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Leica M Series User Manual

Fluorescence 118

FIM – Fluorescence Intensity Manager

FIM consists of a diaphragm disk with pinhole
diaphragms of varying transmission capaci-
ties. The intensity of the excitation light can
be reduced in five fixed steps. The light can be
changed from 100 % to 55 %, 30 % and 17 %
down to 10 %.

The advantage of this procedure is its absolute
reproducibility and fast switching between the
intensity levels. A separate FIM level can be
stored for every fluorescence filter. Different
intensities of fluorescence can thus be cali-
brated to one another.

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Summary of Contents for M165 FC

Page 1: ...Leica M165 FC Leica M205 FA M205 FCA User Manual ...

Page 2: ...hemicals to clean colored surfaces or accessories with rubberized parts This could damage the surfaces and specimens could be contaminated by abraded particles O O In most cases we can provide special solu tions on request Some products can be modified and we can offer other accesso ries for use in clean rooms Servicing O O Repairs may only be carried out by Leica Microsystems trained service tech...

Page 3: ... of the stereo microscope accessories and electrical accessories as well as general safety instruc tions You can combine individual system articles with articles from external suppliers e g cold light sources etc Please read the user manual and the safety requirements of the supplier Before installing operating or using the instru ments read the user manuals listed above In particular please obser...

Page 4: ...tory to read and observe Failure to comply can cause the following O O Hazards to personnel O O Functional disturbances or damaged instruments Danger due to hot surface This symbol warns against touching accessible hot surfaces e g those of light bulbs Important information This symbol indicates additional infor mation or explanations that intend to provide clarity Explanatory notes O O This symbo...

Page 5: ...are outside of the scope of this manual Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims Place of use O O Refer to Safety Concept booklet O O Electrical components must be placed at least 10 cm away from the wall and from flammable substances O O Avoid large temperature fluctuations direct sunlight and vibrations These condi tions can dist...

Page 6: ...ts O O Refer to Safety Concept booklet Disposal O O Refer to Safety Concept booklet Legal requirements O O Refer to Safety Concept booklet EC Declaration of Conformity O O Refer to Safety Concept booklet Health risks Workplaces with stereo microscopes facilitate and improve the viewing task but they also impose high demands on the eyes and holding muscles of the user Depending on the duration of u...

Page 7: ...ys O O Dummy filter carriers in the free positions of the rapid filter changer prevent direct UV radiation from reaching the eyes O O UV filters are installed in the observation beam paths to protect the eyes O O The stray light protection on the lamp housing prevents irradiation of the hands Warning UV radiation could damage the eyes Therefore O O Never look into the light spot on the speci men p...

Page 8: ...es Danger of explosion Never cover the air duct on the lamp housing Danger of fire Mercury lamp O O Follow the user manual and safety instruc tions provided by the lamp manufacturer and in particular the section on how to proceed if a lamp breaks and releases mercury O O For transport remove the mercury lamp transport it in its original packaging and protect moving parts in the lamp housing using ...

Page 9: ... Objective Nosepiece Adjusting Parfocality 29 Leica FluoCombi III Preparations 30 Leica FluoCombi III Assembly 31 Leica FluoCombi III Adjusting the Parfocality 33 Leica FluoCombi III Centering the Objectives 35 Leica FluoCombi III Filter and UV Protection Screen 36 Transmitted light Base Leica TL BFDF Before First Use 37 Transmitted light Base Leica TL BFDF 38 Leica TL RC Leica TL RCI 39 Leica Iso...

Page 10: ...hototube 50 84 Trinocular Video Phototube 100 85 Trinocular Video Phototube 100 ErgoTube 5 45 86 Microscope Carrier The Objective Nosepiece 88 Objectives and Optical Accessories The Different Types of Objectives 90 Bases Leica TL ST Transmitted light Base Controls 92 Leica TL ST Transmitted light Base Operation 93 Leica TL ST Transmitted light Base Changing Bulbs 94 Leica TL BFDF Transmitted light...

Page 11: ... Iris Aperture 124 Commissioning the Fluorescence System 125 Accessories Leica Hand Wheel and Foot Switch 127 Leica SmartMove 128 Leica SmartTouch 129 Leica PSC Controller 130 Dimensional Drawings Leica M165 FC 132 Leica M205 FA 135 Leica M205 FCA 138 Leica LED5000 MCI 141 Leica LED5000 RL 142 Leica MATS TL 143 Specifications Leica M165 FC Leica M205 FA Leica M205 FCA 145 Leica EL6000 Specificatio...

Page 12: ...Leica M Series User Manual The Leica M Series for Fluorescence 12 The Leica M Series for Fluorescence ...

Page 13: ...s apochromatically corrected with much technological effort Contrast sharpness richness in detail resolution image and color fidelity are opti mum In addition the patented illumination beam path guarantees at every zoom level that light utilization is at a maximum and that fluores cence images are intensely luminous on a jet black background Though the reliability and robustness of Leica stereo mi...

Page 14: ... by the observer becomes distorted a sphere becomes an ellipse a flat surface curves towards the observer Limits are made to be broken The Leica M205 FA is the world s first fluores cence stereo microscope with a zoom range of 20 5 1 This accomplishment however was not enough for Leica s engineers With the new FusionOptic in the Leica M205 FA M205 FCA they have succeeded in going yet another step ...

Page 15: ...ng captures parameters such as the magnifica tion the illumination the position of the iris diaphragm and more and transmits the infor mation to the Leica LAS software Thus you always know the conditions under which an image was acquired Fewer cables A large part of the cables have been routed in the interior of the column Data are also trans ferred via the interface between the column the optics ...

Page 16: ...Modular Design Everything is Relative The Leica M series provides maximum flexibil ity in choosing equipment thanks primarily to the modular configuration and the compatibil ity that Leica has painstakingly maintained for decades The optics carriers eyepieces bases and more can be combined in any way you choose allowing you to create the microscope that best suits your needs Despite this you will ...

Page 17: ...g the specimen in perfect focus If you prefer you can continue to use the previ ous Leica objective series In this case parfocal ity is no longer guaranteed Tubes The interface between the optics carrier and the tube has remained the same so existing tubes fit the new M series The new tubes are designed for eyepieces with field number 23 while the predecessor models were only designed for field nu...

Page 18: ... your Leica consultant click here to skip through the instal lation instructions and go directly to the Quick Start Guide on page 64 If on the other hand you are assembling the microscope yourself continue with the Assembly chapter which begins on page 19 For everything you need to know about the correctuseoffluorescence relatedparts referto page 116 ...

