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74

Instructions for Use, V 1.9 RevJ – 08/2017

5.6  The online help

The Leica ASP300 S has an online help that can be started from every main 

window. It contains a reference to the Instruction for Use.
The online help can be accessed from any screen by pressing the 

HELP

 

button.

5. Operation

Fig. 78

Summary of Contents for ASP300 S

Page 1: ...eica ASP300S Advanced Smart Processor Vacuum Tissue Processor Leica ASP300S English Order No 14 0476 80101 RevJ Always keep this manual with the instrument Read carefully before working with the instrument V 1 9 RevJ 08 2017 ...

Page 2: ......

Page 3: ...etails of the present Instructions for Use are not to be considered as warranted characteristics of our products These are determined only by the contract provisions agreed between ourselves and our customers Leica reserves the right to change technical specifications as well as manufacturing process es without prior notice Only in this way is it pos sible to continuously improve the technology an...

Page 4: ... Technical Data 24 3 5 Compatible reagents 26 3 6 Recommended reagent handling 27 3 6 1 Cycle for changing reagents 28 4 Initial Operation 29 4 1 Installation site requirements 29 4 1 1 Moving the instrument 29 4 2 Electrical connection 30 4 2 1 Connecting the power supply 30 4 2 2 Connecting an uninterruptible power supply UPS 31 4 3 Installing accessories 32 4 4 Making the data connections 34 4 ...

Page 5: ...5 3 2 Starting a program 69 5 3 3 Opening the retort 70 5 3 4 Retort emergency release 70 5 4 Reagent status 72 5 5 System monitor 73 5 6 The online help 74 6 Troubleshooting 75 6 1 General 75 6 2 Power failure 75 6 3 Troubleshooting 76 6 4 Typical fill or drain problems 77 7 Cleaning and maintenance 78 7 1 Clean programs 78 7 1 1 Retort cleaning programs 78 7 1 2 Paraffin cleaning 80 7 1 3 Smart ...

Page 6: ...ription Unit wired for 100 120 V alternating current label attached at the rear of the instrument close to the input socket of the power supply see fig 4 41 Symbol Title of symbol Caution 230 240 V Description Unit wired for 230 240 V alternating current label attached at the rear of the instrument close to the input socket of the power supply see fig 4 41 Symbol Title of symbol Caution Descriptio...

Page 7: ...rface Description Instrument surfaces which become hot during operation are marked with this symbol Avoid direct contact to prevent risk of burning Symbol Title of symbol Manufacturer Description Indicates the manufacturer of the medical product Symbol Title of symbol Date of Manufacture Description Indicates the date when the medical device was manufactured Symbol Title of symbol CE Compliance De...

Page 8: ...L the Canadian Standards Association CSA the National Sanitation Foundation International NSF and others Symbol Title of symbol In vitro diagnostic medical device Description Indicatesamedicaldevicethatisintendedtobeused as an in vitro diagnostic medical device Symbol Title of symbol Alternating current Symbol Title of symbol Stack limit Description The largest number of identical packages allowed...

Page 9: ... the Environment friendly Use Period of the product in years The symbol is used if a substance restricted in China is used in excess of the maximum permitted limit Symbol Title of symbol WEEE Symbol Description The WEEE symbol indicating separate collection for WEEE Waste of electrical and electronic equipment consists of the crossed out wheeled bin 7 ElektroG Symbol Title of symbol Article number...

Page 10: ...ng agents are marked with this symbol Symbol Title of symbol IPPC symbol Description The IPPC symbol includes IPPC symbol Country code to ISO 3166 e g DE for Germany Regional identifier e g HE for Hesse Registration number unique number beginning with 49 Treatment method e g HT heat treatment Symbol Title of symbol Fragile handle with care Description Indicates a medical device that can be broken ...

Page 11: ...the medical device can be safely exposed Symbol Title of symbol Humidity limitation for transport and storage Description Indicates the range of humidity for transport and storage to which the medical device can be safely exposed Appearance Title of symbol Tilt indicator Functional description Indicator to monitor whether the shipment has been transported and stored in upright position according t...

