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Leica ASP300 S, Operating Manual

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Leica ASP300 S Operating Manual Download Page 8

8

Operating Manual V 1.0 – 07/2006

2.

Safety

Transport and Installation

The instrument may only be transported in an upright position.
Follow the unpacking instructions carefully to avoid damage to the instrument!
A cleaning must be performed prior to each transport, during which the instrument may
be shaken, tilted or lifted. Severe internal damage may otherwise occur.

The instrument MUST be connected to an earthed mains power outlet socket. The instru-
ment must not be connected to an extension cord without protective earth conductor.
Ensure uniform current supply according the to set voltage!
The set voltage CANNOT be changed by the user.
Severe damage may occur if the instrument is connected to a power supply voltage other
than that to which it was originally set.

The instrument must be set up in a well ventilated area, free from any ignition sources.
The chemicals to be used in the Leica ASP300 S are both flammable and noxious.

Do not operate the instrument in rooms with explosion hazard.

If there is a significant difference in temperature between the warehousing and the in-
stallation site of the instrument and if at the same time there is a high air humidity level,
condensation water may form. In this case, a waiting period of at least two hours must be
observed before the instrument is switched on. Failure to adhere to this waiting period
may result in damage to the instrument. Failure to adhere to this waiting period may re-
sult in damage to the instrument.

Warnings - Markings on the instrument itself

Markings on the instrument showing the warning triangle indicate that the correct
operating instructions (as defined in this manual) must be followed when operating or
replacing the item marked. Failure to adhere to these instructions may result in an acci-
dent, personal injury, damage to the instrument or accessory equipment.

Some instrument surfaces become hot during operation.
They are marked with this warning label. Touching these surfaces may cause
burns.

Summary of Contents for ASP300 S

Page 1: ...art Processor Automated vacuum tissue processor Operating Manual Leica ASP300S V1 0 English 07 2006 Always keep this manual together with the instrument Read this instruction manual carefully before working with the instrument ...

Page 2: ......

Page 3: ...nt manual are not to be considered as warranted characteristics of our products These are determined only by the contract provi sions agreed between ourselves and our cus tomers Leica reserves the right to change technical specifications as well as manufacturing process es without prior notice Only in this way is it pos sible to continuously improve the technology and manufacturing techniques used...

Page 4: ...Electrical connection 20 4 3 Installing the accessories 22 4 4 Setting up the data connections 23 4 5 Alarm functions 24 4 6 Switching the instrument on 25 4 7 Touchscreen functions 27 4 8 Checklist for first operation 28 5 Operation 29 5 1 Setting up the instrument parameters 29 5 1 1 System setup 29 5 1 2 Access levels 32 5 1 3 INSTALLATION menu 34 5 1 4 Editing the reagent list 37 5 1 5 Viewing...

Page 5: ...1 General 60 6 2 Power failure 60 6 3 Troubleshooting 61 6 4 Typical fill or drain problems 62 7 Cleaning and Maintenance 63 7 1 Clean programs 63 7 1 1 Retort clean programs 63 7 1 2 Wax clean program 65 7 1 3 Smart Clean 66 7 2 General cleaning steps 67 7 2 1 Daily cleaning and maintenance 69 7 2 2 Periodic cleaning and maintenance 71 7 3 Preventative maintenance schedule 72 8 Warranty and Servi...

Page 6: ...operated according to the present instruction manual The Leica ASP300 S is a modular automated tissue processor designed for the following labo ratory applications fixation dehydration paraffin wax infiltration of histological tissue specimens The Leica ASP300S must be operated exclusively with the reagents listed in chapter 3 5 Compatible reagents 5 ENTER Qualification of personnel The Leica ASP3...

Page 7: ...vice personnel may repair the instrument and access the instrument s internal components 2 2 Warnings The safety devices installed in this instrument by the manufacturer only constitute the basis for accident prevention Primarily responsible for accident free operation is above all the institution which owns the instrument and in addition the designated personnel who operates services or repairs t...