Page 19: ...Leica M Series User Manual Assembly 19 Assembly ...

Page 20: ...nding base Tools used O O Hex socket screwdriver 3 mm Assembling the column adapter 1 Securely install the column adapter on the column using the four included screws Assembling the focusing column 2 Securely screw the focusing column to the base using the six included screws Assembling the Focusing Column for TL Bases ...

Page 21: ...ght base the focus ing column and motorized focus are installed directly on the base no extension plate is required Tools used O O Hex socket screwdriver 3 mm Assembly 1 Place the focusing column on the side 2 Insert the four screws provided into the outer holes of the base 3 Screw the base securely onto the focusing drive ...

Page 22: ...changed Exception If the power fails while the motorized focus is moving the position data are lost In this case the calibration must be repeated using the Leica LAS software or the Leica SmartTouch To do so please consult the respective manual Restricting the bottom travel range 1 Move the motorized focus into the lowest position you want to reach 2 Unscrew the screw of the limit stop on the side...

Page 23: ...m Assembling the optics carrier 1 Place the optics carrier on the focus ing column so that the screw fits into the thread provided and the lug fits into the groove 2 Press the optics carrier backwards to the focusing column and screw it in place using your other hand Optics Carrier ...

Page 24: ...ions 1 Unscrew the positioning screw and remove the protective cover Assembling the tube 2 Push the tube for example the inclined binocular tube into the dovetail ring and rotate it slightly in both directions until the positioning screw meshes with the guide groove 3 While holding the tube only slightly care fully tighten the positioning screw It is automatically brought to the correct posi tion ...

Page 25: ...ilable 10 16 25 and 40 wide field eyepieces for persons wearing glasses Preparation 1 If you want to use an optional graticule insert it now page 80 2 Remove the plastic tube guard Inserting the eyepieces 3 Push the eyepieces into the tubes as far as they will go and check to ensure that they fit tightly and accurately 4 Securely tighten the clamping screws ...

Page 26: ...bly and disassembly so that it does not fall onto the stage plate This applies particu larly to the 2 planapochromatic objective which is very heavy Remove all specimens from the stage plate first 2 Screw the objective clockwise into the optics carrier Alternative fastening options O O If using the objective nosepiece read the instructions on page 28 O O If using the Leica FluoCombi III read the i...

Page 27: ... it is mandatory to install the UV filter and adjust it correctly Always position the UV protection screen so that the operator can never look directly at the light spot Use with one objective 1 Adjust the UV protection screen laterally using the arm 2 Unscrew the hexagon head screw 3 Adjust the UV protection screen using the arm 4 Tighten the hexagon head screw 5 Fasten the UV protection screen u...

Page 28: ...90 and attach the objective nosepiece to the drive hous ing from the front Screw the objective nosepiece firmly into place 3 Unscrew the three Phillips screws on the objective mount of the optics carrier and remove the intermediate ring 4 Screw the optics carrier onto the objective nosepiece 5 Screw both objectives onto the objective nosepiece It makes no difference which position an objective occ...

Page 29: ...paration O O Open the iris diaphragm O O Set the dioptric correction of the eyepieces to 0 Adjustment 1 Rotate the 2 objective into the beam path and set it to the lowest magnification 2 Focus on the specimen 3 Rotate the 1 objective into the beam path 4 Turn the objective on the thread in both directions until the specimen appears sharp 5 Toggle to the 2 objective 6 Select the strongest magnifica...

Page 30: ...ons can result in damage to the micro scope and the connected instruments Preparations 1 Remove the two screws used as transport anchors 2 Screw the two screws into the holes provided for storage In this way you can secure the FluoCombi III for transport whenever necessary 3 Screw the 5 planapochromat into the FluoCombi III 4 Screw the other planapochromatic stereo objective into the FluoCombi III...

Page 31: ...III Assembly 1 Guide the FluoCombi III in from below while holding it flat Ensure that it is aligned with the holder on the focusing column at the left and right 2 Hold the FluoCombi III securely and screw it to the focusing column using the provided screw ...

Page 32: ...he three screws and the adapter ring on the optics carrier 2 Attach the optics carrier to the Fluo Combi III and screw it into place 3 Open the iris diaphragm completely 4 Assemble the tube see page 24 5 Assemble the illuminator see page 50 ff Leica FluoCombi III Assembly Continued ...

Page 33: ...ought into focus again 1 Place a specimen under the microscope 2 Rotate the stereo planachromatic objective under the beam path 3 Reduce the view so that you can see the specimen in the overview 4 Center the specimen under the beam path 5 Adjust the magnification to the highest level and focus on a flat specimen 6 Rotate the 5 planachromat under the beam path 7 Unscrew the clamping screw on the ob...

Page 34: ...ars abso lutely sharp 10 Enlarge the view just enough for the center of the specimen to be visible in the field of view 11 Refocus 12 Tighten the clamping screw see Step 7 13 Rotate the stereo planachromatic objective under the beam path This causes the two objectives to be parfocal This means that from then on you can toggle between the two objectives without refocusing ...

Page 35: ...es The following configuration only has to be carried out once It ensures that the specimen remains in the optical center when the objective is changed To attain optimum results for the follow ing settings insert a graticule into the eyepiece This makes locating the center vastly easier Centering the objective 1 Rotate the 5 planachromat under the beam path 2 Focus on the midpoint of the specimen ...

Page 36: ...dichroic mirror 1 Pull the dummy filter carrier out of the holder 2 Insert the corresponding dichroic mirror Assembling the UV protection screen 1 Screw the UV protection screen to the holder provided on the optics carrier 2 Orient the UV protection screen so that you cannot look directly into the UV light ...

Page 37: ... the transport anchors Before you can use the transmitted light base for the first time it is abso lutely necessary to remove the two transport anchors Transmitted light Base Leica TL BFDF Before First Use Anchor of switching slide Anchor of mirror ...

Page 38: ...echanical stage O O Leica standard stage 10 450 562 The selected stage is mounted on the base before commissioning You can switch between the stages at any time with just a few hand movements The following paragraph assumes use of the base without the stage mounted Disassembly is performed in reverse order of the following steps Standard stage 1 Take the glass plate from the rectangular gap in the...

Page 39: ... The selected stage is mounted on the base before commissioning You can switch between the stages at any time with just a few hand movements The following paragraph assumes use of the base without the stage mounted Disassembly is performed in reverse order of the following steps Standard stage 1 Take the glass plate from the rectangular gap in the standard stage 2 Position the stage on the transmi...