Page 12: ...ated to operate this instrument must read these Instructions for Use carefully and must be familiar with all technical features of the instrument before attempting to operate it 1 2 Qualification of personnel 1 3 Intended use of instrument Any other use of the instrument is considered improper Failure to adhere to these instructions may result in an accident personal injury damage to the instrumen...

Page 13: ...trument is working with an embedded Windows 7 operating system Terms of the so called End User License Agreement EULA apply For details please refer to the language CD where you can find the entire content of this agreement 1 4 Instrument type 1 5 Microsoft software license terms Fig 1 Fig 1 is provided as an example only and shows valid nameplates for this instrument with the necessary informatio...

Page 14: ...Be sure to read these notes even if you are already familiar with the operation and use of other Leica products The protective devices located on the instrument and the accessories must not be removed or modified Only qualified service personnel authorized by Leica may repair the instrument and access its internal components Residual risks The instrument has been designed and constructed with the ...

Page 15: ...ure to comply with the following instruc tions and warnings Warnings Markings on the instrument itself Markings on the instrument showing the warning triangle indicate that the correct operat ing instructions as defined in these Instructions for Use must be followed when operating or replacing the item marked Failure to adhere to these instructions may result in an accident personal injury damage ...

Page 16: ...t interfere with the grounding function by using an extension cord without a ground wire Make sure to observe the voltage settings The set voltage CANNOT be changed by the user Severe damage may occur if the instrument is connected to a power supply voltage other than that to which it was originally set The installation location must be well ventilated there should be no ignition sources there of ...

Page 17: ...man tissue is being processed the instrument is connected to a local and or remote alarm system see Chap 4 6 to prevent the possibility of the specimen being lost in case of a malfunction Before opening the retort when an infiltration process is in progress always press the PAUSE button so that the retort is ventilated or vented The paraffin drain hose and the hose for remote filling draining are ...

Page 18: ...erve the applicable local regulations and the waste disposal regulations of the company institution in which the instrument is being operated Do not clean reagent containers bottles in a dishwasher the containers are NOT dishwash er proof Switch off the instrument each time before servicing and pull out the power plug Do not use inflammable cleaning agents to clean hot surfaces Do not clean the in...

Page 19: ...t vents all excess air pump output to atmosphere Overcurrent protection Overcurrent conditions are protected against by both the main fuse and the separate heating power fuses Overheating protection An error is indicated and all heating is stopped by the microprocessor control if the instrument detects any of the following conditions Abnormally high temperature 75 C Contradictory results of the te...

Page 20: ...20 Instructions for Use V 1 9 RevJ 08 2017 3 1 Overview instrument components 3 Instrument Components and Specifications 2 3 4 5 11 6 7 12 13 14 15 16 17 18 19 20 10 9 8 1 Fig 2 21 ...

Page 21: ...n spout 16 Remote drain connection 17 Retort 18 Wax baths 19 Cassette basket 20 Lid for cassette basket 21 Baffle plate 3 Instrument Components and Specifications Instrument Components and Accessories Three wax baths and the retort comprise the infiltration module The touchscreen and the electronic components are there The cassettes to be processed are stored in three baskets 19 each of which can ...

Page 22: ...s the reagents in the correct sequence organized in ascending order of cleanliness Liquid movement wave motion during the process for better and continuous mixture of the reagents System for non contact filling draining of reagents drains and fills the reagent container using a hose connected to the infiltration module without the operator being exposed to reagents in the process Non contact wax b...

Page 23: ...Filling draining hose assembly 14 0476 34716 1 Paraffin drain hose 14 0476 34721 1 Paraffin scraper plastic 14 0476 35923 1 Lubricant for valves and O rings Molykote 111 100 gr 14 0336 35460 1 Connecting cable power supply 14 0411 34604 1 Maintenance kit 2 spare covers 9 O rings 14 0476 35921 1 Baffle plate 14 0476 34770 1 Single head wrench size 27 14 0330 50891 1 Allen key size 3 0 14 0222 04138...

