Hettich AG, Seestrasse 204a, 8806 Baech, Switzerland
Operator_Manual_Rotolavit II_und_Rotolavit II-S_en_rev2.5
Seite 36
Decanting:
The number of revolutions per minute is adjustable
from 0 rpm to 2500 rpm. The excess is decanted at
the selectable rotation speed. During decanting, the
rotor turns in the opposite direction of the normal
rotation so that the solution will be decanted. The
default-value is 370 rpm.
Agitating, shaking:
The numbers of shakings are adjustable from 0x to
500x. The default value is 15x. The pellet is loosened
again for the subsequent wash cycle by a fast, brief
movement of the rotor and the tube holders.
9.4.6 DECANT process
The correct speed must be validated with the used tubes for the best possible results because the
different inner surfaces of the tubes, e.g. glass tubes or plastic tubes. You will also get different results
when the speed is optimized for 10mm tubes but the used tubes are 12mm tubes.
If the DECANT speed is too high then it is possible that the washed cells will also be decanted.
If the speed is too low, you will probably have too much liquid in the tubes and with the next FILL
process the tubes will be overfilled.
9.4.7 AGIT process