Page 40: ...n the left or the right side of the mechanical stage If the controls are to be mounted on the left hand side the gear rod on the bottom side of the mechanical stage must be unscrewed and reattached in reverse 1 Take the glass plate from the mechanical stage 2 Turn the mechanical stage around and place it onto a non slip surface 3 Change the gear rod from the left to the right hand side 4 Skip the ...

Page 41: ...m the mechanical stage and turn it around 2 Attach the axis with the control buttons to the desired side The fastener snaps into the mechanical stage magnetically 4 Attach the axis with the two supplied hexa gon head screws 5 Attach the cover rail to the mechanical stage Mechanical stage assembly 1 Place the mechanical stage onto the base 2 Pull the upper part of the mechanical stage carefully tow...

Page 42: ...al stage as far as it will go in the direction of the column 5 Insert the glass plate into the mechanical stage Focusing drive and column 1 Unscrew the extension plate from the base using the Allen key provided 2 Attach your focusing drive column from below using the three hexagon head screws 3 Re attach the adapter plate to its original position using the three hexagon head screws ...

Page 43: ... will have to be mounted later The motorized mechanical stage is a sensi tive precision instrument During installation avoid subjecting the stage to impact or severe vibrations 1 Unpack the mechanical stage from the transport packaging and position it on the transmitted light base 2 Secure the motorized mechanical stage to the base using three M4 screws Leica IsoPro Motorized Mechanical Stage Asse...

Page 44: ...cal stage 4 Remove the two M4 screws and the sleeve from the mechanical stage 5 Remove the four shock absorbing cartons from the mechanical stage After removal keep all the transport anchors in the plastic bag provided for future transport Leica IsoPro Motorized Mechanical Stage Assembly Continued ...

Page 45: ... of the base without the stage mounted Disassembly is performed in reverse order of the following steps Mechanical stage and base Never move the sledge of the motor ized mechanical stage manually in the X direction as otherwise the mechanical system will be damaged 1 Place the mechanical stage onto the base 2 Pull the upper part of the mechanical stage carefully towards the user and fasten the low...

Page 46: ... connecting other instruments such as the Leica foot switch 10 447 398 or TL RCI transmitted light base 10 446 352 O O The 15 pin Sub D interface is intended for use with the Leica SmartMove controller 11 501 197 3 Plug a power cable into the socket provided and into a grounded power socket As soon as all instruments have been plugged into the control box and it has been plugged into the power sup...

Page 47: ...face to the optics carrier is on the column The terminals The connection to the PC and to other instru ments is made using the terminals on the rear side of the column 1 3 CTL2 terminals for auxiliary equipment such as the ring illuminator the base and other accessories from the Leica product range 2 Terminal for the power supply provided 3 USB terminal for the connection to the PC Cables Connecti...

Page 48: ...camera can be stowed in the cable duct Feeding the cables 1 Unscrew the three screws on the cable duct 2 Remove the cover of the cable duct 3 Place the cables in the cable duct and screw the cover on tightly Tip Estimate the length of the cable ends you will need before screwing on the cover For thick cables it is difficult to change the length retroactively Cables Cable Duct ...

Page 49: ...Controlbox for IsoPro motorized stage PSC SmartTouch PC USB Cross Stage Smart Move Power USB LED5000 RL LED5000 MCI Please use the Leica Application Suite to configure the functions of the system Alternative installation 115 230 V LED5000 SLI Handwheel Footswitch es ...

Page 50: ...traints TheLeicaLED5000MCIcannotbeusedtogether with the objective nosepiece Assembly 1 Hold the LED5000 MCI with one hand and tightly screw the retaining stirrups on both top holes on the drive housing 2 Connect the CAN bus cable provided to either of the two sockets The flat part of the plug must be facing downwards 3 Plug the other end of the cable into one of the three CTL2 sockets on the colum...

Page 51: ...remain the same For such purposes the Leica LED5000 MCI is directly fastened to the column Installation on the column 1 Pull the retaining stirrup out of the Leica LED5000 MCI 2 Screw the retaining stirrup into the column at the notch using the single screw If you tighten the screw the retaining stirrup is automatically moved into the correct position 3 Push the Leica LED5000 MCI onto the retainin...

Page 52: ... planapochromat 1 and planapochromat 0 63 objectives With all other objectives the working distance is too low for adequate illumination The ring illuminator cannot be used together with the objective nosepiece Assembly 1 Connect the Leica CAN bus cable provided to the ring illuminator 2 Place the Leica LED5000 RL against the objective from below push it up as far as it will go and screw it into p...

Page 53: ...ways for example if simultane ously using the Leica LED5000 MCI system illu minator In this case the ring illuminator cannot be connected directly to the Leica LED5000 MCI If you work with the manual focusing column without integrated electronics the Leica LED5000 RL must be supplied with power via an external power supply unit not included in the delivery package Moreover the illuminator cannot b...

Page 54: ...ed in accordance with EN 61010 2 101 2002 EN 61010 1 2001 IEC 1010 1 2001 Safety requirements for electrical equipment for measurement control and laboratory use The devices and accessories described in this operating manual have been tested for safety and potential hazards The responsible Leica affiliate or the main plant in Wetzlar must be consulted whenever the device is altered modified or use...

Page 55: ...ks etc If the heat absorbing filter is not inserted or is defec tive the service life of the light guide will be decreased Theinteriorofthelampinsertedintothe unit contains highly toxic substances At the end of its service life dispose of the lamp according to applicable regulations The manu facturer of the lamp provides corresponding information for example in the lamp s shipping documents Follow...

Page 56: ...ed Electrical safety For indoor use only Supply voltage 100 240 V 10 Frequency 50 60 Hz Power consumption Max 210 VA Fuses 5 20 2 5 A slow blowing breaking capacity H see page 115 Ambient temperature 0 40 C Relative humidity 10 90 Overvoltage category II Pollution degree 2 ...

Page 57: ...ing packaging materials The lamp used in the instrument becomes very hot during operation and has high internal pressure when hot Before changing the lamp it is mandatory to allow the instrument to cool off for at least 20 minutes The Leica EL6000 compact light source comes with a factory supplied lamp but the lamp is not installed when shipped This minimizes the danger of damage to the lamp durin...

