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Chapter 4: Performance Features and Safety Precautions

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UV Sash Closure

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Summary of Contents for 3970202

Page 1: ...Purifier Filtered PCR Enclosures User s Manual Labconco s Mascot Labby the LABster Models 3970202 3970205 3970222 3970225 3970302 3970305 3970322 3970325 3970402 3970405 3970422 3970425...

Page 2: ......

Page 3: ...and insurance charges In the event of a warranty claim contact Labconco Corporation or the dealer who sold you the product If the cause is determined to be a manufacturing fault the dealer or Labconc...

Page 4: ...urface 9 Verifying HEPA Filter Integrity 12 Connecting the Electrical Supply Source to the Filtered PCR Enclosures 12 Setting the Downflow Velocity with Speed Control 13 Validating the Filtered PCR En...

Page 5: ...t 36 UV Light Replacement 37 Motorized Impeller Replacement 37 Speed Control Replacement 39 CHAPTER 7 ACCESSORIZING MODIFYING YOUR FILTERED PCR ENCLOSURE 40 CHAPTER 8 TROUBLESHOOTING SERVICER OPERATIN...

Page 6: ...tions as defined by Federal Standard 209E The enclosure is set to deliver nominal downward airflow of 45 65 feet per minute Each enclosure uses a single HEPA filter which is rated at least 99 99 effic...

Page 7: ...70 1 020 352 83 ISO Class 5 100 000 23 700 10 200 3 520 832 29 ISO Class 6 1 000 000 237 000 102 000 35 200 8 320 293 ISO Class 7 352 000 83 200 2 930 ISO Class 8 3 520 000 832 000 29 300 ISO Class 9...

Page 8: ...how to install use and maintain your Filtered PCR Enclosure Chapter 1 Introduction provides a brief overview of the Filtered PCR Enclosure explains the organization of the manual and defines the typog...

Page 9: ...a task are presented in a numbered format Comments located in the margins provide suggestions reminders and references Critical information is presented in boldface type in paragraphs that are preced...

Page 10: ...tionally the enclosure should be strategically placed in the lab to provide efficient workflow Carefully read this chapter to learn the requirements for your installation site The support vibration an...

Page 11: ...cause an air disturbance or be a nuisance Overhead or wall HVAC diffusers fans radiators or other lab equipment producing air currents Doorways or windows that may be opened Airflow Specifications Th...

Page 12: ...370 58 60 60 62 61 63 63 54 85 115 145 170 4 4 Filtered PCR Enclosure 30 40 50 60 240 330 420 500 60 62 62 64 66 69 68 70 85 115 145 170 Electrical Requirements Standard duplex electrical receptacles...

Page 13: ...th the speed control adjustment Validate enclosure airflow Seal the enclosure to the work surface Depending upon which model you are installing you may need common mechanical and electrical installati...

Page 14: ...nts and tested the enclosure We recommend that you do not remove the enclosure from its shipping container until it is ready to be placed into its final location Move the unit by placing a flat low do...

Page 15: ...ea for containing primary spills Filtered PCR Enclosure Work Surface Installation 1 Level the base cabinet or stand and the work surface See Figure 3 1 2 Position the work surface in its intended loca...

Page 16: ...11 Product Service 1 800 522 7658 Chapter 3 Getting Started Figure 3 1 Filtered PCR Enclosure Installation...

Page 17: ...esign ensures Class 5 conditions and a particulate free environment for PCR experiments HEPA Filter Appropriate Use Filter Size 2 3 4 Part No 3707900 3707901 3707902 HEPA filters are high efficiency p...

Page 18: ...w velocity See Figures 6 1 and 6 2 in Chapter 6 to locate the speed control The downflow velocity is increased by turning the speed control counterclockwise and clockwise to decrease downflow velocity...

Page 19: ...the Work Surface When the Filtered PCR Enclosure has been set in place and wired it may be sealed at the work surface to prevent materials from collecting under the walls A bead of silicone sealant is...

Page 20: ...of 30 to 65 feet per minute The HEPA filter is jacketed by negative pressure preventing contaminated air inside the enclosure and ensuring Class 5 air conditions inside the enclosure After each Polyme...

Page 21: ...16 Chapter 4 Performance Features and Safety Precautions Figure 4 1 6 10 9 Located Behind 4 2 1 12 5 3 6 Located Behind Control Panel 15...

