jenway 7315, Operating Manual

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SECTION 9 – Kinetics
The kinetics measurement mode enables the absorbance or % transmittance of an active molecule to be
measured over a period of time; for example enzyme analysis of horseradish peroxidase. The absorbance
or % transmittance is measured at regular time intervals at a set wavelength over a period of time.
The results are plotted on a graph to show the change in absorbance or % transmittance over time.
Following sample measurement statistical analysis of all or part of the experiment can be performed.
9.1 MODE SPECIFIC PARAMETERS
The kinetics minimal operating menu enables calibration
to zero absorbance and kinetics measurements to
be performed without changing any measurement
parameters or settings. Pressing the key adjacent to the
JW
icon opens the expanded operating menu.
The kinetics expanded operating menu enables
measurement parameters to be changed. The utility
toolbar on the left hand side of the screen enables access
to printing, print setup options, results, methods and
autologging options. For more details on the different
functions of the utility toolbar refer to section 10.
The settings icon enables the wavelength, units, resolution, graph y-axis, absorbance or % transmittance
operating mode, standard, factor, measurement time, lag time and start on level to be set. Following
the completion of a set of kinetics measurements the
statistics
for all or part of the kinetics run can
be analysed. The kinetics moving line function enables the initial and final points of the curve to be
selected, so that analysis of the area between these two points can be performed.
Fig 9.1.1 - Expanded Operating Menu – Post Measurement
Minimal Operating Menu
Expanded Operating Menu
3.000
ABS
-5.000
0.00 10.00 5.0 s
09:02
3.000
ABS
-5.000
0.00 10.00 5.0 s
09:02
3.000
ABS
-5.000
0.00 10.00 5.0 s
09:02
Print/print settings
Settings
Kinetics moving line
Statistics
Calibrate
to zero
Start kinetics
Measurement
Results selection menu
Method selection menu
Autolog menu