Page 58: ...Leica EL6000 on its side so that the opening faces you This makes it easier to insert the lamp 5 Pull the pressure bolt back towards the front plate using the lever 6 Insert the lamp Ensure that the groove in the contact surface of the lamp matches up with the corresponding plug in the lamp mount ...

Page 59: ...nt release the lever 8 Connect the plug of the lamp with the coupling in the instrument Ensure that no wires are touching the reflector of the lamp 9 Check to make certain that a heat absorb ing filter has been inserted into the shaft in order to protect the connected light guide 10 Close the housing cover and insert the screws ...

Page 60: ...m the high energy light 1 Unscrew the positioning screw and remove the protective cover of the fluorescence lamp housing 2 Insert the adapter into the fluorescence lamp housing and retighten the locking screw 3 Unscrew the three red protective caps on the light guide 4 Insert the short end of the light guide into the lamp housing of the stereo microscope as far as it will go 5 Securely tighten the...

Page 61: ...ight guide with a light input of the type Storz long as otherwise damage to the instrument and danger to the user can result risk of being dazzled Before you open the shutter the light guide must be connected on both sides Otherwise the escaping light can cause injury to eyes and skin and damage to furni ture Never look into the light escaping from the light guide 7 Connect the Leica LA6000 to the...

Page 62: ...p it is mandatory to allow the instrument to cool off for at least 20 minutes 1 Pull the power plug out of the socket so that the EL6000 is disconnected from the power system 2 Unscrew both screws of the cover using a 3 mm allen key 3 Remove the housing cover 4 Carefully lay the Leica EL6000 on its side so that the opening faces you This makes it easier to insert the lamp 5 Pull the pressure bolt ...

Page 63: ...ble into a free CAN port of your microscope Use the reduction piece from 25 to 9 pins to connect the cable with the serial port of the light source Configuring the shutter On the front panel of the lightsource is a switch that allows switching between always open and remote Set the switch to remote to ensure the correct function of the cable Using the Shutter When switching on the microscope the s...

Page 64: ...Leica M Series User Manual Quick Start Guide 64 Quick Start Guide ...

Page 65: ...o get right to work Therefore your next step should be to study the Quick Start Guide which outlines the most important steps at a glance This manual will then familiarize you with the finer details of your micro scope The following pages contain important practical information that makes using it every day easier Take time to read it it s worth it The M series Quick Start Guide included with inst...

Page 66: ...an M Series Microscope 1 Eyepieces for spectacle wearers with dioptric correction and eyecups 2 Trinocular tube 3 Fluorescence housing 4 Filter Changer 5 Display 6 Motorized Focus 7 Objective Nosepiece 8 Leica SmartTouch 9 UV protection screen 1 2 3 4 5 7 9 8 6 ...

Page 67: ... this Not to worry after a little while it will become automatic Reference value The distance between eye and eyepiece measures approx 22 mm for 10 23B wide field eyepieces for persons wearing glasses Adjusting the interpupillary distance 1 Look into the eyepieces 2 Hold the eyepieces with both hands Push the eyepieces together or separate them until you see a circular image 3 Slowly approach the ...

Page 68: ...piece Using the Eyepieces If you do not wear glasses 1 Hold the eyepiece firmly and rotate the eyecups forwards counterclockwise 2 If an eyepiece is equipped with the inte grated dioptric correction turn the value to the 0 mark The eyepieces form the connection between the tube and the eye of the observer Simply push them into the tube and they are ready to use Each eyepiece offers a certain magni...

Page 69: ...nt of the objective O O The focusing drive can be operated either left or right handed Focusing O O The inner coarse adjustment is used for covering great distances O O The outer fine adjustment is used for fine focusing Coarse fine adjustment The sharpness is adjusted using the coarse fine adjustment The resolution of the coarse fine adjustment is 1 µm The coarse fine adjustment carries a load of...

Page 70: ...focus movement too loose or too tight Does the outfit tend to slide downwards The resistance can be adjusted individually depend ing on the equipment weight and personal preferences as follows 1 Grip the outer drive knobs with both hands and turn them towards each other until the desired resistance is reached during focusing ...

Page 71: ... integrated zoom The name indicates the zoom range covered O O Leica M165 16 5 1 O O Leica M205 20 5 1 The rotary knob for the zoom can be used either left or right handed Zooming 1 Look into the eyepieces 2 Focus on the specimen 3 Rotate the magnification changer until the desired magnification is configured ...

Page 72: ...results etc can be reproduced more accurately Enabling and disabling ratchet steps 1 Push the top button downwards to disable the ratchet steps 2 Push the bottom button upwards to enable the ratchet steps Magnifications and fields of view The formula on page 157 provides additional information about the magnifications and field of view diameters with consideration given to the position of the magn...

Page 73: ...parfocal work O O If you are using an eyepiece with dioptric correction the procedure differs from this description For more information refer to page 79 O O For the procedure for adjusting the parfo cality for the objective nosepiece refer to page 29 O O For the procedure for adjusting the parfo cality for the Leica FluoCombi III refer to page 33 Parfocality 1 Enlarge the view to the maximum leve...

Page 74: ...he depth of field The depth of field or focus depth is the area of a specimen that is brought into sharp focus Closing the iris diaphragm O O Close the iris diaphragm by turning the knob to the left The subject appears darker and the depth of field increases Opening the Iris Diaphragm O O Open the iris diaphragm by turning the knob to the right The subject now appears brighter but the depth of fie...

Page 75: ...Leica M Series User Manual Eyepieces 75 Eyepieces ...

Page 76: ...cro scope but also has a critical effect on the maximum magnification The magnification factor is between 10 and 40 The following eyepieces are available for the M series Magnification Dioptric correction Order number 10 5 diopter settings 10 450 630 16 5 diopter settings 10 450 631 25 5 diopter settings 10 450 632 40 5 diopter settings 10 450 633 ...

Page 77: ...yepieces is a potential transmission method for bacterial and viral infections of the eye The risk can be kept to a minimum by using individual eyepieces or detachable eyecups Eyecups can be ordered separately Please contact your Leica partner Separate eyecups are an effective way of preventing infections ...

Page 78: ...ring your glasses look through the eyepieces and focus on the specimen 3 Rotate both eyepieces to the maximum value of 5 4 Hold one eye closed and rotate the other eyepiece in the direction until the speci men appears sharp 5 Then open the other eye and correct the diopter settings until the image is uniformly sharp Note that when using dioptric correction the advantage of parfocality is lost thus...