Page 22: ...7 Chapter 4 Performance Features and Safety Precautions Figure 4 2 Filtered PCR Enclosure Airflow Diagram 13 19 6 Airflow Switch with Adjustment Screw 15 UV Light Not Shown 7 11 18 4 16 Not Shown 1 17...

Page 23: ...18 Chapter 4 Performance Features and Safety Precautions Figure 4 3 UV Sash Closure 16...

Page 24: ...re included with the User s Manual See Figure 4 1 and 4 2 6 Optional Guardian Airflow Monitor continuously monitors airflow An audio visual alarm alerts the user to low airflow conditions The Guardian...

Page 25: ...er airfoil The UV light timer is variable with eight settings in minutes of 5 10 15 30 60 120 240 and infinity This provides extreme flexibility for PCR protocol It is easily changed by selecting the...

Page 26: ...apparatus inside the lower air foil of the enclosure 6 Do not work in this enclosure without the blower running 7 Perchloric acid use in this enclosure is prohibited 8 Radioactive materials are prohi...

Page 27: ...ure is connected to an electrical service in accordance with local and national electrical codes Failure to do so may create a fire or electrical hazard Do not remove or service any electrical compone...

Page 28: ...use it Read this chapter to learn about 1 Routine Daily Work Procedures 2 Suitable Applications 3 Appropriate HEPA Filter Applications Suitability and Guidelines Routine Daily Work Procedures Planning...

Page 29: ...contaminated materials in the work area Final Purging Upon completion of work the enclosure should be allowed to operate for two to three minutes undisturbed to purge airborne contaminants from the wo...

Page 30: ...ring sample protection from environmental contamination HEPA filters are only effective for entrapment of particulate matter The surface of the HEPA filter is fragile and should not be touched Care mu...

Page 31: ...ter 6 Review this chapter on maintenance for the following 1 Routine Maintenance 2 Determination of when to replace the HEPA filters 3 How to install a new HEPA filter 4 HEPA filter leak test 5 Speed...

Page 32: ...d apparatus Airflow alarms are recommended for constant monitoring All weekly activities Check downflow velocity Increase speed control or change HEPA filter when downflow velocity of the enclosure dr...

Page 33: ...clamp bolts loosened the HEPA filter clamping frame should be clear of filter Carefully pull the filter straight out of the enclosure and discard properly 5 Cover the surface of the new HEPA filter g...

Page 34: ...iltered PCR Enclosure Figure 6 1 HEPA Filter Changing Diagram Filter Leak Test Diagram Prefilter Filter Clamp Bolt HEPA Filter Sealing Surface HEPA Filter Gasket Side Down as Shown Gasket Front Panel...

Page 35: ...dge Dr Owings Mills MD 21117 2 One aerosol generator of the Laskin nozzle s type An aerosol of mineral oil or suitable liquid shall be created by flowing air through it The compressed air supplied to...

Page 36: ...s 8 If necessary adjust the speed control of the enclosure to maintain the following downflow airflows at 55 fpm 2 230cfm 3 340 cfm 4 460 cfm 9 Position the aerosol generator discharge in the intake o...

Page 37: ...ibrate and Operate the Airflow Monitor Optional Guardian Airflow Monitor Refer to Figure 6 2 for operation and calibration Labconco Airflow Monitor Airflow Switch Operation The Guardian Airflow Monito...

Page 38: ...al inputs and having the following possible functions External Alarm allows an external signal to sound the alarm from the building airflow system of a failed condition Alarm Disable allows an externa...

Page 39: ...mized 7 The table below lists typical alarm conditions based on normal operating conditions Typical alarm conditions are set at downflow velocities of 10 to 30 feet per minute below the normal operati...

Page 40: ...igure 6 2 Guardian Airflow Monitor with Airflow Switch Light Reflector Speed Control Airflow Monitor LED Circuit Board Airflow Switch with Adjustment Screw Control Panel Green Safe LED Silence Alarm B...

Page 41: ...ified at least annually The certifier should perform the minimum following tests Downflow Velocity Test HEPA Filter Leak Test In addition the following tests should also be performed at the user s dis...

Page 42: ...bly The HEPA filter rests below the motorized impeller assembly 1 Unplug the enclosure from the electrical outlet 2 Remove HEPA filter per the HEPA filter removal procedure outlined earlier in this ch...

Page 43: ...placement 2 Model Mounting Screws for Motorized Impeller HEPA Filter Capacitor Mounted here on Electrical Subassembly located behind the Control Panel Motorized Impeller Wire Access Hole Impeller Moun...