Page 8: ...e Leica ASP300 S are both flammable and noxious Do not operate the instrument in rooms with explosion hazard If there is a significant difference in temperature between the warehousing and the in stallation site of the instrument and if at the same time there is a high air humidity level condensation water may form In this case a waiting period of at least two hours must be observed before the ins...

Page 9: ...rized air is used to clear the hose after each fill drain After refilling replacing the reagent bottles ensure that the lids are tight The bottles must be properly pushed home into the connection manifolds at the rear inner wall of the reagent module Failure to correctly plug the reagent bottles into the manifold will cause an interruption to the processing run and may result in spilling of reagen...

Page 10: ... very hot as well Dispose of waste solvents with care according to local regulations and the waste man agement policy of the company or institution Do not clean reagent bottles in an automatic dishwasher they are NOT dishwasher proof 2 Safety Warnings Cleaning and maintenance Prior to each maintenance and or cleaning switch the instrument off and disconnect mains power Do not clean the instrument ...

Page 11: ...ent protection Overcurrent conditions are protected against by both the mains fuse and the separate heating power fuses Overheating protection An error is indicated and all heating is stopped by the microprocessor control if the instrument detects any of the following conditions abnormally high temperature 75 C contradictory results of the temperature sensors failure of one or more heating power c...

Page 12: ...12 Operating Manual V 1 0 07 2006 3 1 Overview instrument components 3 Instrument components and specifications Fig 2 2 3 4 5 13 6 7 14 15 16 17 18 19 20 21 22 12 11 8 10 9 1 23 ...

Page 13: ...ction 18 Remote drain connection 19 Retort 20 Wax baths 21 Specimen basket 22 Lid for specimen basket 23 Splash protection 3 Instrument components and specifications Instrument components and accessories The processor module contains three paraffin wax baths a processing retort and also a touch screen with integrated key symbols as well as the electronic components The cassettes are held in three ...

Page 14: ...ll reagent bottles from bulk containers via the retort and a hose plugged into the processor module while the user is fully protected against coming into contact with reagents Remote wax bath fill drain feature Optical fluid level sensor Active paraffin wax solvent removal feature that increases the life of the wax by extracting and condensing contaminating solvents Magnetic stirrer to gently circ...

Page 15: ...ivated carbon filters assy 0476 34150 1 Magnetic stirrer complete 0476 34202 1 Paraffin wax scraper 0476 35923 14 Reagent bottles plastic 13 in instrument 0476 34274 1 Condensate collector plastic in instrument 0476 34278 1 Drip tray in instrument 0476 34145 1 Splash protection 0476 34770 1 Maintenance kit 2 replacement lids 9 o rings 0476 35921 1 Maintenance kit for pump in instrument 0476 35922 ...

Page 16: ...weight unpacked approx 160 kg Weight packed 220 kg Operating temperature range 15 C to 35 C Relative humidity 10 to 80 non condensing IEC 1010 classifications Protective class 1 Pollution degree 2 Overvoltage installation category II 800 V impulse 120 V systems 1500 V impulse 240 V systems Altitude 2500 m maximum Local remote alarm relays 30 V DC maximum 2 A 2 connections Both single voltage free ...

Page 17: ...djustable Impregnation vacuum 70 kPa g Impregnation pressure 35 kPa g Fill vacuum 70 kPa g Drain pressure 35 kPa g General Reagent bottles 10 Cleaning solution bottles 3 Maximum bottle volume 5 0 l Printer Optional accessory Pretest check ON OFF Fluid level sensor ON OFF Recirculation pump in out ON OFF a Time before 1st cycle 16 min b Time between cycles 20 min System setup Password status Superv...

Page 18: ...tection system Capacities 15 programs that consist of up to 10 reagent and 3 paraffin wax steps Time per program step 0 to 99 hours 59 minutes Delay time max 7 days Up to 300 cassettes can be processed simultaneously Four user programmable retort clean programs Wax clean program 10 reagent bottles 3 wax baths 3 cleaning solution bottles 1 condensate bottle Reagent temperature adjustable from 35 C ...

