CHAPTER 5
OPERATION OF THE JUNIOR-PAM
<Status>
The <Status> field provides access to the 7 central functions of the JUN-
IOR-PAM fluorometer. A checkbox is associated with each function
listed. Checkboxes represent on-off switches but they also report the
status of measuring and actinic light when the WinControl-3 software
runs the JUNIOR-PAM automatically. Settings of all 7 fluorimeter func-
tions are stored on a microcontroller in the JUNIOR-PAM fluorometer
and kept until power supply is disconnected.
The fluorometer functions are:
<Meas. Light> Low frequency measuring light.
<ML-F high> High frequency measuring light. To become active,
<Meas. Light> needs to be checked. Note that measuring light
changes automatically to high frequencies at actinic light intensities
higher than level 3 and during saturating light pulses.
<SAT-Pulse> The commend initiates a saturating pulse analysis to de-
termine F’, Fo’ and Fm’: hence, the <SAT-Pulse> function corre-
sponds to that of <SAT> in Box (6).
<Act. Light> Actinic light.
<Far Red> Far Red Light.
<Fo’-Mode> Follow up a saturating pulse with an interval with actinic
light switched off and far red illumination switched on, and take the
fluorescence yield measured at the end of this interval as Fo’ fluores-
cence.
<Clock> Trigger a process with the interval defined as <Clk. Width> in
the <Basic> field (this Box) or in <Settings> (see below). Also, the
event to be triggered is defined in <Settings>.
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