Page 79: ...correct setting of the diopters and the parfocality The following adjustments only have to be carried out once by each user Preparations O O Move the lever of the video phototube to the observation position and open the diaphragm O O If you are using the microscope carrier AX set it to stereoscopic observation 7 Look into the eyepieces 8 Slowly rotate each eyepiece individually in the direction un...

Page 80: ... it using the clamping screw Use with the Leica AX carrier If possible measure with the micro scope carrier AX in vertical position The measurements are more accurate without the convergence angle in the stereoscopic image Use Leica graticules make length measurements and counting easier particularly for work stations that are not equipped with a digital camera and LAS software The Leica graticule...

Page 81: ...Leica M Series User Manual Photography Video 81 Photography Video ...

Page 82: ...eica Microsystems offers a variety of adapters that can be used together with the 50 and 100 trinocular tubes Leica DFC cameras However if you require absolute control over the camera and need the capability for measurement evaluation and more in addition to photography the digital Leica DFC cameras are exactly right for you Together with the Leica Application Suite they provide virtu ally limitle...

Page 83: ...d concentrates on the center of the field of vision Cameras from third party suppliers In addition to Leica DFC cameras with the stan dardized C mount interfaces you can connect third party cameras to the microscope using a T2 bayonet adapter Stereo 2016 V 1 Name MPixel Sensor Size Pixel Size Cooling Interface C Mount MC170 HD 5 CMOS c 1 2 3 2 35 µm No USB2 HD 0 4 0 5 MC190 HD 10 CMOS c 1 2 3 1 67...

Page 84: ...otube 50 enables you to simultaneously view and photograph a specimen The available light is divided as follows O O 50 is available for the two eyepieces O O 50 of the light is diverted to the video photo beam path Assembly Fasten the trinocular tube 50 to the optics carrier instead of the binocular observation tube refer to page 24 ...

Page 85: ...asten the trinocular tube 100 to the optics carrier instead of the binocular observation tube refer to page 24 Switchover O O Turn the controller on the right side of the tube into the horizontal position in order to guide all available light into the eyepieces You can now observe the specimen O O Turn the controller on the right side of the tube into the vertical position in order to guide all av...

Page 86: ...ces to best fit their posture The photo slider switches the right beam path from 100 of light to the eyepieces to 100 light to the camera port In photo position the left beam path is closed to prevent stray light entering the tube Assembly Before mounting the ErgoTube please remove the transport lock screw under the tube Other wise the photo slider cannot be used Trinocular Video Phototube 100 Erg...

Page 87: ...Leica M Series User Manual Microscope Carrier 87 Microscope Carrier ...

Page 88: ...ies objectives the parfo cality is maintained even when objectives are changed meaning that the specimen remains in sharp focus during the change Older Leica objectives can continue to be used but without parfocality during the objective change Technical constraints The objective nosepiece cannot be used together with the LED5000 MCI system illumination as the objective nosepiece can no longer be ...

Page 89: ...Leica M Series User Manual Objectives and Optical Accessories 89 Objectives and Optical Accessories ...

Page 90: ...atic objectives the finest structures are visible with high contrast The sophisticated apochromatic correction allows these objectives to attain the highest color brilliance and fidelity Achromatic objectives The 0 32 0 5 0 63 0 8 1 1 5 2 achromatic objectives offer countless variants for selecting the object field diameter magni fication ranges and working distances see page 157 Planachromatic ob...

Page 91: ...Leica M Series User Manual Bases 91 Bases ...

Page 92: ...sembly of focusing drives 2 Removable glass plate 3 Controller for light intensity 4 Adjustment for deflection mirror Rear side of the transmitted light base TL ST 1 Screws for changing the halogen lamp 2 Power connection socket 3 Power switch Extension plate of the transmitted light base TL ST 1 2 3 4 1 2 3 ...

Page 93: ...ed The mirror is kept in the correct position at all times and permits smooth changeover between bright field and opaque transmitted light Bright field Bright field is suitable for examining translu cent objects featuring contrasting structures The object is directly illuminated from below and is seen in its natural colors against a bright background O O Move the slider backwards until the desired...

Page 94: ...n lamps with your bare fingers this drastically reduces the service life of the lamp Changing bulbs 1 Unscrew the two screws on the heat sink and pull the heat sink out along with the lamp 2 Carefully pull out the lamp and mount by pulling them upwards 3 Disconnect the lamp from the mount 4 Insert the new lamp into the mount and reinsert the lamp holder Precautionary measures When inserting the la...

Page 95: ... of focusing drives 2 Standard stage 10 450 562 3 Button to toggle between bright field and dark field 1 2 3 ExtensionplateoftheTransmitted lightbaseTLBFDF Adapter at the focusing drive Button to toggle between bright field and dark field Connector for cold light sources light conductor active f 10mm end tube f 13mm ...

Page 96: ...ent objects featuring contrasting structures The object is directly illuminated from below and is seen in its natural colors against a bright background O O Turn the control as far as it will go towards BF bright field Dark field In dark field illumination a ring illuminator is used in such a way that the direct light does not reach the objective without a specimen Only the structure of semitransp...

Page 97: ...CI 2 Extension plate for focusing drives 3 Standard stage 10 450 562 4 Filter holder 5 Control of top and bottom flaps of the Rottermann Contrast 6 Button for mirror and horizontal movement of the mirror 7 Transmitted light base 1 2 3 4 5 6 7 1 3 4 5 6 2 1 Power switch 2 Power connection socket 3 USB socket type B 4 USB socket type A 5 2 CAN bus 6 Screws for changing the halogen lamp ...

Page 98: ...liver even an illumination at low magnifications The black rotary knob on the left side of the transmitted light base can be used to rotate the built in path folding mirror and move it forwards back The concave cutout on the handle indicates the concave side of the mirror allowing intuitive operation at any time without eye contact The angle of light incidence in the specimen plane changes dependi...

Page 99: ...o interrupt your work click the potentiom eter 2 O O Click again to switch on the illumination The electronics returns the color tempera ture to the previously configured settings 1 2 Using a USB mouse only TL RCI The Leica USB mouse controls the CCIC and dimming function of the TL RCI base Connect the mouse to the corresponding USB port of the base O O The scroll wheel of the mouse is occupied by...

Page 100: ...lly act as spatial relief type images in the positive relief contrast like hills in the inverted relief contrast like valleys Increased contrast without relief is attained if both diaphragms are set to 45 A gap like illuminated area is created By tilting the deflec tion mirror slightly you can move the gap over the entire field of view and quickly toggle between positive and negative relief images...