Page 19: ... com ponents of the instrument Do not use acetone benzene or trichlorethane in the instru ment Fixatives containing mercuric salts acetic or picric acid will corrode metallic components in the instrument and shorten instrument life If you choose to work with such fixatives it is essential to perform a clean cycle which contains multiple water rinses each time after use to minimize damage In additi...

Page 20: ...ort crate it must be touched only at the points marked with dots for transport to its final site of installation Once the instrument has been installed there the brakes on the instrument rollers 24 must be locked 4 1 Site requirements The instrument needs approx 650 x 700 mm floor space Practically vibration free floor Room temperature constantly between 10 C and 35 C Relative humidity maximum 80 ...

Page 21: ...socket It is recommended that the ASP300 S be plugged into a wall socket that has Ground Fault Circuit Interruption GFCI protection as an additional electrical safeguard Make sure to use the appropriate mains cable for the local voltage supply wall outlet Connect the mains cable 37 to the mains power supply socket 41 The jumper cable 38 which is supplied as part of standard delivery connects the m...

Page 22: ...ed next to the instrument during remote filling and draining the hose may be shortened to improve handling and the fill drain rate If the hose is shortened a vee notch can be cut into the hose end to en sure a clear passage for fluid flow Fig 6 28 Place the instrument in the definitive installa tion position Activated carbon filter Unpack the activated carbon filter and insert it into the instrume...

Page 23: ...estandardsisessential aspeciallyshield ed printer cable will be required Other connections For additional data transfer and storage the instrument console is equipped with a stan dard 1 44 MB floppy disc drive 11 and an RS 232 serial interface 12 Fig 8 Magnetic stirrer Unpack the magnetic stirrer 30 Fig 7 and insert into the retort Important Insert the magnetic stirrer so that the convex side 32 o...

Page 24: ...phono jack 34 supplied Ø 6 3 mm Local alarm socket 9 Remote alarm socket 10 Remote alarm This alarm is also external to the Leica ASP300S If installed it might typically be connected to a remote dialer that sends an automated phone message to the person responsible for after hours problems The remote alarm is only generated when the in strument cannot continue with a Processing pro gram Note that ...

Page 25: ...trument MUST be connected to an earthed mains power outlet socket It is recommended that the ASP300 S be plugged into a wall socket that has Ground Fault Circuit Interruption GFCI protection as an additional electrical safeguard Fig 11 Fig 10 Connect the instrument to the mains power outlet socket If applicable switch the mains power outlet socket ON Switch the ON OFF switch item 42 Fig 2 located ...

Page 26: ...neers only 2 Access INSTALLATION menu 3 Abort the current program 4 Abort the current program and delete the cur rent allocation of reagents to reagent bottles and retort 5 Delete all programs and reset instrument sta tus all lists will be empty 6 Store actual instrument status on floppy disk 7 Display run log 8 Restart instrument To exit this menu the instrument has to be re started press RESTART...

Page 27: ...they are enabled or disabled A button is disabled if the function that it performs is not applicable at the moment Disabled Enabled Disabled icons have a thinner border than active ones as shown here If a disabled button is pressed a help text will be displayed explaining why this particular button is disabled Button symbols Press the button to activate the corresponding function on the Leica ASP3...

Page 28: ...printer optional Set all parameters as required especially wax bath set temperature Check whether date and time are correct Add the desired reagents and edit the warning thresholds when REPLACE REAGENTS will be displayed Allocate a reagent to each station Fill the reagent stations bottles either via re mote drain fill function SMART SCREEN or manually REAGENT STATUS Create the programs you need Bo...

Page 29: ... up into five selection fields Program Options Display Buzzer Date Time Security Instrument Fig 18 5 1 1 System setup In the window MORE MENU FUNCTIONS press SYSTEM SETUP Fig 16 From the start screen press MENU to go to the MENU FUNCTIONS screen then press MORE to go to the MORE MENU FUNCTIONS screen Fig 17 ...

