a regular basis by a qualified service technician au thorized by Leica The intervals depend on how heavily the instrument is used 1 Thoroughly clean the...

Page 71: ...l displacement Clamping lever 10 for the blade Fig 70 Knife holders N and NZ Sliding surfaces of the finger guard 8 Knurled nuts 73 for measuring height ad justment 95 96 55 8 2 3 Lubricating the inst...

Page 72: ...ization or the or ganization from which you have purchased the contractual product shall apply exclusively Service information If you are in need of technical customer service or spare parts please co...

Page 73: ...armonized standards were applied EN 61010 2 101 2002 Safety requirements for electrical equipment for measurement control and laboratory use Part 2 101 Particular requirements for in vitro diagnostic...

Page 74: ...74 Instruction manual V 1 4 02 2007 People s Republik of China...

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