Page 101: ...ight Suitable for semitransparent opaque speci mens such as foraminifera and fish eggs Move the deflection mirror until the desired data are visible Single sided dark field Suitable for fixed specimens and fine structures The flatter the angle at which the light beams are deflected into the specimen plane the darker the substrate appears A dark field like transmitted light is created Outlines fine...

Page 102: ... contrast Suitable for semitransparent and transparent specimens The phase structures look like hills The effect can be strengthened or weakened by gently tilting the deflection mirror Negative relief contrast Suitable for semitransparent and transparent specimens These settings result in a negative relief contrast Phase structures look like valleys The effect can be strengthened or weakened by ge...

Page 103: ...ages The dynamic effect makes it easy to distinguish phase structures from amplitude structures Constraints The relief methods provide good results from mid zoom to high magnifications and with 1 1 6 and 2 objectives In the lower zoom half and with weaker specimens the object field may not be uniformly illuminated We recommend using the transmitted light base with 1 or higher objectives and not ob...

Page 104: ...ustomer request the filters are also available as one off items 1 Switch off the light source or click Leica TL RCI the button for the shutter 2 Take the empty filter from an available filter slot in the filter holder 3 Insert the desired filter 4 Switch the light source back on Daylight filter for Leica TL ST A daylight filter is also available for the LeicaTL ST transmitted light base Using Filt...

Page 105: ...al Bases 105 Leica IsoPro Non motorized Controls Operating the Leica IsoPro mechanical stage 1 To move the stage in the X direction rotate the outer knob 2 To move the stage in the Y direction rotate the inner control ring 1 2 ...

Page 106: ...stage 2 Housing with motorization 1 Sub D interface for Leica SmartMove 2 3 CTL2 interfaces 3 USB interface type B 4 Socket for grounded power cable 1 Quick control memory function 2 Fine control in X direction 3 Fine control in Y direction 1 2 1 2 3 4 IsoPro X Y Stage DCI module Leica PSC Controller 2 1 3 ...

Page 107: ...Leica M Series User Manual System Illumination 107 System Illumination ...

Page 108: ...the bottom end of the guide rods In this position you always have the same contrast with identical illumination This guar antees the reproducibility of an experiment Contact with the base If the optics carriers are accidentally lowered too far contact between the base and MCI may result A safety mechanism in the linkage ensures that in this case the MCI is automatically pushed upwards to prevent t...

Page 109: ...and keys to toggle between the illumination scenarios shown below Maximum brightness For temperature reasons it is not possible to switch on all nine LEDs simultaneously There fore to provide bright overall illumination the top two rows are activated The bottom row primarily provides contrast The Leica LED5000 MCI can also be controlled by the LAS Leica Application Suite software Leica LED5000 MCI...

Page 110: ...utomatically toggle between them For additional information refer to the LAS online help Using the keyboard O O Use the key to switch the illumination on or off O O Use the and keys to adjust the brightness in 10 increments Touch either of the two buttons to adjust the intensity in small increments Hold a key to change the intensity more quickly The illumination scenarios Use the and keys to toggl...

Page 111: ...ched This prevents the user from being harmed by the intense light beam generated by the instrument The Leica EL6000 compact light source has a built in shutter It can be operated at the instru ment itself by means of a pushbutton or via a remote control input The shutter is open when the open LED is illuminated The brightness can be varied in 5 increments 0 100 For this purpose a mechanical dimmi...

Page 112: ...light source or the microscope adapter make sure not to kink or damage the light guide Your Leica consultant will be glad to provide additional information about suitable light guides Identification of the instrument The nameplate of the instrument is on its bottom It includes the necessary information for identifying the instrument TheLeicaEL6000compactlightsourcecomplies with Council Directive 9...

Page 113: ...placement so that it always displays the current accurate lamp life Discontinue using the built in lamp when it reaches the end of the life specified by the manufacturer Shutter control O O In the open position the shutter of the Leica EL 6000 is open regardless of the signal from the remote control O O If the shutter control is in the remote posi tion the shutter is controlled by the remote contr...

Page 114: ...ly see page 115 Power switch is illuminated after being switched on instrument shows no function Housing cover loose or removed Attach housing cover securely Lamp worn out Replace lamp Target system receives too little light or none at all Shutter closed or intensity switch at left limit stop Open shutter or rotate intensity switch towards the right Light guide not inserted into light guide output...

Page 115: ...g Spare parts and accessories The only parts of the Leica EL6000 compact light source that may be replaced by the user are the instrument fuses and the lamp f f The replacement of the lamp is described on page 62 Replacing the instrument fuse 1 Disconnect the Leica EL6000 from the power supply 2 Open the drawer with the instrument fuses located close to the power supply input 3 Replace the instrum...

Page 116: ...Leica M Series User Manual Fluorescence 116 Fluorescence ...

Page 117: ...ce These filter holders have two empty openings for the observation beam paths The third open ing to the illumination beam path is closed If you are using fewer than four filter holders with fluorescence filters always insert the included simple filter holders with closed illumination beam path into the avail able positions Otherwise there is a risk of danger to the eyes from direct UV radiation f...

Page 118: ...ensity of the excitation light can be reduced in five fixed steps The light can be changed from 100 to 55 30 and 17 down to 10 The advantage of this procedure is its absolute reproducibility and fast switching between the intensity levels A separate FIM level can be stored for every fluorescence filter Different intensities of fluorescence can thus be cali brated to one another ...

Page 119: ...series stereo microscopes your first choice The patented TripleBeam third beam path for fluorescence illumination and the patented FLUOIII filter system generate highest quality fluorescence images The very bright high pressure mercury burner and specially selected filter sets enable you to distinguish the finest structures and enhance the quantity of infor mation for incident light fluorescence T...

Page 120: ...nm 460 LP V Blue 470 40 nm 450 490 nm 515 LP B Green 546 10 nm 541 551 nm 590 LP G CFP 436 20 nm 426 446 nm 480 40 nm 460 500 nm CFP YFP 510 20 nm 500 520 nm 560 40 nm 540 580 nm YFP Texas Red 560 40 nm 540 580 nm 610 LP TXR DsRED 545 30 nm 510 560 nm 620 60 nm 590 650 nm DSR CY5 620 60 nm 590 650 nm 700 75 nm 663 737 nm CY5 CY3 555 60 nm 530 580 nm 610 75 nm 573 648 nm CY3 The rapid filter change...