Page 30: ...ial otherwise the order of wax baths will be managed by the RMS PROMPT FOR NUM BLOCKS Enabled or Disabled Enabled When starting a program the actual number of blocks must be entered This is recommended with the RMS being enabled Disabled Number of blocks can be entered optionally not mandatory to enter no of blocks DATE TIME Be sure to verify that the date and time entered do actually correspond t...

Page 31: ...DISPLAY OFF indicates the remaining time in min until the screen saver after the last user action is activated LCD CONTRAST can be set from 0 to 14 The BUZZER VOLUME can be set between 1 and 10 5 Operation Wax temperature and run options screen Fig 23 Access the RUN OPTIONS function to display the op tions selected for the cur rent program The options selected will be applied to ALL programs A pri...

Page 32: ...ttons are surrounded by a black border Supervisor access level Supervisors may perform all Operator functions and additionally create programs and perform the instrument set up functions At the Supervisor access level a status line is added to the SMART SCREEN functions containing information on the retort and wax baths see Fig 48 page 49 To access Supervisor level press SUPERVISOR enter the requi...

Page 33: ...bled All functions can be accessed at any time until the screen saver turns off the screen Entry keyboard The keyboard is displayed any time text needs to be entered The keyboard headline 1 tells you what kind of text to enter 30 characters can be entered into each entry field though sometimes not all characters entered can be displayed Fig 26 Important keys Shift To shift to upper case symbols Al...

Page 34: ...me Selecting a language Press INSTRUMENT NAME to display the key board Enter a name 20 characters maximum for the instrument The instrument name is also displayed on the FAVORITES screen Press LANGUAGE to open the SELECT LANGUAGE menu Select the desired language there and press OK Fig 27 A message box will prompt you to restart the instrument in order to display the user interface in the selected ...

Page 35: ...he instructions in the upgrade pack with care as they may contain additional version specific information as well as new or modified procedures parameters and data that must be loaded or modified to ensure that the new software version functions correctly 1 Make a backup copy of the instrument s current data You will need an empty formatted diskette for this purpose 2 Insert Disk 1 or similar of t...

Page 36: ... list of the printers that can be connected The Leica ASP300S works with any printers that are com patible with commercial PC s With a Centronics standard cable connect the printer to the printer port See chapter 4 4 Fig 8 In theSELECTTHEPRINTERTYPEwindow highlighttheprint er that is connected to the instrument and press OK If the printer driver you connected is not listed try a similar printer of...

Page 37: ...en You must be logged on at supervisor access level to proceed From the start screen press MENU to go to the MENU FUNCTIONS screen then press MORE The MORE MENU FUNCTIONS screen will appear Press REAGENTS The SET UP REAGENTS AND WARNING THRESHOLDS screen opens To add a reagent Press INSERT to display the keyboard Enter the new reagent name Press OK to confirm You will then automatically be prompte...

Page 38: ...shold altogether Press OK to confirm The respective threshold value applies for all reagent stations containing the same reagent Changing reagent names or reagent groups If a reagent is already used in a program it can neither be renamed nor can it be allocated to another reagent group The corresponding symbols will be disabled i e they will not be surrounded by a blue border If a reagent is renam...

Page 39: ...SMART FUNCTIONS to remote fill the reagent bottle from an external bulk container or fill the reagent bottle manually After filling a bottle manually the bottle must be defined as full For that purpose Press MENU to access the MENU FUNCTIONS screen Press REAGENT STATUS In the reagent list highlight the station reagent that has been filled manually Press SET AS FULL to mark the station as full Upon...

Page 40: ... of a program the duration of one or more individual program steps has to be modified Retort and wax cleaning programs are predefined They cannot be renamed added or deleted 5 1 5 Viewing the program list You can create Up to 15 processing programs 3 retort cleaning programs 1 wax cleaning program At supervisor access level the processing program names can be edited new processing programs can be ...

