Leica RM2245 Instruction Manual Download Page 1

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/HLFD50

Rotary Microtome

Instruction Manual

Leica RM2245
V1.6 Rev B, English – 10/2009
Always keep this manual with the instrument.
Read carefully before working with the instrument.

Summary of Contents for RM2245

Page 1: ...HLFD 50 Rotary Microtome Instruction Manual Leica RM2245 V1 6 Rev B English 10 2009 Always keep this manual with the instrument Read carefully before working with the instrument...

Page 2: ......

Page 3: ...details of the present manual are not to be con sidered as warranted characteristics of our products These are determined only by the contract provisions agreed between ourselves and our customers Lei...

Page 4: ...ectrical connections 19 4 6 Switching on the instrument 21 5 Operation 22 5 1 Operating elements and their functions 22 5 1 1 Instrument control panel 22 5 1 2 Control panel 23 5 1 3 Display and contr...

Page 5: ...ife holder N NZ 51 6 4 Blades knives 53 6 4 1 Disposable blades 53 6 4 2 Knives 53 6 5 Section waste tray 55 6 6 Backlighting 55 6 7 Tray 56 6 8 Freezer pack 56 6 9 Universal microscope carrier 57 6 1...

Page 6: ...ny other use of the instrument is considered improper 1 4 Instrument type All information provided in this manual applies only to the instrument type indicated on the cover page An identification labe...

Page 7: ...d warnings contained in this instruction manual For current information about applicable stand ards please refer to the CE declaration of conformity on our Internet site www leica microsystems com The...

Page 8: ...mplies with the local power requirements of your laboratory The power cable inlet is closed with an adhesive tape that indicates the actual voltage setting of the instrument upon arrival Severe damage...

Page 9: ...rient the specimen during the retraction phase If a block is oriented during retraction the block will advance by the retraction value PLUS the selected section thickness before the next section This...

Page 10: ...brake is applied correctly If the lever is moved beyond this point it is possible that the handwheel is no longer braked To unlock the handwheel brake turn the lever 3 back to its original position Po...

Page 11: ...guard to the center Knife holder E E TC The knife guard on knife holder E E TC consists of a red foldaway handle 9 To cover the cutting edge fold the knife guard handle 9 upwards as illustrated in Fi...

Page 12: ...rd on the knife holder Smooth turning handwheel Lever of handwheel brake Handwheel Locking mechanism Separate control panel Directional specimen holder fixture with quick clamping system Fig 6 Knife h...

Page 13: ...f the instrument such as retraction locking trim ming thickness section thickness and counters for the total number of sections and total thick ness sectioned is displayed directly on the in strument...

Page 14: ...ing storage 5 C to 55 C Relative humidity max 80 non condensing Humidity during storage 80 according to IEC 1010 UL 3101 EN 61010 Dimensions and weight Basic instrument Width including handwheel 413 m...

Page 15: ...m in 5 0 m increments from 50 0 100 0 m in 10 0 m increments from100 0 600 0 m in 50 0 m increments Object feed 28 mm 1 mm feed motion via step motor Vertical stroke 70 mm Maximum sectioning area w o...

Page 16: ...ewdriver 3x50 186 long 14 0170 11568 1 bottle 50ml of oil for drives type 405 14 0336 06086 2 micro fuses 1 0 AT 14 6943 01001 1 brush w magnet 14 0183 40426 1 dust protective cover 14 0212 30350 1 In...

Page 17: ...nsport Note this on the shipping documents and check the shipment for possible damage Fig 8 Fig 9 1 1 1 1 1 2 2 2 2 2 3 3 3 3 3 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 5 5 5 5 5 Loosen and unscrew the...

Page 18: ...rument on the back and lift it out of the formed cush ion 8 Never hold the instrument for transport by the handwheel or the rotary knob for section thickness adjustment Observe the correct resting ang...

Page 19: ...a grounded power socket Use only the power cable pro vided that matches the power supply outlets of the country of use Do not use an extension cable Before connecting the instrument to the power suppl...

Page 20: ...voltage selector block 24 white and insert it again such that the correct local voltage is displayed in the viewing window 22 Reinsert the voltage selector housing with the block and fuses and push i...

Page 21: ...on with the power switch do not press any of the buttons of the control panel Turn the instrument on with the power switch at the rear This is followed by a beep The instrument initializes The display...

Page 22: ...it display for displaying section thickness trim ming section thickness Four digit display of the section counter MENU MODE CLEAR pressed simultaneously switches to the setting of the retraction value...

Page 23: ...switching between sectioning mode and trimming mode Yellow LED Flashes during coarse feed backwards lights up when rear end position is reached Yellow LED Flashes during coarse feed forwards lights up...