Page 121: ...ury light for longer than 15 seconds Safety Notes O O If you want to block the mercury light for longer than that activate the UV shutter O O Use the filter if you want to change to trans mitted light or incident light observation without fluorescence O O Always place a simple filter holder into the empty filter changer positions in order to protect yourself from UV radiation and protect the syste...

Page 122: ...hese must be removed before inserting a filter 1 Grasp the recess of the dummy in the filter changer and pull it out Inserting the filter 1 Place the filter into the filter changer Ensure that the contour of the filter holder matches the contour of the microscope 2 Rotate the filter changer by hand until it latches into place If the filter holder has not been inserted correctly the filter changer ...

Page 123: ...e system only Observation without Fluorescence The FLUOIII filter system includes a UV shutter that can be used to close the illumination beam path This feature protects the mercury lamp which should not be switched on and off too frequently If for instance you need to inter rupt your work you do not have to switch off the high pressure mercury burner to preserve sensitive specimens from being bur...

Page 124: ...ouble iris diaphragm regulates the depth of field Note that O O The greater the depth of field the darker the image becomes O O In the same way the exposure time becomes longer for photographs O O The resolution decreases Adjusting the double iris diaphragm using the knurled ring ...

Page 125: ...on the supply unit until you feel confi dent using the Leica stereo microscope Follow all safety instructions when handling mercury lamps and the lamp housing 1 Switch on the supply unit see User Manual M2 216 1 2 Wait 2 3 minutes 3 Adjust the arc lamp in accordance with User Manual M2 216 1 For observation with fluorescence light always position the protection screen correctly to protect the obse...

Page 126: ...Leica M Series User Manual Accessories 126 Accessories ...

Page 127: ...oot switches can be configured with a microscope system They can be programmed in LAS or LAS X to control focus filter wheel illumination and individual memory positions Leica Hand Wheel and Foot Switch The hand wheel is designed to control the motor focus ...

Page 128: ...or handling motorized Leica instruments Controls The functions of the individual controls can be configured both directly on the Leica Smart Move and using the Leica Application Suite For a detailed description of the functions and settings please refer to the Leica SmartMove manual or the Leica Application Suite help file Leica SmartMove ...

Page 129: ...able rotary knobs and function buttons Controls The functions of the individual knobs and screen elements can be configured both directly on the Leica SmartTouch and using the Leica Application Suite The standard configuration when shipped from the factory is as follows Leica SmartTouch O O Top part of the knob zoom O O Upper part of the knob focus O O Buttons left filter right filter shutter For ...

Page 130: ...ng over the specimen quickly O O Move the joystick in any desired direc tion including diagonally to control the mechanical stage quickly Fine control of the mechanical stage The motorized Leica IsoPro mechanical stage offers an accuracy of up to 0 25 µm To move to a position with maximum accuracy use the left and right knobs on the joystick Control by LAS The instructions for controlling the Leic...

Page 131: ...Leica M Series User Manual Dimensional Drawings 131 Dimensional Drawings ...

Page 132: ... Dimensional Drawings 132 Leica M165 FC Leica M165 FC with transmitted light stand and focusing column dimensions in mm 385 154 95 154 292 217 385 154 340 390 440 95 154 116 431 547 292 217 385 154 340 390 440 95 154 116 431 547 ...

Page 133: ... Leica M165 FC with transmitted light stand TL RCI manual cross stage Leica IsoPro and manual focus 497 292 217 187 154 max 370 95 116 431 547 497 292 217 187 154 max 370 X 75 mm 370 95 300 249 Y 50 mm 116 431 547 497 187 154 max 370 X 75 mm 370 95 300 249 Y 50 mm 116 431 547 ...

Page 134: ...l Dimensional Drawings 134 Leica M165 FC Continued Leica M165 FC with transmitted light stand TL RCI and manual focus 85 154 356 154 292 217 106 431 340 430 85 154 356 154 292 217 106 431 537 340 430 85 154 356 154 106 431 537 ...

Page 135: ...ngs 135 Leica M205 FA Leica M205 FA with transmitted light stand TL BFDF trinocular ErgoTube DFC camera and motorized focus dimensions in mm 370 154 90 154 292 116 431 547 370 154 340 390 90 154 292 116 431 547 370 154 340 390 90 154 116 431 547 ...

Page 136: ...awings 136 Leica M205 FA Continued Leica M205 FA with transmitted light stand TL RCI trinocular ErgoTube DFC camera and motorized focus 385 154 5 154 116 431 292 385 154 340 390 440 95 154 116 431 547 292 385 154 340 390 440 95 154 116 431 547 ...

Page 137: ...ica M205 FA with transmitted light stand TL RCI IsoPro mechanical stage trinocular ErgoTube DFC camera and motorized focus 497 187 154 95 292 116 431 497 187 154 max 410 X 75 mm 370 95 300 249 Y 50 mm 292 116 431 547 497 187 154 max 410 X 75 mm 370 95 300 249 Y 50 mm 116 431 547 ...

Page 138: ...onal Drawings 138 Leica M205 FCA Leica M205 FCA with transmitted light base and focusing column dimensions in mm 385 154 95 154 292 217 385 154 340 390 440 95 154 116 431 547 292 217 LEICA M205 FCA 385 154 340 390 440 95 154 116 431 547 ...

Page 139: ...5 FCA with transmitted light stand TL RCI Leica IsoPro manual mechanical stage and manual focus 497 292 217 187 154 max 370 95 116 431 547 497 292 217 187 154 max 370 X 75 mm 370 95 300 249 Y 50 mm 116 431 547 LEICA M205 FCA 497 187 154 max 370 X 75 mm 370 95 300 249 Y 50 mm 116 431 547 ...

Page 140: ...ional Drawings 140 Leica M205 FCA Continued Leica M205 FCA with transmitted light stand TL RCI and manual focus 85 154 356 154 292 217 106 431 340 430 85 154 356 154 292 217 106 431 537 LEICA M205 FCA 340 430 85 154 356 154 106 431 537 ...

Page 141: ... User Manual Dimensional Drawings 141 Leica LED5000 MCI 132 275 3 157 83 5 112 45 112 90 45 36 22 132 275 3 157 83 5 112 45 112 90 45 36 22 132 275 3 157 83 5 112 45 112 90 45 36 22 Leica LED5000 MCI dimensions in mm ...

Page 142: ...Leica M Series User Manual Dimensional Drawings 142 Leica LED5000 RL 132 132 160 37 160 37 58 mm Leica LED5000 RL dimensions in mm ...