Page 41: ...at the top of the table to display the keyboard Enter the new program name Editing program steps Press EDIT refer Fig 38 to access the PROGRAM STEPS screen In the headline you will find the PROGRAM NAME The colors on the left border of the table indicate the reagent groups to which the reagents belong The program steps are displayed in the order in which they are carried out For each program up to...

Page 42: ...Manual V 1 0 07 2006 5 Operation Editing program steps To edit a step highlight the corresponding line and press the respective headline In the entry windows that pop up enter select the program step settings Fig 40 ...

Page 43: ...teps Highlight the step you wish to copy Press COPY If required modify properties of the step Remember that a program step cannot be copied if the program already contains the maximum number of 13 steps Use the MOVE UP MOVE DOWN buttons to move program steps up or down within an existing program without having to recreate those steps Deleting program steps To delete a step from a program Highlight...

Page 44: ...be altered Press PROGRAM NAME Select the desired program and press OK to confirm Setting a finish time Press FINISH TIME Enter the finish time 24 hour clock Select ASAP if the program finish time is not to be delayed Press OK to confirm Fig 41 5 Operation If a preprogrammed finish time is assigned to a favorite a de lay will automatically be inserted into the program at the nomi nated Delay Step t...

Page 45: ...a favorite makes it easy to recognize at any time Press ICON in the table header Select the desired symbol and press OK to confirm The first symbol in the list is empty select this option if you do not want to assign a symbol to a specific program Deleting a Favorite Highlight the Favorite you wish to delete Press DELETE The Favorite is deleted without any further queries The Favorite is only dele...

Page 46: ...ailable for selection Highlight the desired reagent and press OK to confirm The list below Fig 45 shows all stations allocated reagents currently defined in the instrument Fig 45 Allocating reagents Only processing reagents except paraffin can be allocated to stations 1 10 Only a cleaning reagent can be allocated to station 11 Only a cleaning alcohol can be allocated to station 12 Only cleaning wa...

Page 47: ...header the entry window SELECT COLOR pops up Select the desired color and press OK to confirm If you want to close the entry field without changing the color press CANCEL The new color will be used in all stations to symbolize the reagent group it has been allocated to In this screen Fig 46 you find the colors used for each reagent group in the graphic program display 5 Operation ...

Page 48: ...in hose to the remote drain connection 7 on the front of the instrument refer Fig 6 Chapter 4 3 page 22 Important Ensure that the hose is pushed all of the way onto the connection until a clear click is heard Put the loose end of the remote fill drain hose into the desired container for the drain fill operation as desired Fig 47 While performing a reagent fill drain ensure that the remote fill dra...

Page 49: ... reagent has been spilled Select station Press key symbol Fill drain reagents In FAVORITES press MENU In MENU FUNC TIONS press SMART SCREEN The SMART SCREEN Fig 48 pops up The smart screen is the initial screen for manual operation of the instrument The following steps should only be carried out by trained laboratory personnel experienced in handling reagents Fig 48 Fig 49 Status bar displayed in ...

Page 50: ...he paraffin drain hose is securely placed in the remote container and is kept in place until the completion of the operation as the hose is automatically purged with air upon completion of the drain The hose should therefore not be re moved from the remote container until this cleaning step is complete Fig 50 Select the wax bath to be drained and the re mote container Remote Fig 51 Then press STAT...

Page 51: ...sh protector during the heating phase The pellets will take a total of approx 10 hours to melt Important During the melting process the par affin level may NOT drop below the MIN level pos 2 Fig 53 Once the paraffin is completely molten rein sert the splash protector Software control Paraffin pellets have to be completely molten before the ASP300S allows any programs to be run The instrument softw...

Page 52: ... UNTIL WAX MELTED Only change the melting time if you are absolutely certain that the new time entered is correct If the instrument attempts a paraffin step with the paraffin only partly molten severe instrument malfunctions may occur Fig 55 5 Operation If filling with molten wax do not fill above the MAX level see Fig 53 on the wall of the wax baths After a wax bath has been filled it must be mar...