Page 24: ...kness trimming section thickness Adjust these settings using the keys on the control panel Section thickness setting range 0 50 m 100 m Setting values from 0 5 m 5 0 m in 0 5 m increments from 5 0 m 2...

Page 25: ...n a single step is also effected in the appropriate direction Longer activation of the double arrow coarse feed button effects a repeated feed motion for as long as the button is pressed 5 Operation I...

Page 26: ...f all previously completed sections Fig 17 Press the button to start the slow backwards movement The travel continues as long as the button is held depressed Press the appropriate button to start a ra...

Page 27: ...e retraction settings press MENU MODE A retraction movement takes place by the newly selected value after each section While the specimen is in retraction the yellow LED on the RETRACT display 4 light...

Page 28: ...he COARSE FEED button lights up Fig 16 Feed stops once the front end position has been reached further sectioning is no longer possible in that position You can continue to work on the specimen by pre...

Page 29: ...specimen by 2 A total of 4 complete turns 8 are possible in every direction The accu racy is approximately 0 5 For ease of estimation there is a white mark on the handle and a notch point that is noti...

Page 30: ...If the specimen head remains in this exact posi tion the setting is correct If the specimen head moves i e it is raised or lowered fine adjustment is necessary Failure to adjust the force balance may...

Page 31: ...right side of the microtome base plate This enables faster and better positioning of the knife holder at the specimen if various combinations of standard specimens and specimen holders are used The r...

Page 32: ...head 60 to the upper end position by turning the handwheel 12 and engage the handwheel lock To release the clamping system turn the screw 61 of the quick clamping system 64 counterclockwise using an A...

Page 33: ...len key size 4 71 until the knife holder 57 can be moved Move the knife holder until the index mark of the desired clearance angle coincides with the reference line on the knife holder base Example En...

Page 34: ...tion Activate the handwheel lock by allowing the handwheel handle to lock in place and then activate the brake Insert a specimen block into the specimen clamp A detailed description for inserting the...

Page 35: ...lamping lever 10 downwards counterclockwise The ejector 9a ensures safe blade changing Use the blade ejector to eject the blade Once the blade has been removed from the blade holder it is disposed of...

Page 36: ...e rotation speed of the handwheel must be adapted to suit the hardness of the specimen For harder specimens use a slower speed Always use a different area of the cutting edge for trimming and sectioni...

Page 37: ...eration 5 10 Finishing daily routine Raise the specimen to the upper end position by turning the handwheel and engage the handwheel lock Always remove the knife blade before detaching the knife holder...

Page 38: ...iefly tighten it with an Allen key size 4 Remove the rubber ring only after attaching the specimen head Fig 28 1 2 4 5 3 6 1 2 Directional fixture for specimen clamps Loosen the eccentric bolt 6 by tu...

Page 39: ...clamps from the baseplate 9 Fig 30 10 11 Using the 4 supplied screws 11 and the Allen key size 3 fasten the baseplate to the specimen head 12 11 Now screw the fine directional fixture for specimen cla...

Page 40: ...ed as specimen holder for use with the fine directional fixture for specimen clamps with zero point indicators or the directional fixture for specimen clamps Screw the 4 screws 13 into bore A with an...

Page 41: ...holders 6 2 2 Vee insert The vee insert 70 is mounted in the hole pro vided in the lower movable jaw of the standard specimen clamp This makes it possible to clamp round speci mens in the standard sp...

Page 42: ...ropriate both for clamping very small foil pieces and flat angular specimens It is mounted in the standard speci men clamp Clamping of flat angular specimens To clamp angular specimens replace the lon...

Page 43: ...e other two screws 81 with the Allen key 71 Place the foil clamp 82 in the standard specimen clamp so that the beveled surface 80 on the back of the foil clamp points to the right or left Turn the knu...

Page 44: ...5 horizontally or verti cally as required To clamp the cassette release the lever 60 6 2 5 Universal cassette clamp 60 Order No 0502 37999 Use the ice cooled UCC with the non directional specimen hold...

Page 45: ...nd tighten the screws 2 with an Allen key size 3 Next insert the screw 1 from the side and briefly tighten it with an Allen key size 4 Insert the super mega cassette clamp from the side on the left in...

Page 46: ...ise To orient the inserted specimen insert the pin 92 into the bore 93 and rotate it coun terclockwise to release the clamp You can now rotate the specimen so that the side you want faces upwards To l...

Page 47: ...ht side of the microtome base plate counterclockwise Reposition the knife holder together with the knife holder base forward or backward as appropriate Secure the clamping mechanism by rotating the le...

Page 48: ...ned for conventional disposable blades from all current manufactur ers It is available in two models one for narrow band blades and one for broad band blades The knife holder has a lateral displacemen...