Page 24: ...pty weight unpacked approx 160 kg Weight packed 220 kg Operating temperature range 15 C to 35 C Relative humidity 10 to 80 non condensing IEC 61010 1 classification Protection class 1 Pollution degree 2 Overvoltage installation category II 800 V impulse 120 V systems 1500 V impulse 240 V systems Altitude 2000 m maximum Local remote alarm relay 30 VDC maximum 2 A 2 terminals Each with isolated swit...

Page 25: ...Drain time approx 80 120 140 sec selectable Impregnation vacuum 70 kPa g Infiltration pressure 35 kPa g Filling vacuum 70 kPa g Draining pressure 35 kPa g General Reagent container 10 Cleaning solution bottles 3 Maximum bottle volume 5 0 l Pretest check ON OFF Fluid level sensor ON OFF Recirculation pump in out ON OFF a Time before 1st cycle 16 min b Time between cycles 20 min System setup Passwor...

Page 26: ... clean programs Paraffin cleaning program 10 reagent containers 3 paraffin containers 3 cleaning solution bottles 1 condensate container Reagent temperature selectable from 35 C to 55 C or room temperature Paraffin temperature selectable from 40 C to 65 C Choice of three retort drain rates of 80 120 and 140 seconds Up to 100 reagent names in memory Use of the ASP300S is permitted with the reagents...

Page 27: ...and shorten instrument life If you choose to work with such fixatives it is essential to perform a clean cycle which contains multiple water rinses each time after use to minimize damage In addition we recommend frequent and regular preventive maintenance by the Leica Technical Service 3 5 Compatible reagents continued 3 6 Recommended reagent handling The reagents used should be replaced after 120...

Page 28: ...6 cleaning cycles Run the paraffin cleaning program once a week Run the paraffin cleaning program twice a week Run paraffin clean ing program daily Replace the paraf fin after 6 cleaning cycles Paraffin 2 Run the paraffin cleaning program twice a week Run paraffin clean ing program daily Replace the paraf fin after 6 cleaning cycles Run the paraffin cleaning program once a week Run the paraffin cl...

Page 29: ...flammable and noxious The installation site for the Leica ASP300S must be well ventilated and there must be no ignition sources of any kind in the area Never operate the instrument in rooms with an explosion hazard A connection to an external exhaust system a technical room ventilation system and an integrated exhaust system with an active carbon filter reduce the concentration of solvent vapor in...

Page 30: ...to the in strument refer also to Chap 2 2 Warn ings Transport and installation 41 4 2 1 Connecting the power supply 43 37 38 40 42 Left Fig Electrical ports on the rear panel of the instrument Fig 4 Right Fig Correct connection of the cables Once the instrument has been switched on the main switch ON OFF 5 should always remain in the ON position Connect the power cable 37 to the input socket 41 of...

Page 31: ...2 Connecting an uninterruptible power supply UPS 38 38 40 37 38a Fig 4a is provided as an example only and shows the correct connection of the ASP300S to an uninterruptible power supply UPS Fig 4a An uninterruptible power supply UPS protects machines and instruments against malfunctions in the power supply Leica recommends using an active tracking UPS with an output power of 1000 1500 VA to protec...

Page 32: ...ling accessories 18 29 Remote fill drain hose Connect the supplied remote fill drain hose to the remote drain connection on the front of the instrument refer Fig 6 Important Wheninsertingthehoseintothedrainopening Fig 5 6 18 the connecting device 28 of the hose must engage with a clearly audible click Fig 6 28 18 If it is possible to set up the bulk con tainer for filling and draining in the immed...

Page 33: ...g 7 and insert it into the retort Important The magnetic stirrer must be inserted such that the outer curvature 32 of the two stir rer blades 31 faces downwards Fig 7 Do not insert them the other way around as then there will be no effective stirring 32 31 30 4 3 Installing accessories continued ...

Page 34: ...ial Operation Fig 8 For data transfer and storage the instrument console is equipped with an USB port 11 An USB memory stick is part of the standard deliv ery For information on alarm connections see Chap 3 1 6 10 9 11 4 4 Making the data connections ...

