iota Sciences isoHub, User Manual

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shielding plate) is not engaged in
the light path.
A part of the field of
view is not visible or is
dark. Or, the brightness
of the field of view is
not even. The phase contrast slider is not
correctly engaged in the light
path. Match the phase contrast slider
correctly with the light path
position (see section
7.1 ).
The objective is not correctly
engaged in the light path. Engage the objective in the light
path correctly (see section
7.3 ).
The dish holder or well plate
holder is blocking the light path. Move the dish holder or well plate
holder out of the way of the light
path (see section
4.2 ).
(Fluorescence observation) The
fluorescence mirror unit is not
properly engaged in the light
path. Engage the fluorescence mirror
unit in the light path correctly.
(Fluorescence observation) The
direction of the filter slider for
reflected light illumination which
is inserted to the fluorescence
illuminator is not correct. Set the direction of the filter slider
for reflected light illumination
correctly.
(Fluorescence observation) The
centring of the CoolLED light
source is not performed. Adjust the centring of the
CoolLED pe4000.
(Fluorescence observation) The
objective is not correctly
engaged in the light path. Engage the objective in the light
path correctly.
Dust or dirt is visible in
the field of view. Dust or dirt is left on the
eyepieces. Clean the eyepieces.
Dust or dirt is left on the optical
element. Clean the optical element.
Dust or dirt is left on the
specimen. Clean the specimen, or replace it
with a clean specimen.
One side of the field of
view blurs. The specimen is tilted on the
stage. Set the specimen correctly on the
stage. Ensure the 60mm dish
holder or well plate holder are
attached properly (see section
3.2.7 ).