Jan 2012
T190 Iss.2
Page 12 of 38
4.4.
Gas connection
Risk of injury. Compressed gas.
Risk of damage to unit.
Observe relevant regulations for handling
CO
2
Risk of asphyxiation. Site must have adequate ventilation.
Risk of
CO
2
poisoning. Site must have adequate ventilation.
The incubator is designed for use with CO2 of 99.5% purity. Input pressure greater than 1.0 bar (
15
psi)
will damage the incubator so a pressure reducing valve (PRV) must be used.
The CO
2
inlet to the incubator is via a 4mm barbed push fitting.
PRV’s with the correct fittings and other gas accessories can be supplied by LEEC.
Section 10
For correct connection of CO
2,
follow these steps in order:
1) Connect the supplied tubing and HEPA filter to the gas supply.
2) Fully close the PRV.
3) Open the main valve on the gas bottle or central supply.
4) Adjust the PRV pressure of the gas supply to between 0.3 bar and 0.7 bar (
4.5 to 10 psi
).
5) Close the main valve on the gas bottle / central supply.
6) Connect the other end of the tubing to the incubator CO
2
inlet. Secure with the supplied clip.
Repeat these steps whenever you change the CO
2
bottle or supply.
Turn off the main valve and disconnect from the CO
2
supply if the incubator is to be unused for extended
periods.