Page 35: ...moved from the basket Then insert a suitable tool screwdriver 5 Fig 11 and pry the clip out from the inside 5 1 2b 2a 1 3 4 4 4 Fig 11 Insert the left lug 2a of the anti reflection clip 1 into the seventh hole from the left of the lower oval openings Fig 9 Snap the right lug 2b Fig 10 into the seventh hole from the right with gentle pressure 4 Initial Operation The anti reflection clips are intend...

Page 36: ... alarm system optional If required connect the local or remote alarm system to the appropriate sockets 34 Ø 6 3 mm using the stereo jack provided 9 10 in Fig 8 Local alarm socket 9 Remote alarm socket 10 Each alarm is connected to the plug 34 as follows see Fig 12 Shared terminal Tip 36 connection inside Normally Open Contact Connection First neck 35 connection outside Normally Closed Contact Conn...

Page 37: ...uchscreen to restore the screen After having been restored the on screen func tions will be nonoperational for a few seconds to avoid accidental activation of any keys Fig 14 Fig 13 4 Initial Operation The instrument MUST be connected to a grounded power socket For additional electrical fuse protection we recommend connecting the Leica ASP300S to a socket with a residual current circuit breaker Co...

Page 38: ...LATION menu 3 Aborts the current program 4 Aborts the current program and deletes the current allocation of reagents to bottles and retort 5 Deletes all reagents and resets programs and instrument status all lists will be empty 6 Saves the current instrument status 7 Displays the results log 8 Restarts the instrument To exit this menu the device must be restarted press RESTART APPLICATION To reini...

Page 39: ...at it performs is not applicable at the moment Disabled Enabled Disabled icons have a thinner border than active ones as shown here If a disabled button is pressed a help text will be displayed explaining why this particular button is disabled Button symbols Pressing a button on the Leica ASP300 S activates the corresponding in strument function All buttons have a uniform design for easy identific...

Page 40: ...esired reagents and the threshold values for the CHANGE REAGENT warning Assign a reagent to each station bottle Fill the reagent containers Remote filling SMART SCREENS Manual filling REAGENT STATUS Create the required programs Both infiltration and cleaning programs can be copied and modified Assign your favorite programs to the Favorites menu and if desired assign the end time and a symbol for t...

Page 41: ...owing message will be displayed After completion of the shutdown procedure a black screen will be dis played Then you can switch off the instrument with the ON STOP switch on the right of the instrument and with the ON OFF switch on the rear panel of the instrument item 42 in Fig 4 4 Initial Operation Notice The ASP300S is allowed to be switched off completely in this way only Otherwise severe dam...

Page 42: ...s The SYSTEM SETUP is divided into five areas Program options Display Buzzer Date Time Security Instrument Fig 23 5 1 1 System setup In the MORE MENU FUNCTIONS press SYSTEM SETUP Fig 21 From the start screen press MENU to switch to the MENU FUNCTIONS window and press the MORE button there Fig 22 ...

Page 43: ...Sequential otherwise the order of baths will be managed by the RMS PROMPT FOR NUM BLOCKS Enabled or Disabled Enabled When starting a program the actual number of blocks must be entered This is recommended with the RMS being enabled Disabled Number can be entered but does not have to be DATE TIME Be sure to verify that the date and time entered do actually correspond to the local time date as this ...

Page 44: ...affin to ensure that a minimum loss of temperature occurs when filling the retort DISPLAY BUZZER The number next to DISPLAY OFF indicates the remaining time in min until the screen saver after the last user action is activated The BUZZER VOLUME can be set between 1 and 10 Paraffin temperature and run options screen Fig 28 The RUN OPTIONS displays the options with which a program starts The options...

Page 45: ...ack border Supervisor access level Supervisors may perform all Operator functions and additionally create programs and perform the initial operation functions In supervisor mode the SMART SCREEN menu displays a status bar with information about the retort and the wax baths see Fig 53 To access Supervisor level press SUPERVISOR enter the required pass word and confirm After you enter the password t...