Page 53: ...vorite press the corresponding sym bol on the Favorites screen the program will be started immediately After the program has been started its finish time and or other program parameters can still be changed as with any other program FAVORITES ALL PROGRAMS Fig 56 Fig 57 Press the symbol corresponding to the pro gram you wish to start A graphic representation of the stations retort will be displayed...

Page 54: ...e program editing screen will open Fig 59 Fig 59 Redefine the program finish time Press FINISH TIME In the entry window set a new finish time Fig 60 Enter the day of the week when you want the program to be finished Enter the time in 24 hour clock when you want the program to be finished Press OK to confirm Programs can be started up to 6 days in advance For the program to finish at a specific dat...

Page 55: ...uid The first pump step is carried out after 16 minutes all subsequent pump steps in 12 minute intervals PRE TEST enabled After starting the program the first reagent is pumpedintotheretortandbackintothebottle to verify whether all tubes and valves are unobstructed STIRRER enabled Magnetic stirrer is ON PRINTER if enabled At the end of the program run a full run log will be printed out Important T...

Page 56: ...accordingly Before a program in progress can be modified the PAUSE symbol must be pressed After pressing PAUSE all buttons surrounded by a blue bor der can be accessed edited To modify or delete program steps press EDIT and in the entry window carry out any changes you wish to make Press CONTINUE to restart the program The finish time of a program in progress can only be changed as long as the del...

Page 57: ... will give you warning messages when reagents have been used for too long Warning messages for normal reagents are giv en based on up to 3 criteria warning messages for paraffin wax on up to 6 criteria The warning messages are displayed at the end of a cleaning cycle From there you can go di rectly to the REAGENT STATUS screen In addition warning messages on a yellow back ground are displayed in t...

Page 58: ...a Technical Service carry out a preventive maintenance of the pump CARBON FILTER AGE Indication of hours of service of the activated car bon filter A warning message is displayed accord ing to the warning threshold set The carbon filter must be exchanged after approx 3 months of use Fig 65 5 Operation FILL DRAIN This screen displays the average fill drain times of all stations in seconds short ter...

Page 59: ...o back to the screen from where you accessed Help The CUSTOM HELP button 3 will only be displayed if user specific help files have been installed 2 3 Detailed information on how to create and install customer specific help files can be found in the What is Custom Help help text In addition to the Help Index there are context specific help texts These texts are displayed only when Help is accessed ...

Page 60: ...ica ASP300S counts the time during as contributing to the step duration i e the step is not prolonged by the power failure The Leica ASP300S does not however reduce the duration of subse quent steps should the power fail duration exceed the duration of the step in which the power failure occurred If the power failure has been long enough that the wax temperature may have dropped below a level suit...

Page 61: ...EEN functions to individually test opera tions such as fill drain pressurizing and evacuation opera tions Power failure If there is evidence of power to the instrument Verify that the mains lead is plugged in to the wall socket and that the wall socket is switched ON Check whether the bridge cable is plugged in properly Verify that the power switches on the rear of the instrument adjacent to the p...

Page 62: ... blockage in the air or reagent lines Blockages in the reagent lines are typically caused by wax or tissue de bris If there is reagent in the retort that cannot be drained try to remove the blockage as follows Heat the retort to the maximum possible temperature consistent with the reagent currently in the retort if any Leave the retort at this temperature for at least 15 minutes Whilst the retort ...

Page 63: ...three and a maximum of four steps 1 Cleaning Solvent 1 step from station 11 2 Cleaning Alcohol 1 step from station 12 3 Cleaning Water 1 step from station 13 Optional step between steps 2 and 3 Cleaning Water Detergent 1 step from station 13 ext The steps of retort clean programs are listed in the order in which they are carried out fig 68 The clean programs skip any steps not required to complete...