Page 143: ...Leica M Series User Manual Dimensional Drawings 143 Leica MATS TL 190 140 220 4 11 134 98 170 10 447 164 10 447 275 ...

Page 144: ...Leica M Series User Manual Specifications 144 Specifications ...

Page 145: ...3 lp mm 61 5mm planapochromat 31 5mm 1 92mm 7 8 160 max 525 lp mm 61 5 mm planapochromat 29 5 mm 1 44 mm 7 8 160 max 525 lp mm 61 5 mm planapochromat 29 5 mm 1 44 mm Maximum values based on optics combination Magnification Resolution Visible structural width Numerical aperture Object field 960 906 lp mm 551 nm 0 302 63 mm 1 280 1 050 lp mm 476 nm 0 35 59 mm 1 280 1 050 lp mm 476 nm 0 35 59 mm Work...

Page 146: ...sepiece Zoom iris diaphragm objective nosepiece FIM filter Zoom iris diaphragm objective nosepiece filter Engageable zoom notches 13 for repetitive tasks 14 for repetitive tasks 14 for repetitive tasks Double iris diaphragm for depth of field control Built in and coded Built in and motorized Built in and coded FIM Fluorescence Intensity Control Built in and motorized Accessories Standard objective...

Page 147: ...pochromat Interpupillary distance 50 mm 105 mm Wide field eyepieces for persons wearing glasses 10 16 25 40 with replaceable eyecups click stop Manual coarse fine focus Focusing range 130mm or 330 mm adjustable ease of movement Focusing range 130mm adjustable ease of movement Focusing range 130mm adjustable ease of movement Motorized focusing drive Motorized focusing drive with 431mm profile colum...

Page 148: ...5000 RL fluorescence oblique coaxial cold light sources Misc accessories Photography video Leica DFC digital image capture system various camera variants Various adapters for commercially available CCD cameras Image archiving image processing Leica AF6000 Special software for all fluorescence tasks Leica Application Suite LAS consisting of basic program and various auxiliary modules Measurement gr...

Page 149: ...1326 Class B Immunity to interference EN 61326 Appendix A Voltage fluctuations flicker EN 61000 3 3 Harmonic currents EN 61000 3 2 The instrument fulfills the requirements of EC Directive 89 336 EEC and the EMC law of September 18 1998 Electrical safety in accordance with EN 61010 1 2001 Pollution degree 2 Overvoltage category II Power input Design Switched socket IEC320 C13 EN 60320 C13 Power sup...

Page 150: ...hutter closed Connection between pin 2 and 4 Current Approx 1 2 mA Shutter frequency Max 10 Hz Protective functions Safety circuit If the cover is open all components in the instru ment except for the power input and power plug are disconnected from the power supply voltage Overheating protection In the event of overheating the lamp supply is shut off After cooling off the lamp power supply in the...

Page 151: ... ambient temperature 10 40 C Connections Power plug power switch Weight 7 4 kg Illumination types Bright field Yes Dark field Yes single sided Oblique light No Relief Contrast System RC No CCIC Constant Color Intensity Control No Internal shutter lamp control No Integrated filter holder Yes Coated optics for increasing the color temperature Yes Matching of high num aperture No Remote control optio...

Page 152: ...umination types Bright field Yes Dark field Yes Oblique light No Relief Contrast System RC No CCIC Constant Color Intensity Control No Internal shutter lamp control Yes Integrated filter holder No Coated optics for increasing the color temperature No Matching of high num aperture Yes Remote control options Yes AntiShock Pads Yes Dimensions W H D 340 390 90 mm With cold light source Leica CLS150 LS...

Page 153: ...f 13mm 1 USB type A 1 USB type B 2 CAN BUS Weight 6 0 kg 7 2 kg Illumination types Bright field dark field Yes yes Yes yes Oblique light Relief Contrast System RC Yes yes Yes yes CCIC Constant Color Intensity Control No Yes Internal shutter lamp control Yes Yes Integrated filter holder Yes Yes Coated optics for increasing the color temperature Yes Yes Matching of high num aperture Yes Yes Remote c...

Page 154: ... Yes Remote control options Yes Integrated filter holder Yes Matching of high num aperture Yes Anti Shock Pads Yes Size W H D in mm 412 341 46 Accessories IsoPro cross stage support Integrated heating stage optional Yes Integrated slide adapter optional Yes ErgoRest optional Leica TL5000 Ergo Power supply Input 120 240VAC 47 63Hz 3 2A Output 33 VDC 3 93 A 130 W max Connections CAN 3 USB 1 Power Su...

Page 155: ...r resolution X Y 0 25µm Repeatability X Y 2µm Positioning accuracy X Y 20 µm over the entire positioning range Hysteresis X Y 30µm Motor Direct current DC motor with encoder Backlash Slack free Adapter All adapters for TL bases Acceleration S shaped acceleration Computer connection USB Control options Leica PSC Leica Smart Move Leica SmartTouch Leica LAS via PC Power supply Input 100 240VAC 50 60H...

Page 156: ...Leica M Series User Manual Appendix 156 Appendix ...

Page 157: ... if the planachromatic and planapochromatic objectives of the MZ125 MZ16 are used on the MS5 MZ6 MZ75 or MZ95 NFOV Field number of the eyepiece Field numbers are printed on the eyepieces 10 21 16 14 25 9 5 40 6 Example MO 1 objective ME 25 9 5 eyepiece z Zoom position 4 q Coaxial reflected light 1 5 tube factor r Factor 1 25 Calculation example Magnification in the binocular tube MTOT VIS MO ME z ...

Page 158: ...resulting from careless or improper handling Contact address However if your instrument should no longer function properly contact your technician your Leica representative or Leica Microsystems Switzerland AG CH 9435 Heerbrugg E mail contact stereo service leica microsystems com Care O O Protect your microscope from moisture fumes and acids and from alkaline caustic and corrosive materials and ke...

Page 159: ...nents Some components are made of polymer or are polymer coated They are therefore pleasant and convenient to handle The use of unsuit able cleaning agents and techniques can damage polymers Permitted measures O O Clean the microscope or parts of it using warm soapy water then wipe using distilled water O O For stubborn dirt you can also use ethanol industrial alcohol or isopropanol When doing so ...

Page 160: ...bject to modifications LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH CONNECT WITH US www leica microsystems com Leica Microsystems Schweiz AG Max Schmidheiny Strasse 201 9435 Heerbrugg Switzerland T 41 71 726 34 34 F 41 71 726 34 44 ...

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