Page 46: ... entered Disabled All functions are accessible as long as the lock mode is deactivated Entry keyboard The keyboard is displayed any time text needs to be entered The keyboard headline 1 tells you what kind of text to enter 30 characters can be entered into each entry field though sometimes not all characters entered can be displayed Fig 31 Important keys Shift To shift to upper case symbols AltGr ...

Page 47: ...e key board Enter a name 20 characters maximum for the instrument The instrument name is also displayed on the FAVORITES screen Select a language Press the LANGUAGE key the SELECT THE LANGUAGE selection menu appears Select the desired language there and press OK Fig 32 A message box will prompt you to restart the instrument in order to display the user interface in the selected language Press YES ...

Page 48: ...r field and press OK This parameter in meters must be entered to ensure that the ASP300S makes the appropriate corrections when calculating the proper pressure or vacuum Upgrading software New versions of the operating software are distributed on hard disk drives For any upgrade or update of the software the hard disk drive needs to be replaced This procedure needs to be performed by Leica Technic...

Page 49: ...u will be warned that data will be deleted If you agree on deletion ac knowledge the dialog box by clicking Yes Successful completion of data transfer will be indicated by an according dialog box Data will be saved in Leica Data Loading data from an USB memory stick What kind of data can be loaded from an USB memory stick is described in the section Saving data to an USB memory stick To load data ...

Page 50: ...S menu option You must be logged on at supervisor access level to proceed From the start screen press MENU in the MENU FUNCTIONS window there press the MORE button The MORE MENU FUNCTIONS window appears There press the REAGENTS button The SET UP REAGENTS AND WARNING THRESHOLDS window opens To add a reagent Press INSERT to display the keyboard Enter the new reagent name Press OK to confirm You will...

Page 51: ...ocation to the wrong reagent group can lead to reagent cross contamination The respective threshold value applies for all reagent stations con taining the same reagent If a reagent is already used in a program it can neither be renamed nor can it be allocated to another reagent group The corresponding symbols will be disabled i e they will not be surrounded by a blue border If a reagent is renamed...

Page 52: ...arning thresholds for the selected reagent are automatically reset to 0 Adding new reagents to stations Go to SMART SCREEN to remote fill the reagent container from an external bulk container or fill the reagent container manually After filling a bottle manually the bottle must be defined as full To do so follow these steps Press the MENU icon to call up the MENU FUNCTIONS window Press the REAGENT...

Page 53: ...43 shows all programs defined in the ASP300S 5 Operation Important New infiltration programs are created by copying an existing pro gram Therefore the list must contain at least one program at all times Retort or paraffin cleaning programs are preset They cannot be renamed added or deleted Program duration cannot be specified It is determined by the total duration of all program steps plus the est...

Page 54: ...ROGRAM NAME at the top of the table to display the keyboard Enter the new program name Editing program steps Pressing EDIT in Fig 43 calls up the PROGRAM STEPS screen In the headline you will find the PROGRAM NAME The colors on the left border of the table indicate the reagent groups to which the reagents belong The program steps are displayed in the order in which they are carried out For each pr...

Page 55: ...ica ASP300 S Editing program steps To edit a step highlight the corresponding line and press the respective headline In the entry windows that pop up enter select the program step settings Fig 45 5 Operation ...

Page 56: ... program without having to recreate those steps Deleting program steps To delete a step from a program Highlight the step you wish to delete Press CLEAR Setting a delay step A delay step is a step that is extended to ensure that a program will finish at a specified time Highlight the program step you wish to define as delay step Touch the DELAY button The delay symbol is moved to the selected step...

Page 57: ...RAMS button Select the program you want and press OK to confirm Setting the end time Press the END TIME button In the input window specify the end time using 24 hour notation Select NOW if the program is to be terminated without generating a waiting time setting Confirm the end time with OK Fig 46 Fig 47 5 Operation If an end time is assigned to a Favorite the delay step is prolonged by the progra...

