7
Contents
SECTION 1 - INTRODUCTION ............................................................................. 9
1.1
INSTRUMENT DESCRIPTION........................................................................................ 9
1.2
GENOVA NANO WITH MICRO VOLUME ACCESSORY SPECIFICATION .................. 9
1.3
UNPACKING.................................................................................................................. 10
SECTION 2 – ACCESSORY LAYOUT AND INSTALLATION ........................... 11
2.1
ACCESSORY LAYOUT ................................................................................................. 11
2.2
INITIALISATION ............................................................................................................ 11
Note: The instrument will return to the last main menu used. ...................... 11
SECTION 3 – MICRO-VOLUME SETTINGS ...................................................... 12
3.1
ACCESSING THE MICRO VOLUME SETTINGS ......................................................... 12
3.2
PATH LENGTH SELECTION ........................................................................................ 13
3.2.1
Known Path Length Measurements .............................................................................. 13
3.2.2
Unknown Path Length Measurements .......................................................................... 13
3.2.3
Auto Path Length Availability ......................................................................................... 13
SECTION 4 – PERFORMING MICRO VOLUME MEASUREMENTS................. 14
4.1
PIPETTING SAMPLES ONTO THE MICRO VOLUME READ HEAD........................... 14
4.2
SAMPLE RECOVERY OR REMOVAL .......................................................................... 15
SECTION 5 – TOP 10 TIPS FOR SAMPLE MEASUREMENT ........................... 16
SECTION 6 – STEP BY STEP GUIDE TO MAKING A DNA MEASUREMENT . 17
6.1
dsDNA Mode ................................................................................................................. 17
6.2
A260/280 Mode ............................................................................................................. 17
6.3
Multi-wavelength Mode .................................................................................................. 18
SECTION 7 – CALIBRATION OF THE MICRO VOLUME ACCESSORY .......... 19
7.1
CALIBRATION SOLUTIONS (035 092) ........................................................................ 19
7.2
MICRO VOLUME CALIBRATION PROCEDURE ......................................................... 19
7.2.1
Micro Volume Settings ................................................................................................... 19
7.2.2
Calibration Standard - Data Entry .................................................................................. 19
7.2.3
Calibration and Verification ............................................................................................ 20
SECTION 8 – CLEANING AND DECONTAMINATION ...................................... 25
8.1
CLEANING..................................................................................................................... 25
8.2
DECONTAMINATION .................................................................................................... 25
8.3
READ HEAD RECONDITIONING ................................................................................. 25
SECTION 9 – ACCESSORIES ........................................................................... 26
9.1
ACCESSORIES ............................................................................................................. 26
SECTION 10 – MAINTENANCE AND SERVICE ............................................... 26
10.1
ROUTINE MAINTENANCE ........................................................................................... 26
10.2
SERVICE ....................................................................................................................... 26
SECTION 11 – TROUBLESHOOTING ............................................................... 27
11.1
CALIBRATION ERROR CODES ................................................................................... 27
