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SECTION 4 – PERFORMING MICRO VOLUME MEASUREMENTS
For detailed descriptions of the measurement modes that are available on the Genova Nano
spectrophotometer please refer to the supplied Genova Plus operating manual.
4.1
PIPETTING SAMPLES ONTO THE MICRO VOLUME READ HEAD
The Genova Nano spectrophotometer is designed to measure sample volumes ranging from
0.5
µ
l to 5.0
µ
l.
Jenway recommends that users should, if possible, use at least 2
µ
l of sample for their
measurements.
Pipetting a sample onto the read head
1. With the read head mechanism open,
pipette the liquid to be analysed onto the
centre of the lower read head. Ensure
there are no bubbles in the sample.
Read head in rest position
2. Close the lid of the spectrophotometer.
This will lower the read head assembly
down onto the path length drive motor. The
upper read head will now be in the rest
position, 2mm above the lower read head.
Read head in measurement position
3. When a measurement is initiated, the path
length drive motor lowers the upper read
head to the specified measurement
distance, the photometric measurement is
taken and the upper read head is then
returned to the rest position.