Page 58: ...esponding symbol is displayed which helps the operator recognize a certain program Press the ICON key in the table header Select the symbol you want and press OK to confirm Fig 49 Fig 48 The first symbol in the list is empty you can select this option if you do not want any symbol The Favorite will only be removed from the FAVORITES list the program itself will not be deleted Deleting a Favorite H...

Page 59: ...the instrument Fig 50 Allocation of the reagents Stations 1 10 can only be assigned specimen processing reagents other than paraffin Station 11 can only be assigned a cleaning solvent Station 12 can only be assigned a cleaning alcohol Stations 13 and 13 ext can only be assigned cleaning water detergents Wax baths may only be used for paraffin 5 Operation Only those reagents that are compat ible wi...

Page 60: ...d Press the COLOR button in the table header the SELECT COLOR window appears Select the color you want and confirm with OK To exit the display without changing any colors select CANCEL The newly configured color will now be used on all stations to represent the reagent In this window Fig 51 the colors used in the graphical program display for each reagent group are displayed ...

Page 61: ...p 4 3 to the connection 7 Fig 52 7 5 Operation Important Press the connection of the hose firmly into the holder until you can clearly hear it click Hook the other end of the hose into the bulk container that you are emptying or filling Reagents must have been checked for sufficient storage life Never use expired reagents While performing a reagent fill drain ensure that the remote fill drain hose...

Page 62: ...at no reagent has leaked Select station Press button Filling draining reagents From the FAVORITES screen press MENU In the MENU FUNCTIONS window tap the SMART SCREEN button The SMART SCREEN Fig 53 pops up The smart screen is the initial screen for manual operation of the instrument Fig 53 Fig 54 Status bar displayed in supervisor mode Status bar The following values are displayed Pressure and temp...

Page 63: ...ly in order to continue 17 36 From FAVORITES call up the SMART SCREEN screen see previous chapter Fig 56 5 Operation Use caution when handling melted paraffin molten paraffin is hot and may cause burns When connecting the hose be sure to press it onto the O rings of the drain opening as far as it will go The paraffin drain hose must be securely hooked into the external receptacle and remain there ...

Page 64: ...ets Donotinsertthebaffleplateduringtheheating phase The pellets need a total melting time of approx 10 hours 1 2 Fig 57 Important The level in a wax bath must NEVER fall below the mark for minimum level item 2 Fig 58 Replace the baffle plate when the paraffin is melted Software control After refilling with paraffin pellets the ASP300S does not allow you to run any pro grams until the paraffin is c...

Page 65: ...ir After a paraffin reservoir is filled with paraf fin it must be marked as full in the REAGENT STATUS table A safety prompt ap pears that you have to confirm with YES In the time window enter the desired de lay time and confirm with OK Fig 59 5 Operation Change the delay time only if you are absolutely sure that the entered time is correct If the instrument attempts to run a paraffin step with pa...

Page 66: ...ion has been activated Starting a Favorite To begin working with a Favorite press the corresponding symbol on the start screen The program is started immediately After starting the end time or other program op tions can be changed in exactly the same way as for any other running program FAVORITES ALL PROGRAMS Fig 61 Fig 62 To call up a program touch the button that is labeled with the program name...

Page 67: ...gram edit window appears Fig 64 Fig 64 Resetting the end time Touch the FINISH TIME button and reset the end time in the input window Fig 65 Enter the day on which you want the program to end Enter the time in 24 hour format at which you want the program to end Press OK to confirm 5 Operation Programs can be started up to 6 days in advance So that the program is ended at the defined date time the ...

Page 68: ...iodically pumped from the bottle to the re tort and back to achieve a more evenly mixed blend The first pump step begins after 16 min afterwards regularly after 12 min PRE TEST activated After the program is started the retort is filled and drained with the first reagent in the infil tration program to test whether all lines and valves are unobstructed STIRRER activated The magnetic stirrer is on ...

Page 69: ...display here will be updated accordingly To modify a running program or open the retort to reload specimens see Chap 5 3 3 Opening the retort the program has to be stopped press the PAUSE button All buttons that have changeable functions are acti vated and can be edited To change or delete program steps press EDIT and make the changes in the input window CONTINUE restarts the program The end time ...

