Leica DMLS, Instructions Manual

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35
The double engraving
λ
and
λ
/4 on the con-
denser disc is without significance here (the
disc can be optionally equipped with light rings,
whole- and quarter-wave compensators for
polarization (9.6) or with the 2.5x lens (9.7).
Make sure to open the aperture diaphragm
(23.7) (= pos.
PH ).
Auxiliary telescope
Insert an auxiliary telescope* (25.1) into the
observation tube in place of an eyepiece.
Slightly loosen the clamp ring (25.3) and focus
the annular structures by adjusting the eyelens.
Retighten the clamp ring. Does not apply for
configuration with light ring slides (8.7).
Centering the light rings
Condenser UCL/UCLP (7.1):
Push in the two centering keys (7.5; 8.3) at the
back of the condenser (23.2; 9.3) and rotate until
the dark ring (PH = phase ring in the objective)
coincides with the slightly narrower bright ring
(LR = light ring in condenser),
→
Fig. 26a – c.
Watch the quality of the phase contrast image. If
using the auxiliary telescope, watch the image
with one eye through the eyepiece. Then repeat
the centration process for the other objective
light ring combinations.
CL/PH condenser (7.4; 7.7): with light ring slides
(8.7). Centration is not necessary.
Fig. 25
1
Auxiliary telescope, 2 Adjustable eyelens, 3 Clamp ring for
fixing the focus position, 4 Immersion oil, 5 Light trap for
fluorescence (intrruption of transmitted light,
→
31.11)
Fig. 26 Centration process for phase contrast, observed with
an auxiliary telescope
a Condenser in brightfield position (BF), PH = Light ring in
objective, b Condenser in PH position, light ring LR not
centered, c Light ring and phase ring centered
4 1
2
3
5
a b c
PH LR PH LR+PH