Page 44: ...2 Remove the two screws holding the speed control using a Phillips screwdriver Refer to Appendix A for Replacement Parts Diagram 3 Disconnect all wires leading to the speed control Connect wires on n...

Page 45: ...lters and storage cabinets or stands 1 Work Surfaces An optional dished work surface is available to attach to the Filtered PCR Enclosure Dished work surfaces are contoured to fit the dimensions of th...

Page 46: ...0 Airflow Sensor w Connector 2 ea 1885405 6 32 x 31 Phillips machine screw to mount Airflow Monitor Circuit Board 2 ea 1891808 6 32 x 50 Phillips thread cutting screw to mount Airflow Sensor 1 ea 3935...

Page 47: ...Hinge Dual Doors Right Hinge Left Hinge 48 9900000 36 9900100 30 9900200 24 9900300 9900600 18 9900400 9900700 9907000 9907100 12 9900500 9900800 48 w Self Closing Doors 36 w Self Closing Doors 30 w S...

Page 48: ...niques for the enclosure See Certifying the Enclosure Chapter 3 and Safety Precautions Chapter 4 sections in the manual Ref Appendix D External factors are disrupting the enclosure airflow patterns Se...

Page 49: ...ripped Reset or replace circuit breaker Lights do not work Lamp not installed properly Inspect lamp installation Lamp wiring disconnected Inspect lamp wiring Defective lamp Replace lamp Light switch i...

Page 50: ...ocity The HEPA filter may have become loaded If downflow velocity is correct calibrate the airflow monitor outlined in this manual in Chapter 6 Monitor alarms air way to airflow monitor sensor is bloc...

Page 51: ...46 Chapter 8 Troubleshooting Service Operating Record Log Customer Name Model Number Date Installed Serial Number Unit Location Date Comments...

Page 52: ...U UR RE E C CO OM MP PO ON NE EN NT TS S A AN ND D R RE EP PL LA AC CE EM ME EN NT T P PA AR RT TS S The components that are available for your Filtered PCR Enclosure are listed The parts shown are th...

Page 53: ...OD 9C 2 7868402 Bushing Spacer 31 x 63 10 1 9 Ft 6913700 Wiper Sash Seal 11A 2 1936800 Bushing Heyco closed 1 50 Dia 11B 2 1934601 Bushing Heyco w Flex Shutter 1 50 Dia 12A 2 3940500 Hinge outer 12B 2...

Page 54: ...ler Motorized 2 or 3 Enclosure 230V 24C 2 3934600 Impeller Motorized 4 115V 24D 2 3934601 Impeller Motorized 4 230V 25A 1 1306900 Capacitor 2 115V 10 MFD 25B 2 1306900 Capacitor 3 115V 10 MFD 25C 2 13...

Page 55: ...x 3 32 w Gasket 32C 1 3707902 HEPA Filter 4 42 x 18 x 3 32 w Gasket 35A 1 1333800 IEC Power Inlet 115V 35B 1 1305800 Power Cord 115V Right Angle 35C 1 1305900 Power Cord 230V Right Angle 35D 1 133920...

Page 56: ...arts 24 26 Fluorescent 25 27 UV 23 15 7 14 40 13 35 36 Not Shown 28 31 32 20 29 30 11 16 9 10 1 6 3 2 4 5 12 Not Shown 19 17 18 8 UV Light Timer Located Behind Control Panel 22 Figure A 1 Replacement...

Page 57: ...52 A AP PP PE EN ND DI IX X B B D DI IM ME EN NS SI IO ON NS S See the following dimensions for the Purifier Filtered PCR Enclosures...

Page 58: ...53 Appendix B Dimensions and Exhaust Options Figure B 1 Purifier Filtered PCR Enclosure...

Page 59: ...perature range 41 to 104 F 5 to 40 C Maximum relative humidity 80 for temperatures up to 88 F 31 C decreasing linearly to 50 relative humidity at 104 F 40 C Main supply voltage fluctuations not to exc...

Page 60: ...55 Appendix C Filtered Enclosure Specifications C 1...

Page 61: ...56 Appendix C Filtered Enclosure Specifications C 2...

Page 62: ...57 Appendix C Filtered Enclosure Specifications C 3...

Page 63: ...58 Appendix C Filtered Enclosure Specifications C 4...

Page 64: ...ronmental Sciences 1986 IES Recommended Practice IES RP CC 001 96 Laminar Flow Clean Air Devices Mt Prospect IL Institute for Environmental Sciences 1986 IES Document IES CC 011 85 T A Glossary of Ter...

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