Page 70: ...his prevents the reagents from spilling out of the retort when it is opened The signal tone and the 10 second waiting time occur each time the retort is unlocked For this reason we recommend only locking the retort before starting a program or before cleaning in order to prevent unnecessary waiting times The retort lock is equipped with a safety system for unlocking the retort Electronic emergency...

Page 71: ... has been unlocked using the mechanical emergency unlock Notify a responsible Leica service technician Once the stop has been overcome the retort can be opened Fig 74 4 and the samples can be removed and secured Notice A large amount of force is needed to do this Caution Risk of injury Fig 74 3 Approx 45 4 Aside from this the system is also equipped with a mechanical emergency unlock a predeter mi...

Page 72: ...s Reagent warning messages If the RMS is active warning messages are out put if reagents are used for too long The output of the warning messages can take place after up to 3 criteria for normal reagents and after up to 6 criteria for paraffins Warning messages are displayed at the end of a cleaning program Then you can switch directly to the REAGENT STATUS display Warning messages also appear wit...

Page 73: ...e maintained by Leica Service after approx 1000 operating hours AGE OF THE CARBON FILTER Displays the operating hours of the active carbon filter The configured warning value determines the triggering of a warning message The carbon filter must be replaced after approx 3 months Fig 76 5 Operation FILL DRAIN The table shows the measured fill ing and drain times of all stations in seconds as average...

Page 74: ...5 6 The online help The Leica ASP300S has an online help that can be started from every main window It contains a reference to the Instruction for Use The online help can be accessed from any screen by pressing the HELP button 5 Operation Fig 78 ...

Page 75: ...ongoing infiltration program the Leica ASP300S calculates the power failure duration into the total dura tion of the step taking place in other words the respective step is not lengthened due to the power failure If the power failure continues for longer than the remaining duration of the step during which it occurred the Leica ASP300S does not shorten the following steps If on account of a power ...

Page 76: ...idually test operations such as fill drain pressurizing and evacuation operations If you cannot identify the problem by carrying out the above listed steps call Leica Technical Service Power failures If there is evidence of power to the instrument Verify that the power plug is plugged into the socket and that the socket is switched ON Check whether the bridge cable is plugged in properly Verify th...

Page 77: ...ge in the air or reagent lines Blockages in the reagent lines are typically caused by paraffin or tis sue debris If there is reagent in the retort that cannot be drained try to remove the blockage as follows Heat the retort to the maximum possible temperature consistent with the reagent currently in the retort if any Leave the retort at this temperature for at least 15 minutes While the retort is ...

Page 78: ...order in which they are carried out fig 73 The clean programs skip any steps not required to complete the clean If e g an infiltration program has been aborted during the alcohol step the subsequent clean program will skip the cleaning solvent step and will start with the cleaning alcohol step Fig 79 Three retort cleaning programs and a paraffin cleaning program are defined in the ASP300S Clean pr...

Page 79: ...ene 7 Cleaning and Maintenance The finish times of clean programs cannot be edited Program dura tion is calculated automatically based on the number of cycles set The station 13 ext can be assigned exclusively to reagents from the cleaning water group It may only be used for cleaning programs Station 13 ext The designation 13 ext is used for an additional station that can be used in place of the s...

Page 80: ...ps are taken during the paraffin cleaning The paraffin is pumped into the retort There it is heated to the highest possible tem perature The retort is put under vacuum causing the solvent vapors to be drawn out of the paraffin The duration of the paraffin cleaning process is programmable To do so follow these steps InthePROGRAMSmenu selectthePARAFFIN CLEANING line and press the EDIT button In the ...

Page 81: ...l reagent containers are correctly inserted and connected All internal tubing is cleaned with compressed air to force any remaining reagents back into the reagent containers Phase 2 Before phase 2 starts the operator is prompted to remove all reagent containers from the instrument All internal tubing is again cleaned with compressed air Phase 3 When phase 3 starts the operator is prompted to place...