Page 49: ...replace the clamp mount including the pertinent clamping lever Leica Biosystems offers special prices for new clamp mounts in case of damages to the clamp mount after the warranty has expired In this...

Page 50: ...vers 10 and 11 must remain in the position shown at all times as otherwise isolated malfunctions of the knife holder can occur Clamping lever for the blade 10 at the right clamping lever for the later...

Page 51: ...d 8 to the center Set the knife support bar 46 onto the height adjustment screws not vis ible in the position shown The flat ends of the height adjustment screws must be located in the slots at each e...

Page 52: ...ly as possible in the actual center of rotation of the knife holder The lay on edge 39 of the rear clamping chucks serves as a reference position for correct knife height adjustment The knife edge sho...

Page 53: ...m high 01 Packages of 50 pcs 14 0358 38926 10 Packages of 50 pcs 14 0358 38383 Fig 49 Fig 50 6 4 2 Knives Knife 16cm profile c steel Knife 16cm long profile c ground on both sides for paraffin and fro...

Page 54: ...216 07130 Knife 16 cm profile d tungsten carbide Knife 16 cm long tungsten carbide profile d Note Knife case 14 0213 11140 included Order No 14 0216 04813 Knife 16 cm profile c tungsten carbide Knife...

Page 55: ...g to the socket 5 on the microtome The backlighting illuminates once the micro tome is turned on with the power switch NEVER use the backlighting illumination with the super mega cassette clamp Fig 56...

Page 56: ...speci mens being cooled The insulation jacket can be placed on the hood of the microtome in the same manner as the tray or it can be placed next to the instrument When using the freezer pack on the in...

Page 57: ...plate small for RM2265 6 Allen key size 3 7 4 countersunk screws to install support plate 8 Allen key size 8 Unpack all accessories in the package and check for completeness Assembling the universal m...

Page 58: ...the support ring e onto the vertical col umn and position it so that the lock nut f is facing to the back Tighten the lock nut Slide the cross member d onto the column Ensure that the lock screw g is...

Page 59: ...use a burning glass ef fect Danger of fire Use the provided protective cap 4 to cover the magnifying lens Open the screw 3 on the horizontal arm of the microscope carrier in a counterclock wise direct...

Page 60: ...er cable 1 to the connect ing jack on the rear of the device Plug the power plug into the power socket Carefully read the instruction manual provided separately before use Attach the fiber optic light...

Page 61: ...RM22xx silver 14 0502 37997 Narrow band disposable blade type 819 1x50 14 0358 38925 Wide band disposable blade type 818 1x50 14 0358 38926 Knife 16cm profile C steel 14 0216 07100 Knife 16cm profile...

Page 62: ...holder 14 0355 10405 Special wrench f EM holder 14 0356 10869 Universal microscope carrier complete 14 0502 40580 Magnifying lens assembly 14 0502 42790 Cold light sources Leica CLS 100X 100 120V 50 6...

Page 63: ...the previously entered values are still correct The values may have to be reentered You can continue with your normal working routine If E 1 appears repeatedly when switching the instrument on the li...

Page 64: ...ow the procedure in Item 1 No display no reaction to but tons pressed after the instru ment is switched on 1 The power cable is not connect ed correctly 2 Power fuses are defective 3 The cable of the...

Page 65: ...te of the knife holder 3 Stripes in sections For knife holder E 2 Section compression The sections are very com pressed show folds or are squeezed together Adjust the sectioning speed and or section t...

Page 66: ...e blade before detaching the knife holder from the instrument Always put the knives back into the knife case when not in use Never place a knife anywhere with the cutting edge facing upwards and never...

Page 67: ...otate the blade clamping lever 10 down ward and pull it out sideways Remove pressure plate 83 Clean all parts of the knife holder If several knife holders are cleaned at the same time the parts must n...

Page 68: ...spring of the clamping lever 60 see also chapter 8 2 3 8 Cleaning and Maintenance Fig 74 60 13 Switchtheunitoffandunplugit Useonly the supplied replacement fuses Both fuses must have the same rating...

Page 69: ...wing inspection intervals depending on the general workload of the instru ment as defined in the three categories listed in the table Number of sections per day Workload hours per day Sectioning speed...

Page 70: ...e lateral displacement Clamping lever 10 for the blade Fig 76 Knife holders N and NZ Sliding surfaces of the finger guard 8 Knurled nuts 73 for measuring height adjustment 95 96 55 8 2 3 Lubricating t...

Page 71: ...the or ganization from which you have purchased the contractual product shall apply exclusively Service information If you are in need of technical customer service or spare parts please contact your...

Page 72: ...72 Instruction manual V 1 6 10 2009 Peoples Republic of China...

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