Page 64: ... set The finish times of clean programs cannot be edited Program duration is calculated automatically based on the number of cycles set Station 13 ext The term 13 ext stands for an additional station which can be used instead of station 13 Station 13 always contains cleaning water Some applications require the use of a cleaning water detergent mixture between the cleaning alcohol and the cleaning ...

Page 65: ...a retort clean before starting the paraffin clean A paraffin clean consists of the following steps The paraffin is transferred into the retort In the retort the paraffin is heated to the maximum temperature The retort is evacuated to draw off the xylene vapor The duration of the wax clean program is pro grammable For that purpose On the View Edit Programs screen highlight the wax clean program and...

Page 66: ...tainers are correctly inserted and connected All internal tubing is cleaned with pressurized air to force any remaining reagents back into the reagent bottles Phase 2 Before phase 2 starts the operator is prompted to remove all reagent bottles from the instrument All internal tubing is again cleaned with pressurized air Phase 3 Before phase 3 starts the operator is prompted to insert an empty bott...

Page 67: ...he wax strainers from the wax baths Clean dry and reinsert them Ensure that the air vent hole at the top rear right hand corner is unobstructed Instrument exterior Clean the instrument exterior as necessary Wipe with a damp cloth moist ened with mild detergent and dry Do not use solvents on painted surfaces and or the touch screen 7 Cleaning and Maintenance Regularly check the drip tray 16 for spi...

Page 68: ... replacing the filter press open the menu SMART MONITOR to reset the filter age to 0 See also chapter 5 5 Prior to changing the filter we recommend a smart clean Fig 73 Draining the condensate bottle Remove and empty the blue condensate bot tle and reinsert Fig 72 Dispose of waste solvents with care according to local regulations and the waste management policy of the company or institution Fig 72...

Page 69: ...retort lid seal and to the PTFE coating on the retort lid Do not damage the edges of the seal with the scraper Cleaning the retort The retort may be wiped clean using a cloth moistened with solvent xy lene or alcohol or mild detergent Ensure that the air holes at the top front of the retort are open Cleaning the retort strainer Use alcohol or xylene to clean the strainer located at the bottom of t...

Page 70: ...nd condensate bottle seal lubrication To ensure easy removal of the reagent and condensate bottles lubricate the O ring seals on the plug in nozzles with the O ring lubricant supplied This procedure is particularly important with bottles containing chloroform O rings that have not been lubricated swell when exposed to chloroform The reagent bottles can then become very difficult to remove Checking...

Page 71: ...t the rear of the reagent cabinet The reagent bottles must be properly engaged in the home po sition in the connection manifolds at the rear inner wall of the reagent module Failure to correctly plug reagent bottles into the manifold will cause an interruption to the processing run and may result in spilling of reagents While the reagent bottles are outside of the reagent cabinet wipe the stainles...

Page 72: ...ith soft cloth and minimal amount of xylene Check retort filter screen for tissue or wax residue Remove residual paraffin from inner surface of wax bath lid Inspect and if necessary clean retort lid seal Inspect and if necessary clean wax bath lid seal Inspect and if necessary adjust paraffin levels Inspect reagent container levels 7 3 Preventative maintenance schedule 7 Cleaning and Maintenance b...

Page 73: ...es if necessary Examine activated carbon filter Replace carbon filter Check fluid level sensors and clean if necessary Inspect electrical connectors at the rear panel of the instrument Inspect and if necessary clean inside reagent bottles Check that the bottle caps and seals of the reagent bottles are in place and tight Check that the reagent bottles are fully inserted in the connection manifolds ...

Page 74: ...t concluded The terms of warranty of the Leica sales organization or other organization from which the contract product was purchased shall apply solely Service information If you require technical service or need replacement parts please contact your Leica sales representative or dealer who sold this product Please provide the following information Model name and serial number of the instrument L...

Page 75: ...75 Leica ASP300 S Notes ...

Page 76: ...76 Operating Manual V 1 0 07 2006 Notes ...

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