Page 82: ...eservoirs are very hot and may cause burns Do not use solvents on painted surfaces and or the touchscreen Wipe the paraffin reservoirs and lids clean The lid can be removed for cleaning purposes If they are dirty remove the wax strainers from the paraffin reservoirs Clean dry and reinsert them Ensure that the air vent hole at the top rear right hand corner is unob structed Instrument exterior Clea...

Page 83: ... a smart clean prior to chang ing the filter Fig 84 Draining the condensate container Remove empty and reinsert the blue conden sate container Fig 83 Fig 83 Filter label 7 Cleaning and Maintenance 14 13 Dispose of waste solvents with care according to local regulations and the waste management policy of the com pany or institution Do not operate without having an ac tive carbon filter installed Wh...

Page 84: ...damage to the retort lid seal and to the PTFE coat ing on the retort lid Do not damage the edges of the seal with the scraper Cleaning the retort The retort may be wiped clean using a cloth moistened with solvent xylene or alcohol or mild detergent In particular make sure that the air vent holes at the top front of the retort are not dirty Cleaning the retort sieve Use alcohol or xylene to clean t...

Page 85: ... bottle seal lubrication To ensure easy removal of the reagent and condensate bottles lubricate the O ring seals on the plug in nozzles with the O ring lubricant supplied 7 Cleaning and Maintenance This procedure is particularly important with bottles containing chloroform O rings that have not been lubricated swell when ex posed to chloroform The reagent containers can then become very difficult ...

Page 86: ...rear inner wall of the re agent module Failure to correctly plug reagent containers into the manifold will cause an interruption to the processing run and may result in spilling of reagents While the reagent bottles are outside of the reagent cabinet wipe the stainless steel inner walls of the reagent cabinet with a damp cloth moistened with a mild detergent Additional cleaning and maintenance tas...

Page 87: ... every cleaning program NEVER use the cleaning tool WITHOUT the accompanying microfiber cloth as other wise the sensors will be scratched The cleaning tool has two different spoons The larger spoon Fig 85 46 is for use with the ASP6025 the smaller Fig 85 47 is for use with the ASP200S ASP300S To prevent damage to the sensors NEVER use the larger spoon for cleaning the sensors in the ASP200S ASP300...

Page 88: ...ng program wipe the retort and cover dry Clean the outer surfaces of the instrument with a soft cloth and a very small amount of xylene Check the retort filter screen for tissue or paraffin residue Remove paraffin residue from the interior surface of the paraffin reservoir cover Check the retort lid seal and clean it if necessary Check the paraffin reservoir lid seal and clean it if necessary Chec...

Page 89: ...the condensate container Clean the inlet openings Check the status of the active carbon filter Replace the active carbon filter Check the level sensors and clean if necessary Check the electrical ports on the rear side of the instrument Clean the reagent containers from the inside if necessary Make sure that the shutter caps of the reagent containers and the O rings fit correctly and are tight Che...

Page 90: ... The warranty terms of your Leica sales organization or the organiza tion from which you have purchased the contractual product shall apply exclusively Service information If you are in need of technical customer support or spare parts please contact your Leica representative or the Leica dealer where you purchased the instrument Please provide the following information Model name and serial numbe...

Page 91: ...om within the product menu This template has to be used for gathering all required data When returning a product a copy of the filled and signed confirmation has to be enclosed or passed on to the service technician The responsibility for products that are sent back without this confirma tion or with an incomplete confirmation lies with the sender Returned goods that are considered to be a potenti...

Page 92: ...rder to prevent the transmission of contaminants and to avoid damaging the tissue samples Leica recommends Using sponges to affix small tissue samples for processing or packing them in filter paper Packing small biopsies into Leica CellSafe biopsy capsules biopsy sponges or biopsy bags Insufficiently prepared specimens can lead to the intrusion of artifacts into important instrument components and...

Page 93: ......

Page 94: ...www LeicaBiosystems com Leica Biosystems Nussloch GmbH Heidelberger Straße 17 19 D 69226 Nussloch Phone 49 6224 143 0 Fax 49 6224 143 268 Web www LeicaBiosystems com ...

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