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Operating Manual 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

737 555 REV D/08-16

 
 

Genova Nano Spectrophotometer 

 

 

 

 

Summary of Contents for Genova Nano

Page 1: ...Operating Manual 737 555 REV D 08 16 Genova Nano Spectrophotometer...

Page 2: ...r safe handling of chemicals should be employed Do not use hazardous or flammable substances in the instrument 5 If it is suspected that safety protection has been impaired in any way the unit must be...

Page 3: ...tent 6 Le symbole d alerte signale l utilisateur les informations importantes concernant l utilisation de l appareil Lire et suivre les instructions fournies avec la plus grande attention 7 Cet appare...

Page 4: ...UV Lichtquelle zur ck Nicht direkt in die Lichtquelle schauen 8 ACHTUNG Wenn das Ger t nicht in der vorgegebenen Weise eingesetzt wird k nnen die Schutzfunktionen des Ger tes beeintr chtigt werden 9 A...

Page 5: ...rnita dal dispositivo stesso potrebbe risultare compromessa 9 Non sostituire i cavi di alimentazione di rete scollegabili con cavi inadeguati Lea esta informaci n atentamente antes de instalar o utili...

Page 6: ...fallo debe comunicarse inmediatamente a la autoridad de servicio de mantenimiento y reparaci n pertinente 6 El s mbolo de advertencia avisa al usuario de informaci n importante relacionada con el uso...

Page 7: ...LES ONTO THE MICRO VOLUME READ HEAD 14 4 2 SAMPLE RECOVERY OR REMOVAL 15 SECTION 5 TOP 10 TIPS FOR SAMPLE MEASUREMENT 16 SECTION 6 STEP BY STEP GUIDE TO MAKING A DNA MEASUREMENT 17 6 1 dsDNA Mode 17 6...

Page 8: ...1 Calibration Procedure Error Codes 27 11 1 2 Verification Check Error Codes 27 11 2 TROUBLESHOOTING GUIDE 28 11 3 TECHNICAL SUPPORT 28 SECTION 12 GLOSSARY OF ICONS 29 SECTION 13 CHEMICAL COMPATABILI...

Page 9: ...mmed methods for protein analysis such as the Bradford Lowry Biuret BCA and Direct UV methods This life science spectrophotometer uses icon driven software and has an improved navigation system for ea...

Page 10: ...power supply 24V 65W 021 060 Calibration standards with certificate 035 092 Genova Nano instruction manual 737 555 Genova Plus instruction manual 736 505 The Genova Nano is delivered with the micro vo...

Page 11: ...trument and connect to the mains socket Turn the power on at the mains and switch the instrument on using the power switch on the rear of the instrument The instrument will initially check for firmwar...

Page 12: ...e micro volume settings allow the user to select the required path length 0 2mm or 0 5mm for a measurement and to calibrate the accessory using a standard solution with known absorbance values at 260...

Page 13: ...auto path length setting has been selected press the button adjacent to the tick icon to confirm The auto path length measurement setting will firstly measure a sample using the 0 5mm path length set...

Page 14: ...ting a sample onto the read head 1 With the read head mechanism open pipette the liquid to be analysed onto the centre of the lower read head Ensure there are no bubbles in the sample Read head in res...

Page 15: ...ophotometer The read head mechanism will open to allow access to the upper and lower read heads 2 The sample can be recovered by carefully drawing the liquid that is retained on the lower read head in...

Page 16: ...ample which would normally read an absorbance of 1 0 in a standard 10mm cuvette will only give a value of 0 05 at 0 5mm or 0 02 at 0 2mm 7 To minimise any factors which may interfere with a reading su...

Page 17: ...Menu icon and check that the wavelength is set to 260nm the units are g ml and F1 50 3 Lift the lid of the Genova Nano and pipette 2 l of water onto the read head Close the lid Perform the Blank Readi...

Page 18: ...he Settings Menu icon Check that the settings are as follows Wavelength 1 260 Wavelength 2 280 Wavelength 3 230 Activate the reference Wavelength 4 320nm The units are g ml 3 Touch the button next to...

Page 19: ...e standards as advised in the MSDS 7 1 CALIBRATION SOLUTIONS 035 092 The supplied calibration solution set consists of two vials 1 Matrix Blank White 2 10x ref Calibration Standard Blue A calibration...

Page 20: ...rd Path Length Value icon 4 Select the digit to be changed using the keys at the bottom of the screen Use the keys adjacent to the Arrow icons to increase or decrease the number Press the key adjacent...

Page 21: ...will return the instrument to the micro volume settings menu screen The unit takes three dark and light readings at 260 and 330nm at both 0 5mm and 0 2mm path lengths 3 The Blank Solution Measurement...

Page 22: ...0 006 0 050 A 5 0 5341 0 4899 A 2 0 2279 0 1959 The readings at this stage are validated by examining the Absorbance at 330nm used for background correction of the readings at 260nm The background cor...

Page 23: ...e brackets the Passes Test icon will be displayed Press the button adjacent to the Tick icon to continue or the button adjacent to the Cross icon to abort the calibration process 15 The new calibratio...

Page 24: ...ads Open the instrument lid and pipette a 2 0 l aliquot of the calibration solution onto the lower read head Close the instrument lid and press the key adjacent to the Tick icon to initiate the calibr...

Page 25: ...level measurements A final cleaning of all surfaces with deionised water is also recommended after the last measurement 8 2 DECONTAMINATION If decontamination is necessary a sanitising solution such a...

Page 26: ...scientific com Tel 44 0 1785 810475 On occasion it may be necessary for your equipment to be sent back to our Service Department for repair In this case please contact the Service Department for a ref...

Page 27: ...ion process will need to be repeated The table below shows the error codes 11 1 1 Calibration Procedure Error Codes Error Code Symbol Issue 1 Blank solution reading outside of acceptable range 3 Calib...

Page 28: ...eve a reading when measuring a sample Ensure the correct path length is being used Ensure the sample isn t so dense so that light is not transmitted through the sample Ensure the lamp is working 11 3...

Page 29: ...mm path length setting selected Settings Auto path length setting selected Settings Path length calibration Settings 260nm Calibration standard absorbance value Settings 330nm Calibration standard abs...

Page 30: ...uret Potassium iodide 0 5 Biuret Sodium hydroxide 0 08M Lowry Sodium carbonate 1 6 Lowry Copper II sulphate 0 032 Lowry Sodium potassium tartrate 0 016 Lowry Sodium dodecyl sulphate 0 08 Lowry Sodium...

Page 31: ...31 Bibby Scientific Ltd Beacon Road Stone Staffordshire ST15 0SA United Kingdom Tel 44 0 1785 812121 e mail info bibby scientific com www bibby scientific com...

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